Osmosis And Diffusion Lab Report

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

The Osmosis and Diffusion lab was conducted to provide us with information on how built up mucus affects those conflicted by the recessive genetic disease, Cystic Fibrosis., due to a mutation to the membrane regulating chloride (Cl-). This mutation prevents the Cl- from leaving the cell causing the amount of sodium (Na+) in epithelial cells, which results in extreme mucus on the lungs and airways causing this disease to be fatal if not treated but treatment does not equate to a long lifetime. During the lab we took the data from three parts: Diffusion, Osmosis in an Elodea Cell, and finally the Role of Osmosis in Cystic Fibrosis. During Part 1 we looked at diffusion across a semipermeable membrane for starch and glucose, which resulted in both having a negative solution when placed in a semipermeable membrane. Then we looked at osmosis in the Elodea Cell to watch for the occurrence of Plasmolysis, when a cell’s plasma membrane pulls away from the cell, and how a plant cell is affected by both hypertonic and hypotonic solutions. Finally, we observed the role of Osmosis in Cystic Fibrosis using dialysis bags to represent a normal cell and a Cystic Fibrosis cell with the normal containing 1% NaCl while the Cystic Fibrosis bag contained 10% NaCl. After we ran the experiment, we looked at the Percent Change in Mass and compared them after 30 minutes. We found that Cystic Fibrosis cells didn’t change mass as much as the normal cell ending with a change in mass over -1%. The

The lab for this paper was conducted for the topic of osmosis, the movement of water from high to low concentration. Five artificial cells were created, each being filled with different concentrated solutions of sucrose. These artificial cells were placed in hypertonic, hypotonic, or isotonic solutions for a period of 90 min. Over time, the rate of osmosis was measured by calculating the weight of each artificial cell on given intervals (every 10 minutes). The resulting weights were recorded and the data was graphed. We then could draw conclusions on the lab.

The hypothesis states that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant. In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution  made during the study,  four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis.

The major objective of the experiment was to test the effect of the concentration gradient on the diffusion rate. It was hypothesized that the greater the stronger the concentration gradient, the faster the rate of diffusion would be. To test this, dialysis tubes were submerged in different concentration fructose solutions. We weighed the tubes at specific time intervals to measure the rate of diffusion of water in each different solution. The results illustrated that increased concentration gradient increases the rate of diffusion of water in the tubes. We concluded that as concentration of the

Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is

Diffusion is the transfer of molecules from an area that has a higher concentration to an area that has a lower concentration. Osmosis is the diffusion of water. The purpose of this experiment was to study the process of osmosis. In order to test osmosis, eggs that had been soaking in vinegar were taken and placed in four beakers of solution with different levels of glucose. Using this experiment we were able to determine the rate of osmosis of different solutions, with various amounts of glucose, through eggs. In the results of this lab it was found that the eggs were either hypertonic or hypotonic and that the

Group 1 received 107% for 0.0m, 109% for 0.25m, 112% for 0.5m, and 126% for 0.75m. Group 2 received 100% for 0.0m, 107% for 0.25m, 116% for 0.5m, and 117% for 0.75m. Group 3 received 100% for 0.0m, 106% for 0.25m, 114% for 0.5 m, and 117% for 0.75m. Group 4 received 100% for 0.0m, 106% for 0.25m, 113% for 0.5m, and 116% for 0.75m. Group 5 received 98% for 0.0m, 105% for 0.25m, 110% for 0.5m, and 115% for 0.75m. Group 6 received 104% for 0.0m, 108% for 0.25m, 111% for 0.5m, and 104% for 0.75m. The trend line that occurred for the rate of osmosis was an upward increase as the solute concentration increased.

The purpose of this lab is to understand diffusion and osmosis by using deshelled chicken eggs. Before obtaining the experiment, we must first understand and define diffusion and osmosis. Both diffusion and osmosis are passive transport processes meaning they do not require any input of energy. Diffusion is the movement of molecules or atoms from an area of high concentration to an area of low concentration along its concentration gradient (solute). Osmosis is the movement of water (solvent) across a selectivity permeable membrane. While diffusion moves from an area of high concentration to an area of low concentration and doesn’t need water to move down a concentration gradient osmosis moves both down and across the concentration

Cells are always in motion, energy of motion known as kinetic energy. This kinetic energy causes the membranes in motion to bump into each other, causing the membranes to move in another direction – a direction from a higher concentration of the solution to a lower one. Membranes moving around leads to diffusion and osmosis. Diffusion is the random movement of molecules from an area of higher concentration to an area of lower concentration, until they are equally distributed (Mader & Windelspecht, 2012, p. 50). Cells have a plasma membrane that separates the internal cell from the exterior environment. The plasma membrane is selectively permeable which allows certain solvents to pass through

Osmosis is a special type of diffusion. It is the diffusion of water across a semipermeable membrane which is a membrane that is freely permeable to water but is not freely permeable to solutes, the water moves from a dilute solution to a more concentrated solution (Karp, 2010). Both diffusion and osmosis are passive transport, energy is not used in the transport. In osmosis water moves across a membrane toward the solution of greater concentration, because the concentration of water is lower there (Martini and Bartholomew., 2007).

Within every cell, a movement of a solvent occurs through a semipermeable membrane to equalize the concentration of solute on both sides of the membrane. The diffusion of water across the cell’s membrane down to its concentration gradient is called osmosis. In this case, the concentration gradient is the difference of density between one side of the cell membrane to the other. Since the cell’s membrane is permeable, particles can flow freely in and out of the cell, but the net flow will be strong in the direction of lower concentration until the system has reached a stage of equilibrium, the point at which both sides of the membrane are equal. In the

This experiment was used to examine the hypothesis that: Osmosis is dependent on the concentrations of the substances involved.

Osmosis is when water passes through a cell membrane, it is also form of a diffusion, which is a form of passive transport. Osmosis will continue to until an equilibrium is reached which is when the solutions are isotonic. This means that the solution has the same amount of solute on both sides. If the solution is hypertonic, it has more solute in the solution. In this situation water will move towards it. if the solution is hypotonic, it has less solute in the solution. Whereas in this situation, water will move out of the solution.

The movement of water molecules across a semi-permeable membrane is the process of osmosis. If there is a solute and a solvent, each containing different concentration levels, then the water would move along its concentration gradient until each side of the membrane are equal. The water moves because the membrane is impermeable to the solute and the solute concentrations may differ on either side of the membrane. Water molecules may move in and out of the cell, but there is no net diffusion of water. Water will move in one direction or the other, and this is determined by the solute or solvents concentration levels. If the two solutions are of equal concentrations, they will be isotonic. If the concentrations are unequal, the