Osmosis in a Decalcified Egg to Determine the Glucose Content of an Egg
Tovah King
C62
7/5/2017
Abstract: Diffusion is the transfer of molecules from an area that has a higher concentration to an area that has a lower concentration. Osmosis is the diffusion of water. The purpose of this experiment was to study the process of osmosis. In order to test osmosis, eggs that had been soaking in vinegar were taken and placed in four beakers of solution with different levels of glucose. Using this experiment we were able to determine the rate of osmosis of different solutions, with various amounts of glucose, through eggs. In the results of this lab it was found that the eggs were either hypertonic or hypotonic and that the
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The eggs were each individually placed in one of the beakers and were taken out and weighed every 15 minutes for 60 minutes. Weighing the eggs helped to test the process of osmosis by finding out the mass before and after soaking in water, this will dictate whether water has moved in or out of the egg.
Materials and Methods Procedure is found on pages 235 to 236 in the BIOL 110 Lab Manual.
Results
Four eggs that were previously soaked in vinegar were placed into four beakers with different levels of a glucose solution. The four beakers were filled with distilled water, 0.5M glucose, 1.5M glucose and 2.0M glucose. After the eggs were placed in the solutions, they were left for 60 minutes but weighed every 15 minutes to record whether there was an increase or a decrease in mass. The various masses were recorded as well as the percent change in mass. These were then recorded as line graphs. From this it was able to be determined if the egg had been in a hypotonic, hypertonic or isotonic solution, thus being able to determine if osmosis had occurred.
Conclusion When the 4 eggs were weighed it was found that out of the 4 solutions, 2 were hypotonic and 2 were hypertonic. The mass of 2 of the eggs increased and the other 2 decreased after 60 minutes in the various solutions. Out of the 4, the most hypotonic solution was the distilled water; and the most hypertonic solution was the solution with 2.0M glucose. The
In the rubber egg lab also known as the osmosis lab, I first measured the circumference of the egg which was 6 inches. The egg had no cracks and was hard. I placed the egg in the vinegar and within seconds the egg started to bubble. These bubbles were carbon dioxide. After 72 hours the shell of the egg had started to dissolve or flake. This would be considered passive transport as the vinegar diffused across the egg shell without any force. Seventy-two hours into the experiment the membrane was exposed, and had a circumference of 8 inches. Before the egg had a shell and you couldn’t see through it but once the vinegar acted as an acetic acid it broke down the shell and left a yellow membrane that felt like rubber this is an example of diffusion. I then placed the egg in another container and put corn syrup over the egg for 24 hours. The egg had shriveled and shrunk, the water had left the egg and went into the syrup and that is what caused the egg to shrink. The corn syrup is essentially pure sugar with very little water so the osmotic pressure is very low. I then placed the shriveled egg in water and waited for another 24 hours. After observation the egg had no
The solutions are 0% sucrose, 10% sucrose, 20% sucrose, 30% sucrose, 40% sucrose and an unknown sucrose concentration. We then weighed each egg separately to the nearest gram in order to have an initial starting weight to compare to the results throughout our experiment being conducted. The eggs were then placed in each beaker for 12 intervals at a time. After every 12 minutes the eggs were taken out and weighed to see if the weight of the egg changed. With a total of five intervals (12, 24, 36, 48, 60) the steps were repeated till the egg had reached the total time of 60 minutes. The changes in weight of the eggs were then added into a data table showing the weight of the chicken eggs in grams vs. the time in minutes. In a second data table the weight changes (g) vs. time (min.) between the eggs were taken and used the difference from each time and subtracted it from the initial
Osmosis took place in the egg under a variety of conditions because the membrane of the egg has tiny openings where water can pass through from high to low concentrations. On day one, it was a hypotonic solution because the higher concentration of water in the vinegar moved to the smaller concentration in the egg. This means the egg was filled with water, which resulted in the eggs increase in size. On day three, the hypotonic solution occurred because the corn syrup had a lower concentration of water than the egg. That means the egg released water into the corn syrup, which resulted in the eggs decrease in size. On day four, the egg was placed in water which resulted in an isotonic solution where the concentrations become even. This
In all living organisms, water and nutrients make their way to tissues and cells because of osmosis, which for all intents and purposes is essential to the survival of cells (Rao & Kaur, 2007). Besides, cells and by extension organisms also get rid of their waste products by osmosis. In animals, for instance, this process occurs in renal tubules (kidneys) that filter and clean the blood to get rid of toxic metabolites and other chemicals. In order to understand how cells acquire and get of nutrients, it is imperative to know how osmosis operates. The objective of this experiment is, therefore, to study six decalcified eggs to understand and demonstrate the process of osmosis between the inside of the egg’s membrane and multiple environments of water/solute (solutions) of different concentrations: deionized water, 20% sucrose, and 40% sucrose.
Osmosis is a natural occurrence constantly happening within the cells of all living things. For osmosis to occur, water molecules must move across a semipermeable membrane from an area of low concentration to an are of high concentration. In order to understand osmosis, people must understand the different types of concentrations that can be present within solution. One of them is an Isotonic solution where the concentration of dissolved particles is equal to that of a cell’s. Another is a hypertonic solution where there is a higher concentration of dissolved particles then inside the cell. And lastly there is a hypotonic solution where there are less dissolved particles than inside the cell. As dissolved particles move to a region of lower concentration, water moves the opposite direction as a result of there being less water in the highly concentrated region. In this experiment, gummy bears were placed in salt water, sugar water, and tap water to find the measure of osmosis between the solution and gummy bear.
