Pglo Transformation Lab Report

E. coli HB101 was transformed with pGLO plasmid then grown on media containing ampicillin and/or arabinose and on medium containing neither (Brown, 2011). This is done for selection of transformed cells since not all cells are expected to take up the plasmid (Brown, 2011). We also expect roughly the same CFU on any plate(s) receiving samples from the same microcentrifuge tube, since they are getting the exact same

This experiment was designed to test and observe the transformation efficacy of the pUC18 and lux plasmids in making E. coli resistant to ampicillin. Both plasmids code for ampicillin resistance, however, the lux plasmid codes for a bioluminescence gene that is expressed if properly introduced into the bacteria’s genome. The E. coli cultures were mixed with a calcium chloride solution and then heat shocked, allowing the plasmids to enter the bacteria and assimilate into the bacterial DNA. The plasmids and the bacteria were then mixed in different test tubes and then evenly spread onto petri dishes using a bacterial spreader, heating the spreader between each sample to make sure there is no cross contamination. Each of the dishes was labeled and then incubated for a period of 24 hours. The results were rather odd because every single one of the samples grew. Several errors could have occurred here, cross contamination or possibly an error in preparation as every single sample in the class grew, meaning all samples of the bacteria transformed and became ampicillin resistant.

The plasmid pGLO contains an antibiotic-resistance gene, ampR, and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli, so if E. coli, so if E. coli cells contain the ampicillin-resistance gene, the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus, transformed E. coli cells containing ampicillin-resistance plasmids can easily be selected simply growing the bacteria in the presence of ampicillin-only the transformed cells survive. The ara control region regulates GFP expression by the addition of arabinose, so the GFP gene can be turned on and

In the pGLO Bacterial Transformation lab, Escherichia coli is transformed with a gene encoding green fluorescent protein by inserting a plasmid containing the GFP gene, beta-lactamase, and arabinose into the bacterium. Successfully transformed bacteria will grow in the presence of ampicillin and glow a bright green color under ultraviolet light. The sugar arabinose is responsible for switching on the GFP gene in the transformed cells, without it, the gene will not be expressed.

The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab, the Green Fluorescent Protein, which is typically found in the bioluminescent jellyfish Aequorea Victoria, was cloned, purified, and moved from one organism to another with the use of pGlo plasmids. It was hypothesized that if bacteria that were transformed with +pGlo plasmids are given the gene for GFP, then transformed cell colonies

How does the addition of pGLO plasmid to a solution containing E. coli bacteria affect the growth and characteristics of the bacteria? Genetic transformation is the incorporation of foreign DNA into an organism to potentially change the organism’s trait. Plasmids are small circular DNA that replicate separately from the bacterial chromosome. In nature, these plasmids can be transferred between bacteria allowing for the sharing of beneficial genes. Due to this characteristic, plasmids allow for genetic manipulation and can be moved between bacteria easily. The pGLO plasmid utilized in this experiment encodes the gene for Green Fluorescent Protein (GFP), which under the right conditions can produce a glow. The gene regulation system present in the pGLO plasmid requires

Scientists can study and manipulate genes by using precisely engineered plasmids, According to ADDGENE, a nonprofit plasmid repository, plasmids “have become possibly the most ubiquitous tools in the molecular biologist’s toolbox” (“What Is a Plasmid?”). In this experiment, we will use pGLO to genetically transform the bacteria E. coli. pGLO is a genetically engineered plasmid that carries the reporter genes for both green fluorescent proteins (GFP) and the ampicillin resistance. GFP, a protein typically found in the bioluminescent jellyfish Aequorea victoria, exhibits a bright green fluorescence in the presence of blue to UV wavelengths (Mecham); the protein absorbs UV light from the sun and emits it as a lower energy green light, exemplifying the second law of

coli, to express a gene that is normally causes jellyfish to be fluorescent. The plasmid that contains the gene for the green fluorescent protein (GFP) is called pGLO. The type of genetic transformation we used in the experiment was heat shock which made the membrane of E. coli fluid and allowed the pGLO to enter the cell. In order for the E. coli to express the GFP gene there needs to be arabinose presence, which is a sugar that acts as an on switch for the pGLO. Using this information, we predicted the bacteria E. coli will grow and express the GFP on the plate that consists of +pGLO, ampicillin, arabinose, and LB broth (Luria Bertani broth).

Introduction: We are obligated to construct a pGLO plasmid with a gene from a jellyfish that is encoded with Green Fluorescent Protein (GFP). The Green Fluorescent Protein is an antibiotic resistance gene. The bacteria throughout our experiment of the transformation of pGLO is transformed by the existence of ampicillin; which the primary inducer is to express GFP. Using the LB platelets and the LB-Amp-ARA platelets the plasmid

In two weeks of genetic transformation, we were able to successfully complete the objectives of the lab by correctly performing a transformation of bacterial cells. During the lab, we were able to successfully complete the components that were necessary for a transformation of bacterial cells. Viable cells were transformed, able to grow, and were put in a sterile environment conducive to growth. With the exception of the plate labeled LB/Amp: -pGLO, our results followed the lines of a successful experiment. The LB/Amp: -pGLO plate was contaminated and had colony growth in it. In order for the transformation to be completely successful the LB/Amp: -pGLO plate would have had zero colony growth. As stated in the laboratory manual, biotechnologists

Transformation of Escherichia coli to Form a Recombinant Plasmid Containing Genes for both Ampicillin and Kanamycin Resistance

The E. coli that was not exposed to the pGLO plasmid and was in contact with LB broth and ampicillin (amp) had no bacterial growth. There is no growth in this Petri dish because amp is a bacterial antibiotic.

In this experiment students attempt to transform E. coli with a plasmid. The plasmid that will be used to transform the bacteria in this experiment is pGal and occurs naturally in E. coli. Transformed bacteria cells will grow in the presence of the antibiotic while bacteria cells that did not take up the plasmid, will not grow. Students will use mathematical calculations to determine the efficiency of the transformation process. The transformed E. coli with the

The results obtained in the bacterial culture plates were the following: In Plate # I, the bacteria grew as a lawn of bacteria, making it uncountable. In plate # II without pGLO plasmid, there were no bacteria, all of them died in the presence of ampicillin. In plate # II with pGLO plasmid, the bacterial culture lived and grew. In plate # III with pGLO plasmid and araC protein, the bacterial culture grew and glowed. The transformation efficiency is the number of colonies on LB/amp/ara plate divided by the amount of DNA on the plate. Figure 1 shows the culture plates under visible light, figure 2 shows the culture plates under ultraviolet light, table 1 shows the characteristics of the culture plates obtained after the experiment, and Table 2 shows the calculations done for finding the transformation

This experiment was performed to test the hypothesis if LB nutrient broth, +pGLO and -pGLO Ampicillin, and Arabinose was placed in the E. coli plates, then there will be a significant growth in the newly transformed bacteria and it will possess the ability to glow under UV light. The measurements were recorded from the bent glass tube in each glass test tube. The transformation protocol tested for the newly possessed traits in E.coli bacteria. Throughout the experiment there were many probable reasons for failure. If the pipettes and sterile loop were not thrown out in between each use, a cross contamination could cause a miscalculation in the experiment causing the data results to fail. The hypothesis that was tested was validated due to the positive results with each experiment stating that newly transformed organisms due in fact pass on traits.