Osmosis Lab Report

Figure 1: Percentage change in potato tuber mass vs. sucrose concentration. The percent change in mass decreased as the sucrose concentration increased. Relative osmotic concentration was measured as the percent change in mass of sucrose concentration over one hour.

7. Explain how incubation plant tissues in a series of dilutions of sucrose can give an

My prediction is that as the concentration increases, the potato cell will lose more weight. This is because of the osmosis of water particles from the potato cell cytoplasm to the solution, resulting in a loss of weight. As the concentration decreases, the potato will lose less weight until a certain point where the osmosis of particles in and out of the potato cells will be equal. I also predict that as the salt

2) When the concentration was at 0.3M, the potato’s cytoplasm and the sucrose solution was isotonic. The concentration of the potato’s cytoplasm was having the same solute concentration as the surroundings. Therefore, there would be no net movement of materials happening.

Though the theory following the hypothesis is correct and the experiment was carried out with as much attention as possible in a high school laboratory, the results obtained were still indicative of a few errors and did not support the predicted hypothesis. From the results obtained it can be concluded that as the concentration of sucrose increases the average percentage change in mass decreases. This is because the salt concentration inside the potato cubes of 10%, 15% and 20% concentration is less that in the salt concentration on the sucrose solution, thus the three cubes submerged in the 10% - 20% concentrated solution lost mass (hypertonic). However the cube submerged

However one beaker received 100 mL of Deionized water with a molarity of 0.0. Afterwards a cork borer was pushed through the potato and was twisted back and forth. Once the borer was filled it was removed from the potato. Pushing the potato cylinder out of the borer, this this step was repeated six more times in order to get seven undamaged potato cylinders. Using a sharp razor blade, the potato cylinders were both cut to a uniform length of about 5cm, and were removed of their potato skins. The potato pieces were also cut in half to give the cells a greater surface area in which it was easier to absorb the solution. After the cylinders were weighed on a balance and the data was recorded in Table 4. Using the razor blade each potato was cut lengthwise into two long halves. Then the potato pieces were transferred to the water beaker and the time they were submerged was recorded. This step was repeated for all potato cylinders in which the pieces were placed in solutions 0.1 to 0.6 M. The potatoes were incubated for ninety minutes. At the end of the incubation period the time was recorded. Then the potato piece was removed form the first sample. Next potato pieces were weighed the and the final weight was recorded in Table 4. This procedure was repeated until all samples had been weighed and recorded in the chronological order they were initially placed in the test solution. Afterwards the table was completed by recording the

Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is

The class data that was received supported the group members’ hypothesis that the rate of osmosis will increase if the sucrose concentration increases as well. The trend line that was received from the data also supported the hypothesis because it increased as the sucrose concentration increased. The initial weight gain percents were all different because all of the groups’ sugar concentrations were not completely identical. The method used wasn’t entirely accurate because there could’ve been some flaws such as some bags weren’t secured enough and might have leaked sucrose into the de-ionized water causing the results to be different. The bags might have not been washed thoroughly which could have ultimately allowed sucrose into the de-ionized water. The results could have been more accurate if the baggies were left in the

In conclusion when the molarity level was at 0 and at .2 the potatoes had gained mass so therefore they were placed in a hypotonic environments. When the molarity level was .4 and above the potatoes loss mass so therefore they were placed in hypertonic environments. So the different in concentrations does change the mass of the potatoes because they determine the osmosis environments.

Data: Effect of Solute Concentration on Osmosis in Potato Cells (for the 6 groups of our class)

As we can see in Figure’s 1.2 and 1.3, when there was no sucrose solution, the potato increased in weight. This is due to the fact that the sucrose solution was hypertonic in comparison to the potato slice. Through osmosis, the solution moved along the concentration gradient and into the potato slice making it hypotonic. When there were higher concentrations of sucrose solution, the potato decreased in weight. This is due to the fact that the potato was hypertonic in comparison to the potato. Through osmosis, sucrose from the potato moved along the concentration gradient out and into

Small slices of potato are placed in six concentrations of sucrose: 0.0 M, 0.1 M, 0.2 M, 0.3 M, 0.4 M, and 0.5 M. The initial mass of potato is noted before being placed in the solution. The potato remained in the solution for one hour then the potato was removed from the solution and dried off before the final mass was noted. The null hypothesis is that the solutions of different molarities of sucrose will not affect the

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

How do different concentrations of sucrose solutions (0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M) affect the mass of potato cores when in sucrose solution?

out the potato and dry it to ensure excess water is not added to the