Photosynthesis Lab

Measuring the Rate Oxygen Production using an Oxygen Electrode Chamber in Photosynthesis of Spinacia oleracea with Varying Light Intensities

The rate of photosynthesis can be determined different ways. Because oxygen is a product of photosynthesis and the Elodea plant is submerged in water, the oxygen is released in bubbles that rise to the surface of the water in the beaker. In this experiment, the rate of photosynthesis for each degree of light intensity can be measured by counting the number of bubbles released every 30 seconds for five minutes at each distance. The rate is the number of bubbles released per minute.

-Measuring the pH of a solution (such as in the lab we had) could also help determine the rate of photosynthesis. You would need a much more specific pH meter, but generally, if pH goes down, the level of CO2 is higher, meaning more cellular respiration. Higher pH means there’s less CO2, so more photosynthesis.

When using algae beads and a CO2 indicator, the process of Photosynthesis and Cellular Respiration can be measured. In this experiment the intensity of light will be altered in each trail, and the rate of Photosynthesis will then be measured. As you rise from low light intensity to higher light intensity, the rate of photosynthesis will increase because there is more light available to drive the reactions of photosynthesis. However, once the light intensity gets high enough, the rate won’t increase anymore since there will be more-light than water and CO2; there will not be enough components from light, water, and CO2 to create the process of Photosynthesis. As CO2 dissolves and the amount of CO2 goes up, the pH will lower, which means the solutions color will change varying form red, orange and yellow, all pending on what the pH is at. CO2 will be produced from respiration, all while photosynthesis absorbs the CO2. This means that when the rate of photosynthesis is less than respiration, pH levels will decrease, and CO2 concentration will increase. Vis versa, when pH levels increase

This lab was conducted to explore the light energy, pigments and the rate of photosynthesis in magnolia leaves. In experiment one a magnolia leaf was used to see the separation of primary and accessory plant pigments using a process called paper chromatography. The importance of this process was to discover which pigment had the highest band along a piece of filter paper and identify various plant pigments in a magnolia leaf such as xanthophyll, chlorophyll a, chlorophyll b, and carotenoids that aid magnolia leaves during photosynthesis. Based on the conducted experiment, it can be concluded that chlorophyll a was the pigment that showed the highest band on the piece filter paper which means that chlorophyll a is the primary pigment in photosynthesis

At the end of each minute, record the number of floating disks. Then swirl the disks to dislodge any that stuck against the side of the beakers. Continue until all of the disks are floating. Create a graph of floating chads vs. time and analyze whether the green or reddish-purple leaves photosynthesized at a faster rate.  Results

Photosynthesis is essential to all living organism such as animals and plants. Photosynthesis is a process used by plants and other autotrophs to capture light energy and use it to power chemical reaction that converts carbon dioxide and water into oxygen, carbohydrates and water. (Textbook: Principles of Biology). The reactants and the products of photosynthesis are:

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

As the table lamp is positioned nearest to the pond weed, it shows highest rate since higher light intensity means more photons are striking at the surface of leaves. When the light intensity rose from low to high, it will cause the rate of photosynthesis to increase due to more light energy presents to operate the reactions of photosynthesis. Generally, when the intensity of light is high, a greater rate of photosynthesis will be achieved. However, this rate has a limit, and once that limit is reached the rate cannot be increased to past that limit. It is because the oxygen gas will start to compete for RuBP carboxylase with carbon dioxide at a certain light intensity, whereas the more oxygen gas binds to the enzyme, the less glucose is produced,

For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.

The disc floated back from the top because of oxygen being produced by chloroplast in the leaves. The disc in the dark floated because they still had a little bit of oxygen with them but because the leaf were in the dark the oxygen did not fill because of the chloroplast was in the dark. The chloroplast in the dark probably came out because .The test was to see if the leaf disc

This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.

Photosynthesis is a vital process that autotrophs use to transfer light energy into chemical energy. Photosynthesis ultimately produces O2 and glucose. It, like many other biological processes, can be affected by environmental variables. The variable that we altered in the following experiment are intensity, light wavelengths, and pigment types. In order to do this, we conducted three experiments. In the first experiment, we examined the effect of light intensity by placing vials with chloroplasts with DPIP at different light distances in which the results varied. Initially, 30cm away was the most effective for photosynthesis. Then 24cm appeared to be the most effective. Followed by 49cm at minutes 25 and 30. In the second experiment, we

Based on the findings by Robert Hill it is hypothesised that in the use of DCPIP as a coloured indicator for the conductance of photosynthetic reactivity of the chloroplasts will allow a continuance of the production of oxygen and electron flow, even in the absence of carbon dioxide, and that

A plant is any of the boundless number of living beings within the biological kingdom Plantae, these species are considered of low motility since this species generally generate their own food by sunlight. They incorporate a large group of commonplace life forms including trees, forbs, bushes, grasses, vines, plants, and greeneries. In this task we are experimenting the relationship between light and plant growth by growing plants in three different lights which are red light, blue light and white light. As I stated above that plants generate their own food by sunlight. Sunlight can be broken up by a prism into respective colors of red, blue, orange, yellow, green, indigo, violet and white. All this lights have specific