The purpose of this lab is to determine which pigments in a plant support or effect photosynthesis, based on starch production, which wavelengths of light are involved in photosynthesis, and identify plant pigments found in a plant leaf by means of paper chromatography.
Photosynthesis is a biochemical process in which plant, algae, and some bacteria harness the energy of light to produce food. Nearly all living things depend on energy produced from photosynthesis for their nourishment, making it vital to life on Earth. It is also responsible for producing the oxygen that makes up a large portion of the Earth¡¦s atmosphere. Factors that affect photosynthesis are light intensity and wave length, carbon dioxide concentration, and temperature.
However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.
To understand pigments and their part in the essential process of photosynthesis, we constructed an experiment to discover first-hand the effectiveness of specific pigments found in pimento leaves. These two exercises would specifically focus on the difference in polarities and the different wavelengths at which each pigment absorbs light. The ultimate source of energy for most organisms is sunlight. This research expresses the importance in understanding the driving force behind crucial photosynthetic organisms that are responsible for converting solar energy into chemical energy and ultimately the fixation of carbon dioxide. The polarity of three of the specific pigments studied, chlorophyll a, chlorophyll b, and xanthophylls, was determined by separating the plant pigments by paper chromatography and calculating their Rf values. Once the pigments separated along the paper chromatography strip, we cut the different pigments bands and eluted them from the paper into a beaker filled with acetone. We were then able to analyze the wavelengths of light absorbed by the pigments through the use of a spectrophotometer. We predicted the wavelengths for chlorophyll a, chlorophyll b, anthocyanidins, xanthophylls, and carotenoids. From the first experiment we were able to determine that xanthophylls, which traveled the farthest of the three, was
Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special way of using the ATP they produce. Photosynthesis is a process where plants harness the sunlight they receive and they produce carbohydrates, as well as oxygen for living things and other plants. Now the sunlight ultimately powers the process of
Autotrophs make their own food, by using the energy of the sun to make sugars from carbon dioxide and water. Heterotrophs depend on photosynthesis to consume carbon dioxide and then produce oxygen (respiration). Oxygen and an energy source create ATP, which powers cells. All life ultimately depends on photosynthesis, whether directly or indirectly. . Light is the most important component that affects photosynthesis, because it provides the energy necessary for driving the reaction. Without light, plants cannot create ATP or NADPH, which is how the plant creates sugars to feed itself, therefore causing it to die. In the oxidation and reduction process, sunlight is the energy source that moves electrons from oxygen to carbon.
Photosynthesis is the procedure in which green plants use sunlight, carbon dioxide and water to make food and oxygen and cellular respiration is the process where cells use this food to
Without photosynthesis we would not be able to receive energy. We should be more appreciate of plants, without them we would not survive. This paper will explain the basic components require for photosynthesis, the role of chlorophyll, how energy is transferred, and photosystems I and II and the most precious product results of photosynthesis.
For the purpose of the experiment , photosynthesis would require water , carbon dioxide and light. As the light produce a certain wavelength, it would show a light color for human eyes to appear. In the experiment we have tested four types of color which would have start the process of photosynthesis. It would mean that if there is light that would create a type of wavelength which would start the process of photosynthesis by starting to take carbon dioxide with water and to produce glucose and oxygen. As a way to test for those types of light we would take the spinach and place it inside a chamber , then we would take the carbon dioxide sensor and place it inside the chamber with a the type of light traveling through the chamber. The carbon
One of the most important bological processes on earth is photosynthesis. Plants that comprise the majority of the food we consume need photosynthesis in order to produce life sustaining energy. This energy is harnessed by the sun. If it were not for photosynthesis to capture and use this vital energy, life itself would be minimal or almost non-existent.
The effects of light intensity and light wavelength on photosynthesis was observed in two different experiments and closer study of different pigments in spinach was observed. For the experiment where the effects of light was observed, it was found that a light intensity of 30 cm, wavelengths of blue and red lights show the greatest photosynthetic activity. The different pigments present is spinach were also observed and it was noted that though chlorophyll is the most abundant in plants, there are other pigments present as well.
“Photosynthesis is a biochemical process for building carbohydrates using energy from sunlight and carbon dioxide taken from the air”, (Morris, J. (2016) Biology How Life Works. New York, NY.). It is a system that uses plants and specific algae to synthesize molecules from both water and carbon dioxide. The oxygen we breathe and the food we eat is fueled by photosynthesis because it is an energy source. Photosynthesis occurs in eukaryotic and prokaryotic organisms and some examples are humans, trees, and plants. Biological systems use photosynthesis as an energy source. Where there is a source of sunlight, there is a chance
Some pigments move further than others because of the size of their particles. Smaller, lighter particles will move through the filter paper more swiftly than larger, heavier particles. This is true because smaller, lighter particles are more soluble in solvent, thus allowing them to move further. In addition, a pigments affinity for paper will affect its distance travelled. The more polar a substance is, the greater attractiveness it will have to paper through hydrogen bonding (Jeffrey & Saenger 149). Therefore, the pigments particle size, solubility, and attractiveness to paper will determine the distance it moves.
We need photosynthesis because photosynthesis makes air for us to breath. Photosynthesis makes food by taking in our Carbon Dioxide, water, and sunlight to make our sweet glucose/sugar that we eat every day. Photosynthesis cycles matter in and out of organisms by making glucose that we and other organisms eat then we go to the bathroom and the fluids mix with the groundwater and then it happens all over again. Photosynthesis cycles energy thru ecosystems by taking in carbon dioxide and making it into oxygen that we breath. I know this because in the article Modeling photosynthesis it says that Plants are producers. This means that they can produce food using light energy instead of having to eat, or get nutrients in other ways.