Paper Chromatography Lab

Major scientific concepts in this lab include chromatography and pigments. Chromatography is the division of mixtures, solution, and others into its different components. Chromatography is often used to separate a solution into its separate parts. Chromatography filters complicated compounds accurately without involving a difficult process so it is often used. In this lab, we used chromatography to separate the different pigments in the plants. When looking at plants, the colors of the plants are the colors that are reflected from the different wavelengths of the light spectrum. The colors that are not evident are the colors the plants absorb and use as energy. Pigments are the compounds of a plant the enable the reflected colors to be evident. These pigments function to capture light energy from the sun to enable the plants to undergo photosynthesis.

Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct?

III. Hypothesis: If I stack quarters on a wet paper towel, then Bounty will be able to hold the most amount of quarters without ripping.

Thin Layer Chromatography (TLC) works in relation to the polarity of chemicals. A plate is first covered with aluminum foil or silica etc. and has solutions of varying polarities placed upon the aluminum foil or silica. When placed in a in a puddle of solvent that moves up the plate, the different inks i.e. the solution will move up the place based on their Rf values. Adherence increases with increasing polarity, so the less polar compounds will be carried farther by the solvent. Eventually the dyes will separate into their compontnets, which can be visibly seen. This is then used to determine who the ink of the unsigned note belonged to along with the pen that it belonged

Chromatography is a fairly simple process. First, you put a dot of ink(or in our case, the M&M food dye) near the bottom of some chromotography paper (also known as filter paper), and then hang the paper vertically with its lower edge (the one closest to the spot of dye) dipped in a solvent (In our case, the sodium chloride solution). Capillary action forces the solvent to travel up the paper, where it meets and dissolves the ink. The dissolved ink (which is the mobile phase) slowly travels up the paper (the stationary phase) and separates out into its different elements. Another way of describing it is to think of the liquid as an adhesive-like liquids, some of which stick more to the solid and can travel more slowly than others. This is

The experiment began by undergoing a hand washing with antibacterial soap for a minimum of 90 seconds and thoroughly drying them. Afterward, six (6) Bolder paper towels were taken and torn at the perforated line. An individual towel was then folded 3 times [See figure one (1)]; This step was repeated five (5) more times. Then sixty (60) pea seeds were counted out and separated into ten (10) equal groups. The pea seeds when observed had a shrivels like coat with a dull army green color. Next was to measure the length of the sixty (60) seeds and then calculate the average by adding up all the measurements then dividing the sum by sixty (60); this was recorded in a table provided [See figure two (2)]. Then the folded paper towel was taken and one group (10) of pea seeds

The chromatography paper used for each of the beakers would have to be the same because different chromatography papers may contain different amounts of cellulose, which would vary how attracted the solvent is to the paper. The amount of time allowed for the dyes to travel also has to be the same for each solvent in order to obtain accurate Rf values for comparison. Conclusion: Molecules that are attracted to one another are best separated by water (solvent). The Rf values were 0.813

Abstract. Thin Layer Chromatography is a simple procedure that allows you to determine how many and what kind of compounds are in a mixture. By understanding the properties of the TLC system and how it combines with the functional groups of amino acids the retardation factor (Rf) values can be calculated and compared to specific hydropathy values of amino acids. Thin layer chromatography is used to separate and recognize a certain compound. The silica gel that is located on the outside of the TLC sheet functions as the stationary phase and the solvent mixture functions as the mobile phase.

Sugar content in organic products versus natural products tested with thin layer chromatography Carbohydrates are one of the most important components for food sources. Carbon based molecules are also called organic compounds. Large organic molecules have elaborate shapes. The chain of carbon atoms is called a carbon skeleton. Other monosaccharides carbon skeletons may have 3 to 7 carbons.

Thin layer chromatography is the separation of substances using different solvents that run up the TLC plate to find different materials and colors in the sample. Three solvents in this test were one hundred percent acetone, one hundred percent isopropyl, and distilled water. In this test we timed the speed of each solvent and recorded the best color content. PACE Introduction Thin Layer Chromatography is a commonly used experiment in forensics, and is the separation of substances using different solvents. What I want to find out is what the fastest solvent is, but keeps the best color on the TLC plate.

In this experiment, paper chromatography was used to determine what pigments were present in spinach extract. From this experiment, we can see that four different types of pigments are present in the spinach extract used, the following are those pigments: chlorophyll a, chlorophyll b, beta carotene, and xanthophyll. The absorption and reflection of these pigments all revolve around the basis of the electromagnetic spectrum. The form of electromagnetic radiation is released as light and overall it is a type of energy that travels in waves. Going back to the spectrum itself, all the different types of electromagnetic radiation combine to form the electromagnetic spectrum, which tells us which colors can be absorbed and/or reflected. Each wave

Chromatography is a separation technique in which the mixture to be separated is dissolved in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the original mixture depends on how strongly each component is attracted to the stationary phase. Substances that are attracted strongly to the stationary phase will be retarded and not move alone with the mobile phase. Weakly attracted substances will move more rapidly with the mobile phase.

The chromatogram that was received is very similar to the one in the text book. The two chromatograms show six total peaks (if the injection peak is counted) a total of five peaks (if the injection peak is not counted). A difference between the two chromatograms is that on the text book chromatogram a solvent was used to dilute the sample which shows in the chromatogram creating an extra peak (1). No solvent was used to dilute the sample of the mixture of products in the laboratory experiment. Comparing the two chromatograms by looking at them they are very similar in peak placement and heights. The injection peak represents the headspace sample (vapors above the sample) that were injected into the gas chromatography column.

Chromatography Investigation Chromatography is a highly regarded technique used to separate the components of a mixture. It is based on the principle that each component possesses a unique affinity for a stationary phase and a mobile phase. The components that are more inclined to enter the mobile phase will migrate further on the chromatogram and distinguish themselves from the other components. The type of solvent used in chromatography is known to directly affect the separation of the mixture. In this experiment, thin-layer and column chromatography will be utilized to separate the numerous chlorophyll and carotenoid pigments of a spinach extract.

Gel-Filtration Chromatography is a commonly used method used in order purify a protein from a mixture, by means of separations. Different biomolecules differ in size, or their molecular weight. In the gel matrix inside the chromatography column, there are gel beads which are porous to allow certain sized molecules to enter. The molecules that are able to enter the pores of the gel, are held in stationary phase and will elute from the column later on, these are usually smaller, to medium sized molecules. Larger molecules that are not able to fit in the pores will elute out of the column first, they are involved in mobile phase where they just go straight through the column without interacting with the gel beads. Smaller molecules will have a higher elution volume, while the larger molecules will have a lower elution volume. The volume to elute the protein is inversely proportional to the molecules size.