The osmolarity of a solution is the concentration measured of a solute. To determine the concentration of a solution, one must first understand what osmosis is and how it works. Osmosis is the spontaneous passage or diffusion of water or other solvents through a semipermeable membrane according to the Britannica Encyclopedia. The semipermeable membrane is one that blocks the passages of dissolved substances such as solutes. This process, in more recent times, provides a more accurate study of how water can diffuse across a cell membrane when water molecules have a high concentration to an area in which they have a low concentration. It was originally studied in 1877 by plant physiologist, Wilhelm, Pfeffer. (Britannica Encyclopedia 2015).
What will happen to the egg when a student tests osmosis with 3 different soultions in a cup.The student is trying to see or find the effects of osmosis on an egg.Osmosis is the movement of water molecules through a selecfively permeable membrane. Diffuision is when something spreads more widely and equilibrium means when somethings is at rest due to equal opposite forces. The student wants to find out if the egg will shrink, fill up, or change in any way.
The purpose to this experiment was to study the effect of osmosis in de-shelled chicken eggs in different percentages of sucrose solutions. Osmosis is the process, in which, water moves across a differentially permeable membrane. The eggs were soaked in vinegar to remove the outside hard shell but still leave the egg in its membrane. By placing the six de-shelled in different sucrose solutions, we tested the rate of osmosis. The eggs were placed in the solutions for an hour and weighed in fifteen minute intervals. Then, each weight was recorded and graphed. The results showed that the egg in the water solution gained the most weigh and the only other egg that gained a little weight was the one in the 10% solution. All the other eggs in the different solutions lost weight, even the unknown solution. According to the results the egg that was in the distilled water solution gained weight because it is the hypertonic solution. All the other eggs lost weight because they were placed in hypotonic solutions with different concentrations of sucrose. The egg that was placed in the higher concentration of sucrose lost the most weight. So, the higher the concentration of sucrose, the more water the egg lost.
Osmosis is the passive movement of water from an area of low solute concentration to an area of high solute concentration, normally across a membrane which prevents the movement of solvent. This is a process by which materials may move into, out of, or within cells. Osmosis doesn’t depend on energy provided by living organisms but is affected by the properties of the cell membrane. The rate of osmosis is dependent on such factors as temperature, pressure, molecular properties such as size and mass, and the concentration gradient. In osmosis, the relationship between a solute’s concentration outside of cell and inside of a cell is described in terms of the tonicity of the solution outside of the cell. A cell is in a hypotonic solution when the solute is more concentrated inside the cell and therefore water moves into the cell. In this solution the cell swells as water enters, this may continue until it ruptures or hemolyzes. In the reverse condition, the cell is in a hypertonic solution
The lid was then placed on the jar and an observation was made and recorded. Observations of the egg were then made after 12 hours and 24 hours had passed and the observations were recorded. After 24 hours had passed, the egg was removed from the syrup and rinsed with tap water and observations were made and recorded.
An egg has a semi-permeable membrane, thus processes like osmosis could occur. Osmosis is the movement of water molecules from an area of higher water concentration to an area of a lower water concentration. Osmosis is important, especially for living organisms, as they help distribute nutrients in the body. An egg’s mass would change when it is soaked in different substances. The goal of this experiment was to investigate how an egg changes through osmosis. This experiment was done to show how substances affect the mass of an egg. To start off, an egg’s mass was measured by putting the egg in a beaker then placing it on a mass scale. The beaker was then filled with vinegar and left alone for twenty-four hours. After a day, the egg was taken
Diffusion and Osmosis Experiment with a Shell-Less Egg After Three days of Testing Methods with Water and Corn Syrup
In our lab, we investigated the effect that different concentrations of corn syrup had on the weight of an egg. It is important to note that prior research needed to be conducted to establish a hypothesis. According to /////////////, the cell membrane, which can be found in plant and animal cells, isolates the cell from the external environment while simultaneously regulating the flow of substances into and out of the cell. Being selectively permeable, the cell membrane ensures that equilibrium is reached between the cell’s external and internal environment. One way of regulating the movement of substances is through the use of passive transport. Requiring no energy, passive transport moves substances down the cell membranes concentration gradient;
In conclusion, the data that was collected helped tell us that the solutions greatly affected the cell of the egg because it caused the egg’s mass to increase than decrease. Also saw how the solutions played a role in the phospholipid bilayer and saw how it altered the shape of the egg. However, it does not support my hypothesis on the affect the egg would undergo onced placed in sugar and water but sugar was not used in this experiment, but water was used and it didn’t affect the shape of the egg, all it did was cause the egg to be in a isotonic state. According to Khan Academy (2017), states that a cell is isotonic “When the extracellular fluid has the same osmolarity as the cell, there will be no net movement in or out of the cell”. Which
Cells in all living things have an outer layer known as the cell membrane. The structure of the cell membrane consists of the phospholipid bilayer organized by the arrangement of hydrophilic heads and hydrophobic tails. It is a selectively permeable membrane, where it divides the outer environment from the interior of the cell. It can control substances moving in and out of living cells. Certain molecules like gases, water, and food are permitted to pass the membrane through the method of diffusion. Diffusion refers to the process in which molecules move on the concentration gradient, where they move from an area of high concentration to an area of low concentration. A type of diffusion is known as osmosis. It is the diffusion of water moving across the selectively permeable membrane. In this lab, students will be using eggs to construct an experiment to get a better study on how osmosis works in a cell. The eggs will be soaked in vinegar solution to remove their shells to expose each inner layer that resembles a selectively permeable membrane. The egg shell is composed of calcium carbonate that would dissolves in acidic solution such as vinegar. In the chemical reaction, it releases carbon dioxide gas. After the removal of the egg shell, it will be ready to be able to construct the experiment.