Petri Dish Lab

If hatching viability is affected by the amount of motor oil in the environment of the brine shrimp eggs, then when the amount of motor oil is increased the hatching viability will decrease.

Firstly adult female brine shrimp ovulate approximately every 140 hours and in optimal conditions the fertilised eggs can almost immediately hatch, even over a relatively short lifetime of a year this is a lot of young. They have another brilliant reproductive adaption; while in extreme conditions, such as low oxygen level or salinity above 15‰, female brine shrimp produce eggs with a chorion coating which has a brown colour. These eggs, also known as cysts, are metabolically inactive and can remain in total stasis for two years while in dry oxygen-free conditions, even at temperatures below freezing. The shrimp have also been able to survive dangerous salinity levels as the eggs wait to hatch if the salinity levels are too high. Once placed back in briny water the eggs hatch within a few hours. This ability also has enabled them to spread the population by the eggs travelling on the feet of birds. They have also been known to reproduce asexually by parthenogenesis in the absence of

Brine Shrimp are a micro-crustacean that is found mostly in inland saltwater bodies, however, the salinity levels that they live in vary greatly. The conditions that they require can be made at home as the eggs (cysts) of the shrimp are dry, hard shells that can withstand drought and go without water for up to 50 years. This study presents the results of 3 different salinity levelled environments to the Brine shrimp in order to discover a salinity level that produces the most successful hatching rate. By filling 3 Petri dishes with 20mL’s of purified water, each dish contained varying sodium chloride amounts from 15ppt, 25ppt, and

According to Darwin and his theory on evolution, organisms are presented with nature’s challenge of environmental change. Those that possess the characteristics of adapting to such challenges are successful in leaving their genes behind and ensuring that their lineage will continue. It is natural selection, where nature can perform tiny to mass sporadic experiments on its organisms, and the results can be interesting from extinction to significant changes within a species.

The solutions are 0% sucrose, 10% sucrose, 20% sucrose, 30% sucrose, 40% sucrose and an unknown sucrose concentration. We then weighed each egg separately to the nearest gram in order to have an initial starting weight to compare to the results throughout our experiment being conducted. The eggs were then placed in each beaker for 12 intervals at a time. After every 12 minutes the eggs were taken out and weighed to see if the weight of the egg changed. With a total of five intervals (12, 24, 36, 48, 60) the steps were repeated till the egg had reached the total time of 60 minutes. The changes in weight of the eggs were then added into a data table showing the weight of the chicken eggs in grams vs. the time in minutes. In a second data table the weight changes (g) vs. time (min.) between the eggs were taken and used the difference from each time and subtracted it from the initial

To approach this problem, eggs of the brine shrimp were placed in 5 different solutions with different salinity. By observing how many eggs hatch per given number of eggs in the solution, the hatching viability was calculated for each solution.

Behavior of Brine Shrimp in Habitat Selection Introduction: This experiment was developed primarily to find the optimal or preferred living conditions of adult brine shrimp. In this lab 3 variables were tested in different degrees to determine which the shrimp prefer. The different variables were light, ph, and temperature. From outside resources I have found that brine shrimp can survive in temperatures ranging from 4° Celsius to 40° Celsius with an optimal temperature of 20°-25°. So I hope to reinforce this research by testing its validity in the lab. From another outside source brine shrimp display positive photo taxis, which means that they are attracted to light. And finally from my last outside source I have found information that

The second experiment was split into three runs each ending with a variety of results. In run one of the experiment, 38 isopods were released into the glass arena. It was determined after the eight minutes elapsed that 57.2% ran to the sucrose sponge, 8.6% ran to the ethanol sponge, 23.0% ran to the NaCl sponge and 11.1% ran to the RO water sponge. For run two, there was a significant shift in the results with 1.3% of the isopods going to the sucrose sponge, 15.0% going to the ethanol sponge, 20.0% going to the NaCl sponge, and 63.8% going to the RO water sponge.

