Pepper Seed Dna Extraction

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Pepper Seed DNA Extraction Biochem lab: CHE 452L marisol gomez Pepper Seed DNA Extraction Biochem lab: CHE 452L marisol gomez 2015 2015 INTRODUCTION The jalapeno is a member of the capsicum family, along with many other peppers. The usual methods for characterization of different pepper species are based on their morphological and physiological traits, however this many not always be enough. For peppers, their traits are influenced by things like their genotype or their specific environment. Genomic markers can allow for a more direct comparison of closely related individuals (Ansari and Khan, 2012). In our case we focus on DNA extraction. The two basic parts of a DNA extraction procedure include the breaking of the cell walls…show more content…
Reliable DNA analysis is also important in the management of wild life. In the case of Wedrowicz, the koala population is being threatened and the need for data on their genetic diversity is of extreme importance. However obtaining the DNA from the koala is not a simple task, this is due to cost, stress, and ethics of capturing a koala. Sometimes the only way to obtain a sample of DNA is by the collection of scats. This means that once the DNA sample is obtained it must be rushed to the lab for extraction while the sample is still fresh. Obtaining this DNA can allow for analysis of population, breeding, and habitat of not only koalas, but of many other mammals (Wedrowicz, Faye, 2013). MATERIALS AND METHODS Plant Materials Seeds from different capsicum plants were purchased locally. The pepper seeds collected were ‘Japanese chili’, ‘California Bell’, ‘Serrano’, ‘Habanero’, and ‘Jalapeno’. Chemicals and Reagents The chemical and reagents used for the extraction and quantitation of DNA were: Plant DNAzol (0.3ml/0.1g), 100% ethanol (100%: 0.225 ml/0.1 g, 75%: 0.3 ml/0.1 g), Chloroform (0.3 ml/0.1 g), Plant DNAzol-ethanol solution: Plant DNAzol, 100% ethanol (1:0.75 v/v), TE buffer (10 mM Tris, 1 mM EDTA pH 8.0), 1.2% agarose gel (Agarose, 1X TAE buffer), 6X loading buffer (glycerol, Tris/EDTA pH 8.0, ethidium bromide), .25X TAE buffer, Restriction enzymes and Restriction endonuclease buffers. All the chemicals used were quality grade. The restriction
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