Oil-Eating Bacteria Lab Report

Lab, pilot, and field thinks about give direction on the utilization and usage of bioremediation on sandy shorelines, wetlands, and salt swamps. Different ventures show which factors are most critical for scattering of oil into the water section at all temperatures.

See Table 1 and Flow Chart 1 for results of Bacteria # 1 and Table 2 and Flow Chart 2 for results of Bacteria # 2.

coli were labeled as bacteria that should not be examined or touch without permission from the supervising teacher. Although the strain of E. coli used in this experiment was harmless, to avoid transmitting the bacteria to other organism, the experimenter handled the E. coli for caution (see Appendix H, I, J, K).

With NIT 3 probes hybridized bacteria could not be detected, thus either hybridization was unsuccessful or nitrate reducing activity by the NIT 3 gene was not present in the samples. Showing fewer artifacts, the Ntspa 662 probe showed no positive hybridization. Ntspa 662 is specific for the genus Nitrospira, which is known to conduct nitrite oxidation. For the BS 820 probe, which is specific for Candidatus Scalindua wagneri and C. S. sorokinii, no hybridization to bacteria was found, while for the more general probe Sca 1309 just one very small colony of Candidatus Scalindua specimen could be detected (figure 15, J - L) and a very high abundance of anammox bacteria of the genera Kuenia stuttgardiensis or Brocadia anammoxidans was verified using AMX 820 probe, especially in the seeding sludge samples. Thus the majority of anammox bacteria in the seeding sludge are not of the genus Candidatus Scalindua. Besides the positive FISH results, the shape of bacteria hybridized with AMX 820 was similar to what was initially seen with DAPI. Fluorescence reveals ring and sickle shapes (cf. figure

Bioremediation is the process in which microorganisms such as bacteria, yeasts, molds, and filamentous fungi are introduced into the spills. The introduction of these microorganisms assists in altering and metabolizing various chemical compounds present in oil. When the microorganisms metabolize these certain compounds it significantly reduces the time it takes for the oil biodegradation to occur.

On June 25th, 2015 I chose the test tube labeled #19. This test tube contained an unknown bacterium, and the purpose was to determine the unknown bacterium by the end of the semester. Throughout the course, I ran a series of differential tests that would lead me to discovering the characteristics of my unknown. These tests that I will discuss in this paper are vital to understanding the biochemical mechanisms that different bacteria can perform, therefore helping me identify my bacterium based on molecular differences. During the course of this paper, I will refer to my unknown as unk#19. Also, I would note that aseptic technique was performed throughout the entire experiment and subcultures were regularly made.

Finally, our results showed that the bacteria on the +pGLO plate with the amp and ara nutrients had the glowing bacteria. Even though the plate had only one colony of bacteria, it was the only one that glowed. This proves our hypothesis to be partially true because it shows how we were right in choosing the +pGLO plate with all the nutrients to have the glowing bacteria. In conclusion, our hypothesis was half right and half wrong, because the +pGLO with all the nutrients had the only glowing bacteria, but didn;t have the most colonies of bacteria like we first

Methods: In this experiment, results were found by discovering the kinds of bacteria found in the given pond water samples. The first step in this experiment was to prepare the pond samples by completing serial dilutions of the pond water to the 10-4. We then had to prepare five separate plates by streaking the 10-4 dilutes. These plates all had different treatments. The treatments were pond 16, pond 22, pond 22 with penicillin, pond 22 with ampicillin, and pond 22 with colistin sulfate. The plates that were treated with antibiotics required 0.1 mL of the designated

Oil constantly surrounds consumers throughout the world, whether it be in transportation, electricity, and heating. Since consumers use, transportation, electricity, and heating every day, the demand for oil has surged and thus, multiple companies race to provide this dark elixir promptly. As all thoughts concentrate on the speed in which oil becomes transported, reckless mistakes happen, which swiftly unleashes the toxic elixir into the ocean. The oil then resides in the water with death looming over its shoulder; countless birds, mammals, and fish become poisoned by the oil, which proves lethal. However, an antidote, colonies of microscopic bacteria that consume oil can be used to clean the oil pollution. Although oil satisfies humans with

Microorganisms are ubiquitous and play a large role in the environment. Microorganisms help some other organism in symbiotic relationships and they can also harm other organisms by causing them disease. Tests have been developed to help determine the characteristics of many microbes so that their special capabilities can be utilized for benefit. While these tests have also helped to develop substances and strategies to reduce the populations of microbes that have detrimental effects. The experiment that was performed used some of these techniques to determine the identity of some common microbes.

The Post Hoc multiple comparison in Table 5 shows that among the three bacterial cocktails, the most potent is PAECI-003. This is supported with the p value of the three different bacterial cocktails; this signifies that in the Null hypothesis of the study only the PAECI-003 bacterial cocktail should be rejected. Remarkably, the result of bacterial count supports that the number of bacteria is a factor to degrade the hydrocarbon present in a controlled amount. Generation time of the bacteria is also considered as a factor as Pseudomonas aeruginosa and Acinetobacter baumanii doubling time is 45 and 48 minutes respectively (Moffatt, Harper, Harrison, Hale, & Vinidradov, 2010). The result also showed that the PAECI-003, which is a bacterial cocktail

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a

Oil spreading method is based on that droplet of biosurfactant-containing solution can displace the oil and spread in the water. In drop-collapse method, the droplets contain biosurfactant spread or even collapse due to reduction of the force or interfacial tension between the liquid droplet and the hydrophobic surface. The stability of droplets is dependent on biosurfactant concentration and correlates with surface and interfacial tension [3,60]. In general, it was shown that hemolytic activity test has a relatively clear relationship with the qualitative oil spreading and drop-collapse tests. Hamzah et al. [20] reported that quantitative analysis including emulsification index and surface tension measurement are more reliable for detection of the presence of biosurfactant in the medium by bacterial isolates.

Although disease control is an aspect of intensive animal production, controlling disease in the aquatic environment is further complicated by the relationship between pathogens and their host and the frequent use of open production systems (Olafsen 2001) that discharge effluent directly into the environment. In many cases, bacteria of the genus Vibrio are opportunists, causing disease when the host organism’s immune system is suppressed or physiologically stressed, with the frequency of infection often being attributable to environmental conditions (Alderman and Hastings 1998).

Oil exploration clean-up techniques Techniques The Containment Boom Chemical Detergents The Sponge Bacteria Advantages Cheap and easy Disadvantages Only good when there are (35) ________________ ____________________ Good for treating (36) _________ Chemicals remain in the water + ___________________________ kill marine life. Oil remains permanently in the The sponge mats turn into sponge. (37) _______________________ ___________________________ (39) _______________________ Cheap Easy to administer Totally (38) _________________ ___________________________