Peroxidase Enzyme Lab Report

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Introduction Cells are the building blocks of life. Life itself would not be possible without cells and the actions they carry out. Hundreds of biological and chemical reactions take place in the cell every second. Most of the reactions in a cell use enzymes to speed up the reaction. An enzyme is a protein catalyst used by living organisms to increase the rate of biological reactions (Freeman et. al. 2016, p90). A catalyst brings substrates together in a precise orientation that makes reactions more likely. Enzymes have an “active site,” which is where the reactants bind to the enzyme. The active site is where catalysis occurs. The reactants of the enzyme are called the substrates. Enzymes are extremely effective at catalyzing reactions because…show more content…
Peroxidase is an enzyme that breaks down hydrogen peroxide in our cells (Bansal et. al., 2016). Peroxidase is essential in life because hydrogen peroxide can cause damage to the cell. Hydrogen peroxide is the substrate to the peroxidase enzyme, where it binds to the active site and is broken down into water. In order to monitor the breakdown of hydrogen peroxide, the colorless dye guaiacol binds to the peroxidase and becomes oxidized as hydrogen peroxide is reduced to water, which then turns brown. Hydroxylamine is an inhibitor that has a similar structure to hydrogen peroxide. When hydroxylamine binds to the active site of peroxidase, it inhibits hydrogen peroxide from binding, thus preventing the breakdown of hydrogen peroxide (Lab Handout,…show more content…
The first part of the experiment measured the effects temperature has on the enzyme activity. Our hypothesis for part one is that peroxidase activity is affected by temperature. One can predict that if the temperature of the environment around the enzyme increases, then the enzyme activity will increase. With that being said, there is an optimal temperature for peroxidase, so at some point the peroxidase will decrease in activity once it exceeds it’s optimal temperature. The second part of the experiment measured the effects of inhibition and how it influenced enzyme activity. A hypothesis for part two would be that peroxidase activity is affected by an addition of an inhibitor. If hydroxylamine is added to the reaction mixture, then the breakdown of hydrogen peroxide will decrease. Since hydroxylamine is an inhibitor, one can predict that the rate of peroxidase activity will decrease and the hydrogen peroxide concentration in the mixture will be much
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