Phila Melanogaster Egg Lab Report

2) Why are onion root tip and whitefish blastula areas that are ideal to view mitosis?

After gently blotting, weigh all three eggs separately inside their own individual weigh boats to the nearest 0.1g and record the weights in Table 1 at time 0. Place eggs 1, 2, 3 into separate culture dishes containing solutions of distilled water (0% sucrose), 14% sucrose, and 40% sucrose respectively. The sucrose must cover the entire egg inside the culture dish before proceeding to the next step. Place a napkin down on the lab counter, at 15-minute intervals, remove the eggs from the dishes with large spoon; place on napkin and wipe off all excess water each weigh boat must be clean as well before weighing; and again, weigh each egg separately. Record the weight in Table 1: Weight of Eggs vs. Time (minutes). Replace the egg in the dish for the next interval. After the experiment is complete, return the eggs to their container and each solution to its labeled container. Clean the dishes and weigh boats and return all materials to the setup

3. Identify the histological features (listed below) of the major endocrine glands on microscope slides.

To set up this experiment, two twenty-five gallon aquariums, 3 petri-dishes, 200 flies, rotten bananas, and yeast were used. The bananas chosen to be an accelerant for the growth of the yeast and were frozen so they would be easier to cut. The yeast was used because the drosophila melanogaster prefer this as a food source.  The vestigial and wild type flies were sexed (to determine their sex), sorted, and counted.  An initial population size of 100 total flies was decided so that it would be easier to determine the phenotypic percentage of the total population. Fly paper was placed in one of the sets of cages to impose a method of natural selection as well as the sexual selection which is being solely tested by the other set of cages.

animal in the early stages of growth when it’s basic structure and organ development is forming.

We did that for all three eggs and recorded that data down. Then we put the eggs into their cup marked with their solution and waited 24 hours to record the data again. We then got another egg for our control and measured it the same way as the other eggs and put in water for 24 hours. The reason we used this set of procedures is so our experiment would be very accurate, and easy to do so we would

The fruit flies were provided on March 27,2018. The fruit flies were incubated at 25°C for a couple of days. On April 3,2018 the F1 generation was examined which allowed to figure out the P generation by setting up crosses and collecting data. On April 10,2018 the identification, separation and counting of phenotypes in F2 generation was completed. On April 10, 2018 the fruit flies had to be counted correctly according to their phenotypes. Counting the sex of the fruit flies and analyzing for any specific mutation. On April 17,2018, F2 generation was counted and by that information that was obtained, a Chi-square test was made in order to prove or disapprove the hypothesis. Lastly, fruit flies were then removed from the cultured vials with larva and eggs and were placed in a sleeping chamber in order for the F1 generation to be counted and observed under the microscope. The reason why they had to be counted was to identify the sex and the phenotype from each individual fly. Then, the data was analyzed after the flies were counted, and a chi-square test was conducted. Since six vials were obtained from the instructor, two of the group members had to count 2 vials of F1 as well as F2 the other two members only had to count the fruit flies of one

The stain allows us to look at the cell morphology and observe it properly in the microscope. DNA is negatively charged because of phosphate so when the positively-charged methylene blue is added, then the charges attract and stains the DNA, allowing us to see the cell.

The cell grew for 24 hours at 37˚C. The next day the blue and white colonies were recorded. Then, picked 3 white colonies and 2 blue colonies. Grew the culture for another night at 37˚C with the shaker incubator set at 250

Significant changes in internal organs development can be revealed from larval and pupa stages in complete metamorphosis. According to [1] between day one to day thirteen, the chrysalis shows development of numerous aspects of morphology which include wing veins, midgut, flight

we said goodbye and placed them in the fly morgue. We allowed the F2 larval

This is the final step of the experiment. The shell-less egg was now placed into a new cup, but very carefully, as the egg was more fragile than when it was soaked in vinegar. The egg in the new cup was then covered with water. The egg sat in the water for a 24 hour time period once again. During this 24 hour time period the eggs appearance and size were once again recorded. After the time period had completed, the egg was removed from the water and was very carefully patted dry. Since the egg was now swollen, extra care was needed when holding the egg. The swollen egg was placed on the scale on last time and its mass was recorded as M3.

The purpose of this lab is to observe how the decalcified egg reacts to different solutes. This lab is to show how osmosis and diffusion works. Osmosis is the movement of water within a cell. Diffusion is the movement of substances (solutes) within a cell. I think that when examining the egg’s cells, the plasma membrane, nucleus, and nucleolus will be visible. I think that once the egg is placed in sugar, the egg will shrink. The reason for this is that sugar will reduce the amount of water within the cell. Less water means that hypertonic will take place, causing the cell to shrink. I think that once the egg is placed in water, the egg will expand. The reason for this is that the water will increase the amount of water within the cell. More water means that hypotonic will take place, causing the cell to expand.

In conclusion, the data that was collected helped tell us that the solutions greatly affected the cell of the egg because it caused the egg’s mass to increase than decrease. Also saw how the solutions played a role in the phospholipid bilayer and saw how it altered the shape of the egg. However, it does not support my hypothesis on the affect the egg would undergo onced placed in sugar and water but sugar was not used in this experiment, but water was used and it didn’t affect the shape of the egg, all it did was cause the egg to be in a isotonic state. According to Khan Academy (2017), states that a cell is isotonic “When the extracellular fluid has the same osmolarity as the cell, there will be no net movement in or out of the cell”. Which

as single cells, with 2- to 4-cell clusters occasionally observed (Fig. 1A, arrowhead). On the second day, the cells formed small solid spheroid-like cell masses (Fig. 1B). From the fourth to eighth day, the size of the solid spheroid-like cell masses gradually increased (Fig. 1C-E). From day 14 on, hollow lumens began to appear in the spheroid-like structures (Fig. 1F, arrow). With time increased, the number of spheroid-like structures with one or more lumens lined by one or more layers of cells also increased (Fig. 1G-J). The formed secretory coil-like structures and coiled duct-like structures [12] were similar to the secretory coils and coiled ducts of native human eccrine sweat glands, respectively (Fig. 1K).