Peroxidase Essay

1460 Words Oct 6th, 2013 6 Pages
In a 1-2 page report, address the following. The first part of your report is the Results section of a scientific paper. In this section, present the results of your experiment without any interpretation. Be sure to adequately answer the following questions:

How did the rate of the reaction change as you changed each of the three variables?

If you vary the temperature, after a certain temperature, the enzymes will become denatured and then the rate will decrease.

Each enzyme functions at an optimum pH.

Well according to the graph, you see that the enzyme did not function at all when boiled which can be explained by the fact that it got denatured. Denaturing means the secondary and tertiary structure of the enzyme
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Most enzymes become denatured past forty degrees Celsius. Also, most enzymes lose activity when frozen at about zero degrees Celsius. As stated before, enzymes have an optimum pH at which they function. Enzymes can lose all activity or we can say that the rate of activity decreases for enzymes at extreme pH values. Each enzyme functions best at a different pH. As researched, the optimum pH for peroxidase is between 6.0 and 6.5, or even ranging from 6.0 –7.0 is better. The majority of enzymes have their best activity between a pH of 6 to 8. The vast majority of enzymes are proteins. Because enzymes are proteins, they are made of amino acids causing them to weigh differently. Also, most enzymes are made up of multiple enzyme complexes. With this being so, the weight of an enzyme can affect its activity. In enzymes, there are also many types of bonds found. These bonds contain hydrogen bonds, disulfide bonds, or covalent bonds, which will affect enzyme activity. For instance, covalent bonds are the strongest and hydrogen bonds are the weakest. Along with the structure of the enzyme, stronger bonds hold the enzyme together longer under extreme conditions. Basically, the enzyme has to bind to a specific site on the substrate-or the molecule that needs to be changed. The enzyme can bind at a site called the active site, or at a different site on the substrate called an allosteric site.
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