Nutrient Agar Lab Report

Use a glass beaker to pour an adequate amount of the liquid substance and use the pipette from the glass beaker.

In the lab we filled the first beaker up with water. Then we took a pipet (filled with the liquid) and dropped water droplets onto the

lab bench. Place a beaker from the drawer on the stir plate. Drag the bottle of NH3 to the 5 mL graduated cylinder (the smallest one) by the sink and fill the cylinder by dropping the bottle on the cylinder. Now drag the 5 mL graduated cylinder to the beaker on the stir plate and add the 5 mL of

Place the beaker on the hot plate, place the thermometer in the beaker and set the hot plate to 5oC.

1. Take a paper towel and cut out two circles that are the same size as the base of the petri dishes.

9) Trial E: Remove the syringe and empty the beaker. Add a Thermometer to the beaker. Add 200 mL of Room Temperature water to the beaker and heat with a Bunsen Burner until it reaches 100° C. Remove the Bunsen Burner. Repeat Steps 5 & 6.

Fill a test tube about 1/3 full with cold tap water for use in step 34.

1.) Measure out 20ml out of the water and place it into a glass beaker

4. Place 25 mL of the low concentration tobacco solution into the “low concentration” petri dish. 5. Place 25 mL of the medium concentration tobacco solution into the “medium concentration” petri dish. 6.

- Get 5 50mL beakers and fill the first one with 30 mL of water

XII. Take the 250 ml beaker to your lab bench. Set up a gravity filtration with a plastic funnel, folded wet filter paper, and an Erlenmeyer flask. Pour the content in the 250 ml beaker slowly through the filter paper. Wash the filter paper with deionized water. Dispose of the filtrate in the proper labeled waste container.

1) Pour 25 mL of the 1 M hydrochloric acid into the beaker and rinse the solid by swirling the acid around in the bottom of the beaker.

To start off the experiment, materials were gathered and placed on the table, those materials were 6 different essential oils. Peppermint, lavender, sweet orange, tea tree, lemon and eucalyptus were all used in the experiment. After collecting the materials, the containers were labeled with the name of each essential oil and the agar was then heated using the microwave until it turned into liquid. The agar was then placed in the six different petri dishes and add three drops of each of the essential oil to its labeled petri dish. After the agar had solidified and cooled down it was placed in a room temperature and pictures were taken. Growth would be observed every two days by taking pictures and if growth is present, then the names of bacteria

3. Use a sterile pipette to transfer 0.1 ml of each dilution on to a MacConkey agar plate.

1. Number four clean test tubes 1-4. 2. Place 1 ml (or 20 drops) of the following solutions into each tube: water, egg albumin, starch, and chicken broth. 3. Add 3 drops of Buiret reagent to each tube and gently shake