Photosynthesis Lab Report

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

The green pigment involved in photosynthesis is chlorophyll. Chlorophyll is green in appearance because it absorbs red and blue light, making these colours unable to be seen. It is the reflection of the green light that reaches out eyes, giving chlorophyll a green colour. This green light that can be seen cannot be used by the plant for photosynthesis. Therefore, theoretically growth should be inhibited in the plants only exposed to green light.

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the

The process of photosynthesis, by which light energy is used to convert inorganic compounds into organic substances with the release of oxygen, may be the most important biological event sustaining life (Keir et al. 2017). In the light-dependent reactions, the chloroplasts of a plant use the pigment chlorophyll to convert light energy into chemical energy. This energy is used to split water and produce oxygen (Eller et al. 2015). The energy is later used in the light independent reactions, where carbon dioxide (CO2) undergoes carbon fixation with the aid of enzyme rubisco, because it catalyses both carboxylation and oxygenation reactions and most of responses of photosynthesis to light, CO2, and temperature (John Evans 2013).

Question Do green or reddish-purple lettuce leaves photosynthesize at a higher rate under red light? Hypothesis Green lettuce leaves will photosynthesize at a faster rate than reddish-purple lettuce leaves when exposed to red light because reddish-purple lettuce leaves reflect red light and will thus have fewer photons to excite light receptors and begin photosynthesis. Materials: Baking soda Diluted soap solution Plastic syringes without needle Green lettuce leaves Reddish-purple leaves Hole punch 4 small beakers

Page 1 : http://mw2.concord.org/public/part2/photosynthesis/page1.cml 1. What color light has the highest frequency? (a) Red. (b) Yellow. (c) Green.

This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.

To test the rate of photosynthesis in spinach leaves, we placed a spinach thylakoids mixed with dichlorophenol-indophenol (DPIP) in front of various lights treatments. In total, there were 5 cuvettes which all received different lighting. One was a control which contained no thylakoids, another had no light at all, the third cuvette was completely open to all possible light, the fourth was covered with a green translucent paper, and the final cuvette was covered with a red translucent paper. In five minute intervals, the transmittance was measured using a spectrophotometer which allowed the rate of photosynthesis to be measured. Upon completion of this lab, the white light produced the highest rate of transmittance shortly followed by the red light and then the green light.

The hypothesis for this part was that shorter wavelength of light will have a greater increase the consumption of CO_2 by the spinach leaves than the longer wavelengths. Four different colors of light were tested: purple, green, red, and yellow. The same steps as the first part were repeated for this part, but the only difference was using different colored lights instead of white light. The dependent variable for this experiment was the CO_2 concentration in the Testing Chamber, while the independent variable was light.

The final conclusions of this experiment are that the closer the light source is to a plant, the more light it can absorb, colors farthest from green on the visible light spectrum are most likely to absorb the most light rather than colors closest to green, and only regions containing chlorophyll on a plant can absorb

(Photosynthesis. (2008, August 2). Retrieved December 5, 2014, from http://biology.clc.uc.edu/Courses/Bio104/photosyn.htm). The chlorophyll is built into the membranes of the thylakoids. Because chlorophyll absorbs blue and red light waves it appears green. The green light waves that aren’t absorbed give us the ability to see it.

For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.

However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.

The Effect of Light Intensity and Temperature on the Rate of Photosynthesis Aim The aim of my experiment is to determine whether intensity of light and temperature would affect the rate of photosynthesis in a plant. To do this, I will place a piece of pondweed in varying light intensities and temperatures, and observe the amount of oxygen being given off. I am using pondweed because of its unusual quality of giving off bubbles of gas from a cut end, when placed in water. Introduction Photosynthesis occurs only in the presence of light, and takes place in the chloroplasts of green plant cells.