Non-GMO Water Lab Report

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

The vital components and techniques of gene cloning are as follows, the DNA sequence that contains the desired gene (EZH2) is amplified by Polymerase chain reaction. PCR was established by Kary Mullis in 1985, popularly known to amplify target sequences of DNA (EZH2) to a billion fold in several hours using thermophilic polymerases (Taq) ,primers and other cofactors (Sambrook and Russell, 2001). Three crucial steps are involved which are Denaturation (at 95°), Annealing of the forward and reverse primers (55-65°) and lastly primer extension (at 72°). After amplification the desired sequence is integrated into the circular vector (pbluescript) forming the recombinant molecule. For the compatibility of the insert and vector, both were digested with (EcoR1) so the same cohesive ends are generated in both, making it easier to ligate. EcoR1 is a restriction enzyme that belongs to the type II endonuclease class which cuts within dsDNA at its recognition site “GAATTC” (Clark 2010; Sambrook and Russell, 2001).

In the Chemistry of Natural Waters Lab we were to collect a sample of water, ranging from a fountain, stream, bottle, or tap water. After we collected the samples we all did many tests to see what the hardness was for each one. Water hardness is determined by the amount of Calcium and Magnesium in the water.(2) Water that has more Calcium or Magnesium is considered to be harder than water with less of those two elements. When you use soap and detergent, this is where you see water hardness coming into play in everyday life when you are washing things.

The purpose of this experiment is to observe the natural process of osmosis using drops of sheep’s blood in various saline solutions. Since the cell membrane is a selective membrane, the water goes in and out freely but a solute such as NaCl it cannot freely move in or out. Osmosis is a passive process that doesn’t require the input of energy to transport water molecules across a membrane. The channels that allow water to enter or leave a cell are known as aquaporin. Using aquaporin, water moves from a solution with a low solute concentration to a solution with a higher solute concentration. This process continues until equilibrium in solute concentration is established between a cell and its environment.

As we know that osmosis means diffusion or dispersion of water through a selectively permeable membranes from high concentration to a lower concentration and there are many different factors that can affect the rate of Osmosis such as temperature particle size and the size of concentration ingredient. As we know that higher temperature can cause osmosis to occur at much faster rate because a molecule are more likely to pass through the selectively permeable membrane quicker than they would at a lower temperature. In this experiment the sucrose molecule was too large to pass through the dialysis bag, while water molecule able to move freely in and out due to their small size. Lastly the congregation gradient can greatly affect the rate of osmosis

There were several steps used to acquire the colony necessary for the PCR. First a student forearm was swabbed using a cotton swab, the cells were then placed in an agar plate. DNA was then extracted from the cultured bacteria by using a technique to lyse the cells and solubilize the DNA, then enzymes were used to remove contaminating proteins. The DNA extraction consisted of a lysis buffer that contained high concentrations of salt for denaturing. Binding with the use of ethanol and a washing step to purify the DNA. The final step for the DNA extraction was elution where the pure DNA was release. Proceeding the extraction of DNA the results of the 16s gene amplification were examined through gel electrophoresis it was analyzed by estimating the size of the PCR bands with marker bands. After measuring the success of the extraction, a technique called TA cloning was started. Cloning of PCR products was done by using partially purified amplified products with

One binding theme amongst the five articles was whether or not America could break free from its discriminatory history and allow for a nation where individuals can freely accept and express their unique cultures and backgrounds.

The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for Genetically Modified Organisms, and this refers to any products that have been manipulated or altered at the gene level.

BoHV-1 genomic DNA was extracted using the TIANamp Genomic DNA Kit and Δ gD1 and Δ gD2 fragments were generated by PCR amplification using 2× GoTaq Master Mix under recommended setting. Each PCR product was purified using a Wizard SV Gel and PCR Clean-Up System by agarose gel electrophoresis. The purified PCR product, Δ gD1 or Δ gD2 was sequentially cloned into pET28a double digested by the corresponding restriction endonuclease, followed by transform into competent E. coli DH5α. The recombinant positive plasmid was confirmed by DNA sequencing was designated as pET28a-ΔgD1-ΔgD2.

Figure 1 Gel Electrophoresis for Replication Taster PTC. The gel is composed of an ethidium bromide stained 3% agarose gel demonstrating DNA fragments which were a depiction of PCR amplification. The agarose gel contains nine loading samples, including from left to right, the MW marker lane 1 precision mol mass standard, lane 2 TB undigested PTC (5µl of DNA, 5µl of master mix P, and 2.5µl of loading dye), lane 3 TB digested PTC (5µl of DNA, 5µl of master mix P, 2µl Fnu4HI, and 3µl of loading dye), lane 4 TB A(L)DH G (10µl DNA, 10µl master mix G, and 5µl loading dye), lane 5 TB A(L)DH A (10µl DNA, 10µl master mix A, and 5µl loading dye), lane 6 MG undigested PTC (5µl of DNA, 5µl of master mix P, and 2.5µl of loading dye), lane 7 MG digested PTC (5µl of DNA, 5µl of master mix P, 2µl Fnu4HI, and 3µl of loading dye), lane 8 MG A(L)DH G (10µl DNA, 10µl master mix G, and 5µl loading dye), lane 9 MG A(L)DH A (10µl DNA, 10µl master mix A, and 5µl loading dye).

Misty Mountain bottles natural water sourced from organic land for private entities. Our water is non-GMO verified. Our team provides co-packing, which allows private entities the ability to design their own label for branding and promotion. Our quality assurance team sticks to strict quality assurance standards to ensure a premium product with quality unmatched.

In this experiment, I concluded that amylase have the abilities to catalyze the breakdown of the starch into glucose. Also, some molecules, such as the Lugol’s regent (I2Kl) and glucose are able to permeate through intestine (Dialysis tubing) while others, such as starch is not able to permeate through the (Dialysis tubing).

Analysis of DNA from practicals 1 and 2 using the technique of agarose gel electrophoresis and analysis of transfomed E. coli from practical 2 (part B)

The chemical and reagents used for the extraction and quantitation of DNA were: Plant DNAzol (0.3ml/0.1g), 100% ethanol (100%: 0.225 ml/0.1 g, 75%: 0.3 ml/0.1 g), Chloroform (0.3 ml/0.1 g), Plant DNAzol-ethanol solution: Plant DNAzol, 100% ethanol (1:0.75 v/v), TE buffer (10 mM Tris, 1 mM EDTA pH 8.0), 1.2% agarose gel (Agarose, 1X TAE buffer), 6X loading buffer (glycerol, Tris/EDTA pH 8.0, ethidium bromide), .25X TAE buffer, Restriction enzymes and Restriction endonuclease buffers. All the chemicals used were quality grade. The restriction

This essay based on the principle of real time PCR which uses CYBR green dye that combines to any double stand DNA. This process included two maim steps. The first step was designing of HSV-1 primer and /or HSV-2 primer (we have chosen only HSV-1). BioEdit software has been used to edit the nuclide sequences where necessary. After that the primer has been ordered. The second step was in the laboratory which included applying the HSV-1 primers to real time PCR protocol. The final results of this protocols aim to demonstrate the quality of HSV-1 primer that we have designed by interpretation three criteria. These are specificity, efficiency and