Osmosis Egg Lab Report

What will happen to the egg when a student tests osmosis with 3 different soultions in a cup.The student is trying to see or find the effects of osmosis on an egg.Osmosis is the movement of water molecules through a selecfively permeable membrane. Diffuision is when something spreads more widely and equilibrium means when somethings is at rest due to equal opposite forces. The student wants to find out if the egg will shrink, fill up, or change in any way.

The goal of this experiment was to find out how an shell-less egg’s mass and volume changed when it was placed first in corn syrup (hypertonic) and then in water (hypotonic) and why. The hypothesis was that when the egg was placed in corn syrup, it would lose mass and shrink, but when it was placed back in water, it would gain mass and swell up. The independent variable was the different tonicity of solutions that the egg was placed in; the dependent variable was the mass and volume of the egg. A pickled egg without shell was rinsed, weighed, and its dimension was measured. Then, it was placed in a cup and soaked in corn syrup.

In the rubber egg lab also known as the osmosis lab, I first measured the circumference of the egg which was 6 inches. The egg had no cracks and was hard. I placed the egg in the vinegar and within seconds the egg started to bubble. These bubbles were carbon dioxide. After 72 hours the shell of the egg had started to dissolve or flake. This would be considered passive transport as the vinegar diffused across the egg shell without any force. Seventy-two hours into the experiment the membrane was exposed, and had a circumference of 8 inches. Before the egg had a shell and you couldn’t see through it but once the vinegar acted as an acetic acid it broke down the shell and left a yellow membrane that felt like rubber this is an example of diffusion. I then placed the egg in another container and put corn syrup over the egg for 24 hours. The egg had shriveled and shrunk, the water had left the egg and went into the syrup and that is what caused the egg to shrink. The corn syrup is essentially pure sugar with very little water so the osmotic pressure is very low. I then placed the shriveled egg in water and waited for another 24 hours. After observation the egg had no

More specifically for the experiment we will need four deshelled eggs, four beakers and distilled water with a certain percentage of sucrose, a timer, and a scale to weigh the eggs. Using the process of osmosis, we will determine the weight of the eggs after being soaked in the solution. The four beakers each had distilled water in them with sucrose but all contained different amounts. One beaker contained 0% sucrose. The other beakers contained 10%, 40%, and an “unknown” amount of sucrose. Before placing the eggs into the solution, we are to weigh each egg separately to the nearest 0.1g and record this in table. Once that step has been completed, we then place one egg in each of the beakers separately that was marked with the amounts of sucrose. At fifteen-minute intervals, remove the deshelled egg from the water inside the beaker and wipe off all the excess water. After that process has been completed, we will weigh each egg separately to see if they have gained or lost their mass/weight. Once each egg has been weighed accurately, the recorded data is placed in the table provided. This step goes on for an hour. The deshelled eggs in this experiment will increase in mass in the different types of distilled water. This lab session provided the class with the evidence that diffusion and osmosis occurs within the different types of solutions.

As we know that osmosis means diffusion or dispersion of water through a selectively permeable membranes from high concentration to a lower concentration and there are many different factors that can affect the rate of Osmosis such as temperature particle size and the size of concentration ingredient. As we know that higher temperature can cause osmosis to occur at much faster rate because a molecule are more likely to pass through the selectively permeable membrane quicker than they would at a lower temperature. In this experiment the sucrose molecule was too large to pass through the dialysis bag, while water molecule able to move freely in and out due to their small size. Lastly the congregation gradient can greatly affect the rate of osmosis

The Osmosis and Diffusion lab was conducted to provide us with information on how built up mucus affects those conflicted by the recessive genetic disease, Cystic Fibrosis., due to a mutation to the membrane regulating chloride (Cl-). This mutation prevents the Cl- from leaving the cell causing the amount of sodium (Na+) in epithelial cells, which results in extreme mucus on the lungs and airways causing this disease to be fatal if not treated but treatment does not equate to a long lifetime. During the lab we took the data from three parts: Diffusion, Osmosis in an Elodea Cell, and finally the Role of Osmosis in Cystic Fibrosis. During Part 1 we looked at diffusion across a semipermeable membrane for starch and glucose, which resulted in both having a negative solution when placed in a semipermeable membrane. Then we looked at osmosis in the Elodea Cell to watch for the occurrence of Plasmolysis, when a cell’s plasma membrane pulls away from the cell, and how a plant cell is affected by both hypertonic and hypotonic solutions. Finally, we observed the role of Osmosis in Cystic Fibrosis using dialysis bags to represent a normal cell and a Cystic Fibrosis cell with the normal containing 1% NaCl while the Cystic Fibrosis bag contained 10% NaCl. After we ran the experiment, we looked at the Percent Change in Mass and compared them after 30 minutes. We found that Cystic Fibrosis cells didn’t change mass as much as the normal cell ending with a change in mass over -1%. The

