Oleuropein Hypothesis

According to data, the natural antibiotics worked better for defending against Bacillus cereus. The Oregano Oil and the Garlic had tied for first and proved to have the largest zone with a length of 18 millimeters between the disc and the bacteria after 48 hours of growth. For a bacteria such as “Food Poisoning” the final result was shocking that the least amount of growth was for the man made antibiotics because they were specifically made to fight these types of Bacteria. The final result for Gentamicin had proved it to have a much smaller zone of inhibition with a zone of just 12 milimeters after the 48 hour time period. The growth of the penicillin was the lowest of them all with the very small zone of 0 millimeters after the 48 hours.

THE EFFECT OF INCREASING THE CONCENTRATION OF DETTOL ANTISEPTIC LIQUID ON THE GROWTH OF E.COLI

Modern-day advances have allowed scientists to develop methods to control pathogens. The more common methods include the use of antiseptics, antibiotics, and vaccines. By definition, antiseptics are chemicals used to kill pathogens. A few widespread antiseptics include soap, vinegar, and rubbing alcohol. Antiseptics are primarily used for the external destruction of pathogens, while antibiotics and vaccines target internal pathogens. Nevertheless, there are and can be difficulties concerning the use of antibiotics. “As antibiotic use has become more common, antibiotic-resistant bacteria have evolved.” This resistance due to evolution is called antibiotic resistance. It results in the antibiotics having no effect whatsoever on the bacteria, rendering the antibiotics useless. When antibiotic resistance occurs, scientists must resume their search to create a new medicine to can kill the mutant bacteria.

AIM – The aim of the experiment is to determine the relative effectiveness of several anti-microbial substances on developing pathogens. (E. coli)

Antibiotics are inarguably one of the greatest advances in medical science of the past century. Although the first natural antibiotic Penicillin was not discovered until 1928 by Scottish biologist Alexander Flemming, evidence exists that certain plant and mold growths were used to treat infections in ancient Egypt, ancient India, and classical Greece (Forrest, 1982). In our modern world with the advent of synthetic chemistry synthetic antibiotics like Erithromycin and its derivative Azithromycin have been developed. Antibiotics have many uses including the treatment of bacterial and protozoan infection, in surgical operations and prophylactically to prevent the development of an infection. Through these applications, antibiotics have saved countless lives across the world and radically altered the field of medicine. Though a wonderful and potentially lifesaving tool, antibiotic use is not without its disadvantages. Mankind has perhaps been too lax in regulation and too liberal in application of antibiotics and growing antibiotic resistance is the price we must now pay. A recent study showed that perhaps 70% of bacterial infections acquired during hospital visits in the United States are resistant to at least one class of antibiotic (Leeb, 2004). Bacteria are not helpless and their genetic capabilities have allowed them to take advantage of society’s overuse of antibiotics, allowing them to develop

The aim of this investigation was to find out which antiseptics were most effective at preventing the growth of bacteria.

E.coli outbreaks have steadily grown over the last few decades. An expansion in big farming has led to E. coli not only being found in meat, but vegetation as well, due to waste runoff. This has increased our need for adequate antibiotics that can fight bacteria, like E. coli. The best way to pinpoint which antibiotics work is by measuring their ability to create antimicrobial agents or zones of inhibition. When a paper disc that has been saturated in an antibiotic is inserted in a solution of E.coli and medium, the zone of inhibition will be noted as the clear ring that forms around the disk. The antibiotics efficacy is then determined by measuring each disk zone of inhibition, and comparing these measurements to the zone measurements of an untreated specimen. If an antibiotic is to be deemed sufficient for treating E. coli it should show a zone of inhibition that is at least double the size of the untreated specimen.

5) My hypothesis: “The higher the concentration of antiseptic, the more powerful it will be at inhibiting/ killing microorganisms which cause bacterial growth.” This theory supports my result because as you can see the zone of inhibition increases due to the increasing concentration of antiseptic. The higher concentration of antiseptic means it has been less diluted, which means its more stronger than other more diluted concentrations, this high concentration will determine how powerful the antiseptic will be at killing/inhibiting the growth of bacterium, this high concentration of antiseptic will break down the bacterial cell wall, after this process has happened the antiseptic can stop the mutation of the bacteria and inhibit the

The disinfectant that was the most effective was the Wescodyne® due to the fact that it was the only disinfectant that had a zone of inhibition for both E. coli and S. aureus, and the zone of inhibition was quite large, with it being 16 millimeters for E. coli and it being 19 millimeters for S. aureus.

It is highly recognized in the market especially among teenagers. Traditional acne products are low price range and kind of old fashioned in terms of designation and package, which catches lower income and function oriented customers. Since the main ingredients are not a secret, other popular cosmetic brands can also develop similar products. L’Oreal Launched “Acne Response” new Adult Acne Product, treats acne with gentle formula of 1% salicylic acid and 2.55% benzoyl peroxide, retail Price of $24.99 vs. $39.99 for Proactiv, which soon caught big market share and attracted some of the loose proactive customers. New producers claim that it is the ingredient which matters, not the expensive celebrity commercials. With proper marketing strategy, it won’t be hard for them to share cups from Proactiv. Other competition came from Natural skin care products. Some biological cosmetic products with aloe vera, green tea, passion flower, or Gotu kola are claimed to work well to cure acne and skin bacteria. They are natural and green with less side effect and damage to skin. However, not every company can afford the heavy input in advertising. As the first modern acne prevention and treatment product shown in the market, Proactiv will still lead the market and enjoy the competitive advantage for years.

Chloramphenicol is an antibiotic known to treat a broad range of bacteria. In this study, the effectiveness of Chloramphenicol was tested on the DH5α strain of Escherichia coli. Knowing that varying the concentrations of Chloramphenicol will result in different effects on the E. coli strain, we conducted an experiment that agar-plated the E. coli with Chloramphenicol with concentrations together. The concentrations ranged from no antibiotic to excess antibiotic in hopes to find the minimum inhibitory concentration (MIC). The study yielded the MIC of Chloramphenicol to be 85μg/mL, determining the borderline between the effectiveness and the ineffectiveness of Chloramphenicol against the DH5α strain.

To develop problem solving and experimental skills, for example, information is accurately processed and presented, experimental procedures are planned, designed and evaluated properly, producing valid results, recording results, and valid conclusion is drawn.

Considering all the benefits of oleuropein, a diet rich in the compound is definitely recommended for companion animals as well as livestock and a great candidate to be considered by Acidophil. Currently a lot of alternatives for chemotherapeutic and antibiotic substances- being used as growth promoters in livestock- are being explored due to the problems associated with them. There is an obvious risk of consuming residues antibiotic substance by humans. Also, there is an increased risk of consuming antibiotic-resistant microorganisms through products derived from animals that were given antibiotic growth promoters. Using natural products can provide the same benefits without risks while promoting weight increase and feed conversion in livestock.

According the World Health Organization (WHO), antibiotic resistance is one of the world’s greatest health threats to date (Haddox, 2013). In the article, The Health Threat of Antibiotic Resistance, Gail Haddox (2013) discusses the danger antibiotic resistance poses in today’s society and strategies to prevent the expansion of antibiotic resistance. In Europe alone, an estimated 25,000 deaths have been attributed to multi-resistant infections (Haddox, 2013). Common infections are now harder to treat due to the increased resistance to antibiotics across the world, in fact some are becoming untreatable. Antibiotics should be treated like oil, a non-renewable resource (Haddox, 2013).

Chan, 2012). Further, as some antibiotics elicit side effects and a dramatic decrease in the rate of