Phenyl Red Lab Report

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

1. We measured 2 mL of diluted hydrogen peroxide (the substrate), 1 mL of guaiacol (the product indicator), and 1 mL of neutral buffer (pH 7) with a syringe and disposed it into tubes 1, 2 , 4, 9, 11, and 12.

The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).

In this experiment, an alkyne—diphenylacetylene—was prepared by a double dehydrobromination. Potassium hydroxide (KOH) and high heat were used to accomplish this. The precipitate of this reaction was collected by vacuum filtration. It was then washed with water and recrystallized using ethanol. The product was then isolated again before the weight was taken for further analysis of percent yield. The melting point was also taken to identify that it was the desired product.

Figure 2 is a representation of the average saturation of each cuvette at a specific point time as a function. The y-axis shows the specific saturation points from figure 1, and the x-axis provides the different levels of pH. The pH scale provided on the x-axis ranges from 0 to 14, 0 being the most acidic and 14 being the most basic. The point chosen from figure 1 was the saturation levels of each cuvette at 110 seconds. The saturation point was chosen because in the previous graph at time 110 seconds the reactions of

The goal of this was to successfully accomplish the synthesis of para-Chlorophenoxyacetic acid. In this experiment, para-Chlorophenoxyacetic acid was synthesized from 4-chlorophenolate and chloroacetic acid using an SN2 reaction. The product obtained was determined to be the para isomer of Chlorophenoxyacetic acid. This was confirmed by the melting point of 157.3-157.9 ◦C. The percent yield determined at the end of the experiment was 37.83 %. The TLC analysis showed that P-Chlorophenol was less polar than P-Chlorophenoxyacetic Acid because it had an Rf value of 0.38 in comparison to the value of 0.33 on a 50:50 hexane and ethyl acetate solvent mixture. In the NMR comparison, it was shown that both the starting material of chloroacetic acid and product contained a peak of integration two around 4 ppm representing the acidic proton. In the FT-IR comparison, it was determined that the Chloroacetic acid and the para-Chlorophenoxyacetic acid both had an OH bond at 3416 cm-1 and 3429.72 cm-1 respectively. The Chloroacetic acid and para-Chlorophenoxyacetic acid also both had a carbon-oxygen double bond at 1648 cm-1 and 1654.81 cm-1 respectively. The para-Chlorophenoxyacetic acid also contained a peak at 1236.18 cm-1 which represents the C-O-C bond.

The oxidation number of an atom of any free element is ZERO. Means to say there is only one kind of atom present, no charge.

Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.

The purpose of the PGLO lab was to be able to perform a procedure known as a genetic transformation. We used a procedure to transform bacteria with the gene that codes for a Green Fluorescent Protein (GFP). The actual source of the GFP gene that we used in this complicated experiment is the bioluminescent jellyfish Aequorea victoria. This protein causes the jellyfish to glow under a UV light that was provided in the dark. After the transformation procedure, the bacteria showed their newly acquired gene from a jellyfish and produced the fluorescent protein, which as a result, causes it to glow. If the bacteria glowed in the dark, that was the initial sign that the experiment was successful.

Hypothesis: The purpose of this experiment was to investigate the question will different food sources affect the level of activity of detoxification enzymes in bean beetles? The class alternate hypothesis is different food sources will affect the level of activity of the detoxification enzymes in bean beetles. The null hypothesis is the different food sources will not have any effect on the level of activity of the detoxification enzymes in bean beetles. Experimental design: The independent variables in this experiment were the types of beans (bean 1 was mung beans and bean 2 was adzuki beans) and enzymes assays used.

2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.

The Uptake of Neutral red dye by saccharomyces cerevisiae in the presence of a metabolic inhibitor

Dispense .5 mL water into the already weighed conical vial, replace cap and face insert on its down side.

Science Essay: Red Cabbage PH Indicator Did you know that, instead having to buy expensive PH indicators, you can just use the juice from red cabbages? In this experiment, I will see how red cabbage can go from side dish to science. My hypothesis is that the color of red cabbage will change color based on a reaction with different substances to indicate whether it is an acid or base. According to the page madsci.org, red cabbage contains a pigment called ‘anthocyanin.’

The dye studied in this experiment comes from a group of dyes called azo dyes. These dyes are arranged as two aromatic rings connected by two double-bonded nitrogen atoms (R-N=N-R). The two nitrogen atoms being double bonded together cause the color that the dye produces. Azo violet is also used in labs as a pH indicator because of the fact that it turns from an yellow-orange color in acidic and neutral environments (and also when used as a dye) to a deep purple in basic environments.