The purpose to this experiment was to study the effect of osmosis in de-shelled chicken eggs in different percentages of sucrose solutions. Osmosis is the process, in which, water moves across a differentially permeable membrane. The eggs were soaked in vinegar to remove the outside hard shell but still leave the egg in its membrane. By placing the six de-shelled in different sucrose solutions, we tested the rate of osmosis. The eggs were placed in the solutions for an hour and weighed in fifteen minute intervals. Then, each weight was recorded and graphed. The results showed that the egg in the water solution gained the most weigh and the only other egg that gained a little weight was the one in the 10% solution. All the other eggs in the different solutions lost weight, even the unknown solution. According to the results the egg that was in the distilled water solution gained weight because it is the hypertonic solution. All the other eggs lost weight because they were placed in hypotonic solutions with different concentrations of sucrose. The egg that was placed in the higher concentration of sucrose lost the most weight. So, the higher the concentration of sucrose, the more water the egg lost.

This is the final step of the experiment. The shell-less egg was now placed into a new cup, but very carefully, as the egg was more fragile than when it was soaked in vinegar. The egg in the new cup was then covered with water. The egg sat in the water for a 24 hour time period once again. During this 24 hour time period the eggs appearance and size were once again recorded. After the time period had completed, the egg was removed from the water and was very carefully patted dry. Since the egg was now swollen, extra care was needed when holding the egg. The swollen egg was placed on the scale on last time and its mass was recorded as M3.

The osmolarity of a solution is the concentration measured of a solute. To determine the concentration of a solution, one must first understand what osmosis is and how it works. Osmosis is the spontaneous passage or diffusion of water or other solvents through a semipermeable membrane according to the Britannica Encyclopedia. The semipermeable membrane is one that blocks the passages of dissolved substances such as solutes. This process, in more recent times, provides a more accurate study of how water can diffuse across a cell membrane when water molecules have a high concentration to an area in which they have a low concentration. It was originally studied in 1877 by plant physiologist, Wilhelm, Pfeffer. (Britannica Encyclopedia 2015).

The eggs will increase and decrease in mass when placed solutions with different amount of solutes. This

Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is

Table 1, shows the average change in mass and average rate of osmosis of the egg after it had been soaking for ten minutes in the distilled water, 1%, 2%, 5% and 10% Sodium Chloride solutions. The table also shows the SEM for each of the averages. All of the averages show that as the salt concentration increases from 0% to 2%, the rate of change in mass decreases. From the 5% salt concentration to 10%, the rate of change in mass begins to increase. This indicates that somewhere between 2% and 5%, an isotonic environment was reached. This is all shown in the results, as the average rate of osmosis in the egg after being submerged in the distilled water is 0.105 g/min, the average rate of osmosis after being submerged in the 1% solution is

Osmosis took place in the egg under a variety of conditions because the membrane of the egg has tiny openings where water can pass through from high to low concentrations. On day one, it was a hypotonic solution because the higher concentration of water in the vinegar moved to the smaller concentration in the egg. This means the egg was filled with water, which resulted in the eggs increase in size. On day three, the hypotonic solution occurred because the corn syrup had a lower concentration of water than the egg. That means the egg released water into the corn syrup, which resulted in the eggs decrease in size. On day four, the egg was placed in water which resulted in an isotonic solution where the concentrations become even. This

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

Abstract: Different environments have different effects on the mass of an egg. This was tested by placing the egg in vinegar, corn syrup, and tap water. The vinegar increased the mass, the corn syrup decreased the mass, and the tap water increased the mass. This is due to the different concentrations of each substance and the process of osmosis. Particles of higher concentration move to regions of lower concentration.