Photosintersis Experiment

Light intensity is a key component in photosynthesis, amongst carbon dioxide and water to sustain a suitable rate of photosynthesis. Chlorophyll absorbs the light, causing photoexcitation and the formation of NADPH and ATP with production of O2 as a by-product. The Calvin Cycle takes the NADPH and ATP to reduce CO2 into sugars (CH2O), and return NADP+ and ADP + Pi to the light reactions. The process will then repeat. (Reece, et al, 2015)

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

Test tube six contained an elodea and was placed in the dark. It was hypothesized that, after a day, the test tube would be yellow in color. Upon observing, the hypothesis was proven correct because test tube six had remained yellow. This is because the elodea was placed in the dark where it could not receive light to photosynthesize and remove carbon dioxide from the

The purpose of this experiment is to determine the maximum absorbance of fast green, and the chlorophylls, also in the case of fast green create a concentration curve to determine an unknown substance. Each test will use the spectrophotometer.

The null hypothesis will be that the test tubes with an increase in temperature, pH values, enzyme concentrations, and substrate concentration will have a very small color change or no color change at all. The alternate hypothesis is that the test tubes containing an increase in temperature, pH values, enzyme concentrations, and substrate concentration will all have an intense color change; the more the change, the more intense the color change will be.

The procedures for experiment A, B, and C all start the same. The first step is to put on goggles and get the data collection device set properly. The labquest needs to be plugged into the colorimeter accurately so that a click is heard when putting it in. The labquest needs to be reading digitally and the colorimeter needs to be set to 635 nm. Then shake the chloroplast solution and take a clean cuvette and fill it with 3 mL of distilled water, 3 drops of the chloroplast solution, and cap it. This is used as a blank to calibrate your labquest. Double check that the labquest is reading absorbance, this assures that the colorimeter is plugged into the labquest accurately. Insert the blank into the colorimeter and hit the calibration button. Take out the blank and empty it. The labquest is now set to experiment with. Make sure that the heat bank is set in front of the lamp and that the lamp is on. The cuvette must be placed on the opposite side of the heat bank in the path of light in the box so that no other light can interfere with the experiment.

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

12. Describe two ways (two different things you could measure) to estimate the rate of photosynthesis in a particular plant. What specific measurements would you need to make for each in order to estimate GROSS photosynthesis?

When using algae beads and a CO2 indicator, the process of Photosynthesis and Cellular Respiration can be measured. In this experiment the intensity of light will be altered in each trail, and the rate of Photosynthesis will then be measured. As you rise from low light intensity to higher light intensity, the rate of photosynthesis will increase because there is more light available to drive the reactions of photosynthesis. However, once the light intensity gets high enough, the rate won’t increase anymore since there will be more-light than water and CO2; there will not be enough components from light, water, and CO2 to create the process of Photosynthesis. As CO2 dissolves and the amount of CO2 goes up, the pH will lower, which means the solutions color will change varying form red, orange and yellow, all pending on what the pH is at. CO2 will be produced from respiration, all while photosynthesis absorbs the CO2. This means that when the rate of photosynthesis is less than respiration, pH levels will decrease, and CO2 concentration will increase. Vis versa, when pH levels increase

The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the

4. We observed the test tubes and compared the colors produced from the reaction to the color palette in 1 minute intervals for 5 minutes total.

The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control groups (Control 1 or 2) showed any signs of photosynthesis. Control 1 was exposed to light, but contained no photosynthetic organelles thus the absorbance throughout the 30 minutes varied minimally, mostly staying stagnant. Control two which contained chloroplast but was not exposed to any light failed to lower the absorbance at all and in fact increased the absorbance over the 30 minutes. However, the treatment group contained both and ultimately performed photosynthesis as we expect therefore, confirming our assumption that chloroplast were the organelles required for photosynthesis in plants and that light is required to perform said photosynthesis. The treatment group, containing both the chloroplast and being exposed to light provided evidence that photosynthesis was taking place as the absorbance lowered at each 10-minute interval. Having a less absorbance would be desired because as DCIP became reduced we would expect the solution to become more and more clear, thus less

Photosynthesis occurs in the palisade mesophyll of plant leaves. During photosynthesis, carbon dioxide is broken up into 6 carbon for the sugar molecule and 12 oxygen. In this lab, I determined the rate of photosynthesis in spinach

However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.

In this experiment I will investigate the affect in which the light intensity will have on a plants photosynthesis process. This will be done by measuring the bubbles of oxygen and having a bulb for the light intensity variable.