Pglo Transformation Lab Write Up

Better Essays
The transformation is the process in which the foreign cell DNA is hosted to hereditarily modify the cell. It is very useful tool for genetics because it enables them to introduce foreign DNA into bacteria, and useful for gene cloning in bacteria by making them carry specific gene sequences.
The DNA fragment is the blueprint for every piece of the bacteria. The information in the DNA and RNA can be copied and translated into proteins. Depending on roles the proteins move to various part of the bacteria, which transport the proteins around the cells. There are many different ways in which bacteria obtain foreign DNA, involving the procedures of transformation. In transformation, bacteria carry their DNA from the environment through their cell wall. Frederick Griffith discovers the process of transformation in 1928 while examining virulent bacteria that cause pneumonia in mice. Griffith used two strains of pneumococcus bacteria: a virulent, smooth strain (S-strain) and a non-virulent rough-strain. When mice were injected with smooth strain it killed the mice and the non- virulent rough strain, mice were healthy and alive. The mice that are exposed to r-strain cells stayed healthy and good, and those exposed to dead smooth cells stay healthy and good when r-strain
…show more content…
In +LB/amp 1:1 plate there were about 200 cells, +plasmid/variable has 150 and there was no cell growth in –LB/amp 1:1 plate. There were about 100 colonies in +LB/amp 1:10 and about 50 colonies in +plasmid/variable plate. The plate that is labeled with +LB/amp 1:100 got about 28 colonies, whereas in +plasmid and + variable plate, no growth is shown. There were approximately 9 colonies in LB/amp 1:1000 +plasmid and + variable, but no growth in 1:1000 +LB/amp plate. Also, there were no growths on both of LB/amp 1:10,000 plates (Table2). This experiment is consistent with our
Get Access