Photosynthesis Lab Report

The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test

The purpose of this experiment was to find out the nutrient content of unidentified food samples and to use chemical reagents to see if the unidentified foods for sugar, starch, protein and fat (lipid) content. After comparing the color changes in each unknown substance then you will be able to see the amount of sugar, starch, protein or fat there is in the unknown substance.

of 116 bpm, respiratory rate of 18 breaths/min, and blood pressure of 86/54 mm Hg. The

7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.

In addition a small rise in breathing rate and this is called anticipatory rise, this happens when exercising. The average reading for breaths per minute during exercise is 23-30. This shows that with more blood pumping through the body more oxygen is needed to keep the body at a sustainable rate to help our body create more energy. Our breathing rate will keep increasing until

After the exercise, we then took the measurements of our pulse, breathing rate and temperature too to see the change. Once we had completed this the first time, we then did it 2 other times, so in total 3 time so that the data was reliable and trustworthy. (Stretch, B., & Whitehouse, M. (2007).

7. After obtaining 10-20 seconds of stable heart rate values, rise rapidly to a standing position. Continue to record until the baseline heart rate has been achieved or until the end of the run. Data will be collected for 400 seconds.

The heart rates of participants was tested before the step test, one minute, two minutes, and three minutes after the step test was performed in this experiment. Since heart rate increases while someone is performing physical activity, it was expected that heart rates of the students would be higher than before the step

3.Measure and add 0.5g, 1.0g, and 1.5g of sucrose into 3 of the test tubes. Do not add sucrose into the 4th test tube because this will be the control. Lightly shake the test tube to mix the contents together.

test the pH of the amylase a drop of the solution should be put on pH

For this experiment, we have to prepare our phosphorylase which extracted from a potato. We prepared by weighed about 250 grams of peeled potato and cut it into cubes. The extracts then blended with 100mL of 0.1M NaF. After filtered the contents into a clean 250mL centrifuge bottle, we centrifuged it for 3 minutes. Then, separated the supernatant into a centrifuge bottle, which is our phosphorylase preparation. The enzyme assay used in this experiment today is the iodine test. As the iodine reacts with starch, it will form a brown, blue or black precipitate due to the iodine ions forcing into a linear arrangement. The endpoint of the enzyme reaction indicates the presence of starch by using the iodine test to determine. The faster the endpoint is reached, the less active the phosphorylase is.

The results of this test show that it provides a valid test to estimate aerobic capacity and shows there is a small measurement of error. A polar heart rate monitor was used to measure the heart rate with a step of 30cm in height with a metronome with a beat at 15 steps, per minute and increased by 5 steps every minute for 5 stages or until 80% of the maximum estimated heart rate was reached. The results demonstrated that the Chester Step technique is a valid predictor of aerobic capacity in males and females from a wide range of ages and fitness levels.

Add the same amount of Benedict’s solution in each tube and for the ones that need iodine use the same amount.

Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates, proteins, lipids, and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound.

During the Benedict's test, the contents of tube B did not change, indicating the absence of sugar in that particular substance. However, the contents of tube A did change orange indicating the presence of sugar in that substance. During the Lugol's test, the content of tube A did not change dark purple indicating the absence of starch in that substance However, the content of tube B changed to dark purple