PCQL Lab Report

All standardizations are performed in triplicate. Weigh out .1000-.1200 gram KIO3. Add 70-80 mL of deionized water. Swirl and dissolve. Add 3 mL of 6M HCl. Swirl and mix. Quickly titrated the brow-red solution with 0.1M Na2S2O3 until it is light yellow. Then add 3.5 mL of starch indicator. Titrate again until the dark color first disappears.

In Figure 2, the lower meniscus lies below the 3 mL line and 4 minor gradations that signify 0.1 values. Estimating the uncertain digit to be 0.04 mL, the reading we record for this particular buret is 3.44 mL.

1. To titrate a hydrochloric acid solution of “unknown” concentration with standardized 0.5M sodium hydroxide.

10 microliters of the sample is then added and the assay absorption is measured at 340nm. If absorbance was above 1.5, samples were diluted.

1. Are any of the lab values in Table 1 out of normal range? Do you see some that are too high or too

0.1 gram of my product from the second trial was weighed in a tray and was then added to a fourth test tube containing 2.0 mL of Iron (III) chloride, which was measured using a 10 mL graduated cylinder, to test for

Purpose: To use indicators to test for the presence of organic compounds in certain substances.

The purpose of this lab was for the student to get involved with his or hers new lab kit as well as being able to know, identify and use each other tools provided in the kit. Another key learning aspect of this lab is to teach the student how to measure properly the many units in the SI system. I will be using laboratory dilutions, measurements, and weights to then calculate using algebraic formula.

must be above 8.5 and below 6.5. If the pH is too acidic, then the

During a titration the pH of the solution will be monitored using a pH meter from that we get a titration curve. The titration curve is then used to determine the equivalent molecular weight and Ka value of the unknown weak acid, from that we are

Reporting of the method used to evaluate LV mass is crucial to specify what cut off values were used, and to expect the change in sensitivity and specificity, and the cut off values if a change in the method used to detect LV mass occurred during follow up periods.

I will use this because it is already made up by the school. Furthermore it will limit risks as it is diluted sodium hydroxide.

(Lee, J. W., et al. (2006). "Fit-for-purpose method development and validation for successful biomarker measurement." Pharm Res 23(2): 312-328.)

Assay of curcumin = (absorbance of sample / absorbance of standard × concentration of standard / concentration of sample) 100

For generating valuable data with a desired accuracy and to quantify concentration of the constituents present in the samples being analyzed.