The development of A. salina will be unfavorable if exposed to too much ethyl alcohol. Treatment two and three have more ethanol alcohol than treatment one and more brine shrimp died when put into more ethanol alcohol. Since the viability was less than the viability in treatment one, the hypothesis was supported. Treatment four was the constant and had no ethanol alcohol which less cysts died when compared to treatment two and three, but when compared to treatment one, more had died,

They were separated into three groups of four. Each group of four planaria held a different spot in the labeled petri dishes. The bodies were cut off with a blade while carefully avoiding cutting their gut, and their heads were the only piece to remain, amputating the gut or pharynx would be detrimental to the planaria (Halas et al. 1961). Each group of planaria was kept separate in different exposures to light to see if this would have any effect on their diet, movement, or regeneration. The petri dishes were filled with well water and all planaria were kept on a shrimp diet. To test different light exposure one group was placed in complete light, the second group covered with aluminum foil with holes poked in it for partial light, and the third group was in complete darkness of aluminum foil. Since planaria can grow and shrink very quickly based on amount of food they are fed every two days for two weeks their well water was cleaned out and shrimp diet remained constant (Rink 2012). This process took about twenty minutes each time, because ten minutes were allowed for them to

The ninth step was to observe the petri dishes for eight days and record what we observed growing in the petri dish over the course of those eight days. Data Results and Observation Sample Day 1 Day 4 Day 5 Day 6 Day 7 Day 8 Date April 15, 2016 April 18, 2016 April 19, 2016 April 20, 2016 April 21, 2016 April 22, 2016 Control Control shows no change Control shows a white hair like substance Control shows a white hair like substance Control shows a white hair like substance Control shows a white hair like substance Control shows a white hair like substance Keyboard Keyboard shows no change Keyboard shows a white hair like substance Keyboard shows a white hair like substance Keyboard shows a white hair like substance Keyboard shows a white hair like substance Keyboard shows a white hair like substance Mouse Mouse shows no change Mouse shows a white hair like substance Mouse shows a white hair like substance Mouse shows a white hair like substance

Offspring that possess favorable traits are more capable of surviving into adulthood and thus reproducing. We know from genetics that there is an increased likelihood that their offspring would also possess that desirable trait. The population of individuals possessing the trait would increase as more individuals possessing the trait survived to reproductive years, and fewer without the trait did not. Natural selection can help prevent a species from going extinct if they were to face adverse conditions that

The process of evolution by natural selection begins with the overproduction of offspring, continues with the variation of these offspring, then the selection of the offspring that best fit the environment, and finally, the acclimatization of these offspring to their surroundings. While these steps don’t necessarily have a concrete duration of time in which they take place, the process follows this general pattern. Overproduction is necessary to be able to select from the most “suitable” or “fitting” offspring from a sizeable group. The three or so steps following overproduction coincide with one another and are necessary for the adaptation of offspring in an environment.

The pH in the control was 8.043, and the acidified tanks pH was 7.712, both had a 0.006 standard error. Temperature remained constant at 4.1C and the salinity was 31.5 ppt. The Acidified water differed from the control having higher pCO2 and DIC. It also had lower ΩAragonite, and ΩCalcite than control water, these are the most important molecules required for calcification in the crabs exoskeleton. In the embryogenesis experiment the first three principal components explained 90% of variation in embryo morphometrics. The first PC positively correlated with yolk size and negatively with egg and eye size. The samples taken from the acidified conditions in March showed larger eggs and eyes and smaller yolks than the control conditions compared to the month of February. Differences were in months, treatments, and individuals, but not interaction between embryo and larval phases. The second PC showed all positive correlations with the exception of the maximum eye diameter; variation occurred between crabs but not months or treatments. Long et al did not describe PC three as it was not easily interpreted and had minimal input on the variation in the data; it showed differences in females and treatments, but not for months. For adult female survival testing only one acidified female successfully molted and survived 2 weeks post-molt, all four control crabs survived. One acidified female did not

Professor Weinberg load up three petri dishes with different compounds: tetracycline, bacteria, and an elemental nutrient. During his experiments, he noticed that the bacteria did not stop to growth, so he deduced that a nutrient was providing so much booster fuel, in this case iron. However, to be valid the results, first I would put on a dish too much iron, then add the bacteria and the antibiotic. As a result, I would expect that the high levels of iron lead to the highly increase of bacteria, and moreover, the antibiotic did not work at all. Consequently, I would put few iron in a dish, then add the bacteria and the antibiotic, therefore, as I expected, the low levels of iron did not permit the growth of bacteria, and the antibiotic was