Ntspa 662 Torque

An unknown bacterium was handed out by the lab instructor. The methods that have been learned so far in identifying bacteria were applied to this unknown. Procedures were followed as stated in the lab manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that, specific biochemical tests were performed for gram positive, since unknown number five was determined to be gram positive rod. The other tests were performed in this order: Mannitol Salt (MSA) streak, Blood Agar streak, Catalase test, Nitrate Reduction test, and Phenyl

5 The test microscope slide was composed of a drop of B. subtilis solution placed on the left as the Gram-positive control, a drop of a solution with unknown in the middle, and a drop of E. coli solution on the right as a Gram-negative control. The slide was allowed to dry and the samples were heat fixed with a Bunsen burner. The samples were then stained with first crystal violet for one minute. After the rinsing the stain, the mordant, Gram’s iodine, was added for one minute and rinsed. A 95% ethyl alcohol/acetone solution was then applied and rinsed to de-stain the samples. Finally, Saffranin was added as the counterstain for one minute and then rinsed. Bright field microscopy, using 1000X magnification and oil immersion, was conducted to visualize first the controls, and if those results were accurate, the unknown. The three samples were observed for stain color to determine Gram classification, as well as shape and arrangement to further classify the

A mixed culture of two unknown bacteria was provided by the instructor. The methods used for

The purpose of this study project was to carefully isolate and identify two unknown bacteria from a mixed culture. The ability to properly evaluate biochemical test results is also necessary for the identification to be successful. The goal was to apply all of the methods and techniques that have been learned in the microbiology laboratory course for the proper identification of unknown bacteria. A certain amount of bacteria that were used throughout the course were possible bacteria that could be found in a mixed culture. The bacteria that were identified in the mixed culture were Staphylococcus Aureus and Kocuria Rhizophila.

In a laboratory setting, it often becomes necessary to identify an unknown organism. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. A Gram stain using crystal violet, iodine, and safranin and a simple stain using methylene blue characterized the organism’s cell wall. Cultural behavior was classified by inoculating the organism onto nutrient agar and incubating it at 37° C for 48 hours, and observing its behavior, as well as using SIM medium to test for motility. Optimal growth temperature was

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible

Today in medicine doctors are rapidly isolating and distinguishing the many pathogenic microbes encountered daily within the environment. Public health has been affected from the faster identification of microorganisms by delivering an accurate analysis to patients in order to receive treatment of the disease in a timely manner. Due to the growing understanding of these organisms more have been easier to indicate to improve water quality. Also more methods have been developed for better treatment options from fecal bacteria in public water systems. Scientist has developed such specific methods of identifying the unknown organism to tell if the contamination has come from either a human, bird, or mammal. (Achtman et al., 2008)

The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature, pH level, and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria can also be used in many other areas from better crop production through microbial pesticides to biological warfare. Their uses are endless as are their abilities to evolve and adapt to changing environments. That is why it is so important

There are many differents ways to identify a bacterial unknown and many different situations where identification would be beneficial. One way to identify bacterial unknowns is to perform biochemical tests. In this experiment multiple biochemical tests were done, by performing these tests on the bacterial unknown received the two different bacteria were then identified. The citrate test is done to test the ability of organisms to use citrate as a carbon source. This test uses Simmons citrate agar, the agar contains sodium citrate as the only carbon source and has bromothymol blue as the pH indicator. The organisms that use citrate as a carbon source use the enzyme to transport the citrate into the cell. The cells converts ammonium dihydrogen

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, catalase, lactose and sucrose fermentation, Kugler/iron agar, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, bile esculin,

The sole purpose of this project was to identify an unknown bacteria sample #7. Many tests were carried out to determine what this unknown was. Aside from a microbiology lab, understanding and identifying various organisms are important in disease processes, pharmaceutical arenas, and even in the industrial field. Proper lab techniques, including aseptic technique were used throughout the process of identification.

On June 25th, 2015 I chose the test tube labeled #19. This test tube contained an unknown bacterium, and the purpose was to determine the unknown bacterium by the end of the semester. Throughout the course, I ran a series of differential tests that would lead me to discovering the characteristics of my unknown. These tests that I will discuss in this paper are vital to understanding the biochemical mechanisms that different bacteria can perform, therefore helping me identify my bacterium based on molecular differences. During the course of this paper, I will refer to my unknown as unk#19. Also, I would note that aseptic technique was performed throughout the entire experiment and subcultures were regularly made.

Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

The purpose of this experiment is to obtain isolation of individual species of particles from the mixed culture. This is completed through the isolation technique of streak plate. The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. Because it is on a larger scale the particles are able to be visually observed as they are isolated using the streaking technique as the experiment is conducted. The benefits of the streaking technique is when a cultures has multiple species they are able to be more easily identified once they have been isolated. This experiment is much like the experiments completed on an agar plate but on one a much larger scale and where techniques