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Photosynthesis Lab Report

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All of the T-streak showed growth however, not all of the bacteria formed colonies. Pseudomonas aeruginosa had no colonies therefore, the colony morphology were not applicable. The error could have been to not spreading the bacteria enough using the T-streak method. P.aeruginosa, Enterobacter aerogenes, Escherichia coli and, Klebsiella pneumoniae all tested as gram negative. The lab manual confirmed the experiments findings to be correct and each one were pink colored on the slide under a microscope. If the result had varied from gram negative a reason could include leaving the decolorizer on the slide for one minute instead of ten seconds. Doing this could make the slide appear any color other than pink. The results could also have varied …show more content…

P.aeruginosa, E. coli, and K. pneumoniae were all non-motile and the lab manual confirmed it to be incorrect. E. aerogenes results were motile which were confirmed by the lab manual. P.aeruginosa and E. coli should have been motile. The results could have been incorrect due to contamination of bacteria or not enough bacteria were used to get an adequate result. This could result from the bacteria not be spread throughout the medium causing the sample to look non-motile. The oxygen requirement test showed all four of the bacteria to have facultative anaerobic results. The lab manual confirmed all the bacterium except P.aeruginosa. P.aeruginosa should have obligated aerobic results. The results varied due to contamination of the bacteria used to cause a misconception. P.aeruginosa had an obligate aerobic result because it lives with the presence of oxygen and dies without it. The results could have varied due to contamination of the bacterium.
The color of the EMB matched the lab manual except for the manual did not specify E. coli to have purple and green colors. The results could have varied if enough bacteria had not been scraped on the petri plate to show the

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