Notes On Chromatography

2367 Words May 1st, 2016 10 Pages
Background:
Derived form the greek words for colour (Chroma) and writing (graphe). Chromatography is a method is method in which different kinds of the coloured chemical mixtures are separated. In the early 1900s, Mikhail Tswett, a Russian botanist, was interested in the individual compounds presented in plants. He understood that removing ground up plants extracts with the dissimilar solvents will provide assorted coloured solutions. Tswett conducted an experiment which involved pouring a plant extract through a glass tube filled with calcium carbonate. As the liquid passed through the powder, bands of colour was revealed; as individual compounds.



In chromatographic techniques, there is a stationary phase: usually a solid, thick liquid or bonded coating that stays in a secure position. mobile phase or eluent: usually a liquid or gas moves through it or across it.
The mobile phase carries the components of the mixture with it.
The separation of a sample, placed in the stationary phase, will gradually transport along with the mobile phase. If a sample compound has no connection with the stationary phase, it will come out as the same rate of mobile phase. If a sample compound has connection, it will stick with the stationary phase. This distribution ratio among the selected phases must differ for each sample compound in order for them to separate.

Types of Chromatography:
Paper Chromatography:
Paper chromatography is a analytical technique in which the mixture is…
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