Nt1310 Unit 2 Lab Report

Part A shows how limited the range of the Spectronic 20 spectrometer is. It did not measure transmittances below 400 nm or above 575 nm. This means the light source emitted those wavelengths of light so weakly they were not detected by the instrument.

Chlorophyll a had the highest absorbance rate resulting in readings peaking at 0.7. Xanthophyll had one of the lowest absorbance reading peaking between .2 and .22. Carotene had the second highest absorbance reading at 0.44 and the wavelength of 460. Chlorophyll b had a very close reading to that of carotene resulting in a peak between 0.38 and 0.41. Within the data of chlorophyll a and chlorophyll b, one will notice a general pattern of increase, decrease, and lastly a smaller increase. Carotene and xanthophyll have only one major peak within the absorbance

Aim: To see how chlorophyll pigments absorb electromagnetic radiation at different wavelengths, with the use of a spectrophotometer to vary the wavelength of incident light, and then plot an absorption spectrum.

The graph shows the relationship between wavelength and reflectance spectrum. In my experiment, I did on the grey calculator, the green book, and the red book. Each of these colors correspond to a specific wavelength of light. When we look at an object and see its color, it means that the object reflects more light in one part of the spectrum than the others. Looking at the graph, the red book has the peak percentage of reflectance lies in the area 620-700 nm of wavelength. That makes sense because the red wavelength interval is about 625-740 nm. Similar to red, in the visible spectrum ranges from 400 nm to 700 nm, the green book graph (520-530 nm) also corresponds to the green wavelength, which is about 500-565 nm. On the other hand, the graph

Colors affect the intensity of sunlight. The purpose of this lab was to see how different colors affect the intensity of the light in a given solution. During the lab, a calorimeter probe was used to study the relationship between concentration and light transmittance through solution samples and how light transmittance varies with the wavelength of light. In a calorimeter, specific light of a substance and intensity is passed through a solution. A detector then measures the amount of light that comes out of the other side. The light that is not transmitted is absorbed by the sample. If the concentration of the sample is greater then the intensity of light that passes through the sample is lower. The ratio of the intensity of light leaving

When a photon strikes an object, the photon may be absorbed, transmitted, or even reflected. When the photon is absorbed into the pigment molecule,

Light is a form of energy and composed of different wavelengths. When the light exposed to an object, the light could be reflected at the surface of the object (specular or diffuse), and it could be absorbed or scattered within the object, or it could refract or totally transmitted through the object44, 45. The wavelengths (colors) that are reflected, refracted or transmitted are perceived by receptor cells (i.e. rods and cones) in the eye and recognized by the brain as a specific color. The wavelengths of visible light range from approximately 400 to 700 nm45, 46. The wavelengths that are transmitted, refracted or reflected create the color that is perceived and till about the translucency level of the material. If all light is transmitted, the material will appear completely transparent. If all light is absorbed, the material will appear completely opaque, and the color black is perceived. However, if some of the wavelengths of visible light are absorbed and others reflected, refracted, or transmitted, the color that is perceived corresponds to the wavelengths that are reflected, refracted, or transmitted, and the level of translucency is depend on the amount of the light that transmitted through the material9, 44-46. Moreover, the reflection of the light depends on the surface texture of the restoration. Thus, a smooth surface increases the specular reflectance, in which the angulation of light reflection is equal to the angle of the light source. This reveals more of

The data from this experiment has the relationship , and by using the slope, the wavelength of the laser’s light can be calculated. The wavelength of the red laser pointer should be between 620nm and 670nm, and if the experimental value is within this range then the data can be assumed to be accurate.

Using a standard curve the concentrations of two unknown samples were calculated. The standard curves were created using the calculated concentrations of the standards and the mean absorbance obtained using Atomic Absorption Spectrometry. One of the unknown samples had a concentration outside of the standard curve and had to be diluted before it fell within the curve. .

An infrared spectrum is the plot of absorbance (or % transmission) against the wavenumber (cm-1) (Rehman et al., 2012). Wavenumber is defined as number of waves in a length of one centimeter and is a commonly used unit for representing spectral regions. Wavenumber is linear with energy and is expressed with the following equation.Significant parts of the spectrum are the regions where the molecule absorbs the infrared energy. These are shown as characteristic absorption peaks.

Fig. 4. (a) The phase parameter (Δ) and (b) shift parameters given by variable angle spectroscopic ellipsometer and the corresponding fitted model are shown. An example of the refractive index and extinction coefficient are illustrated in (c).

Table 1: Table of the BSA and dH2O volumes to be pipette and placed in the spectrophotometer to obtain absorbencies for the standard curve

The absorption coefficient decides how far into a material, the light of a particular wavelength can penetrate before it is absorbed.

To calculate landsat 8, land surface emissivity (LSE) first the following equation Eq. 3.4 is used to calculated proportion vegetation.

Thermo Scientific Genesys 10s UV-Vis spectrophotometer was employed to obtain the absorption spectra of test samples.