Ph and Buffer Solution

1998 Words8 Pages
Title: pH and buffer solutions Aim This experiment was carried out to determine the role of buffer solution and the factor which affect the buffer capacity. Besides, this experiment was carried out to investigate the solubility of protein casein over a range of pH concentration. This experiment also was carried out to determine the isoelectric point of the casein and the effect of the isoelectric point toward the casein solution. Methods Verification of the Henderson-Hasselbalch equation Figure 1: Method for Verification of the Henderson-Hasselbalch equation The tris (hydroxymethyl) aminomethane buffer system Figure 2: Method The tris (hydroxymethyl) aminomethane buffer system Effect of…show more content…
Question & Answers 1. The differences in pH value increases along the actual pH value. This is because the phosphate buffer has high buffering range in the pH range between 6 .Therefore the phosphate buffer with pH 6 and 7 which is more closer to the pKa value (pH 7) had a lower change in pH compared to phosphate buffer at pH 8 which moves away from the pKa. The ∆pH will increases as the direction of shift favours away from the pKa. The ΔpH for pH 7 will be the lowest because it is nearest to its pKa value. The phosphate buffer for pH 6 has the largest change in pH where it furthest from the pKa value. For tris buffer at pH 8, the change of pH was small , 0.09. This is because the direction of shift is towards pKa. 2. Temperature will affect the pH measurements due to changes in dissociation constants of the ions in the buffer solution. With the decrease in the temperature, the tendency to dissociate decreases and hence the ionized component decreases as well. (Gravlee et al., 2007). This cause the reduction in H+ ion in the buffer
Get Access