Planarian Segmentation and Regeneration Summary We started out by picking our worms out of their original containers then transferring them to a petri dish, then pouring in enough spring water via a pipette to sustain the worm, without providing too much as it could potentially escape. The water was necessary otherwise, the worm would dry up and die. We then took a small razor blade and made our initial incisions, having the option to cut once or cut up to three times. I chose to cut my planarian only once, without the assistance of a microscope and I used a small ruler before making my initial cut and then shortly after. I had originally predicted my worm’s head portion would grow to be bigger overall between the two and grow faster than
6. Continue cutting the skin of the earthworm towards the anterior end. It is best to use an upward-pointing direction with the scissors. As you cut the skin, pin back the skin to expose the gut.
This same procedure is repeated to transfer worms from the dpy-13 starter plate to the OP50 seeded plate labeled “dpy-13”. These plates are then both incubated upside down at 20˚C for 48 hours. This incubation gives the worms time to grow in their new environment.
This article studies the ability of regeneration possessed by the planaria. Morgan (1898) observes in different methods how the planaria are cut into smaller pieces, and then he observes the pieces to see if the planaria will regenerate. When the planaria are cut near the head, the pieces do not appear to regenerate, and the pieces cut too small also do not possess the ability to regenerate (Morgan, 1898). Pieces cut from the middle and the end piece of the planaria, however, have shown results of respawning from within the old tissue. When the author tried to fuse two sampled pieces together, he observed that the pieces formed a union (Morgan, 1898). However, the fusion did not last long, and the pieces died long after fusing together (Morgan, 1898). It is likely that the samples were not able to complete the regeneration after the union emerged. I will be able to use this report for my lab report as a reference to the process of taking samples from a live planaria, sampled from the likely
* 3 to 5 times more inclined to get growth of the mouth, neck and3 to 10 times
What you will need for the dissections of the crayfish is: a crayfish dissections kit, a crayfish, a place for you to put the crayfish, gloves, and goggles. When starting to dissect a crayfish you will want to use scissors at the beginning and start cutting from the side of the abdomen up to its eye on both sides. Once you have done that you will want to lift the carapace and use a probe-like stick to get the muscles and maybe the heart off of the inside of the carapace. After you have
Before starting the observations, the planarians had to be cut into sections using a scalpel, in this case, they were cut into three. To make them easier to cut, the were chilled on ice to slow them down.The flatworms were then put into separate labelled petri dishes, where they were then placed in a cool and dark area. After a few days, the worms grew and were easier to observe. From this lab, it can be concluded that planarians will regenerate no matter how they were cut. Wormy and Wormy 2.0, were both trisected transversely and were thought to grow into six new planarians, three coming from each worm, and one of them possessing two heads. The results observed for Wormy fully supported the hypothesis, and the three new planarians had a head,
Seasonal shifts to freezing temperatures creates harsh conditions for animals to endure. There are two main strategies for surviving the winter when temperatures fall below 0°C: i) freeze avoidance, where the animal prevents body fluids from freezing by migrating, escaping to thermally buffered environments, or supercooling of body fluids (Lowe et al., 1971; Storey & Storey, 1988; Burke et al., 2002), and ii) freeze tolerance, in which freezing of body fluid is allowed, though ice crystal location and size is controlled (Storey & Storey 1984; 1988; Zachariassen & Kristiansen, 2000; Duman, 2001). The most well studied freeze-tolerant vertebrate is the wood frog, Rana sylvatica (Storey & Storey, 1984; Layne & Lee, 1987; Lee et al., 1992; Churchill
Our efficiency in preparation would soon reflect into our audience’s response, especially with the delightfully booming amount of responses received the first day we opened our registration to the public. On April 24, we used Noah as a test registration before opening the registration to the public the following day, this still meant that there was one less person in the market. Then, on April 25, we opened registration to the public and informed them of it through an advisory announcement for the sophomore and junior classes and a morning email to all. Since our group was one of the first to start advertising, we had little to no competition for our target market at first. As we had expected, a large number of 48 registrations came in due
There once was a scientist named Gary Gee Gager. He was the best in his department. He studied and changed the way animals evolve and grow and how to change them. In his lab he had two test bunnies name Greg and Bella. Gary wanted to see if he could double their growth and size. But Gary needed perhe ever thought of. Over the course of eight years in jail this is all he thought about. First he regained all his research and found a shocking discovery why they didn't age. He apparently made them their bodies heal to times faster not allowing them to age there body never got weaker! Over the course of time he studied the two bunnies. It seemed the more time they grew so did their healing abilities. 4 years passed the bunnies still grew. They were
can be seen that all the developmental phases are seen in an overlap or either skewed more
For many women, breast size, shape and proportion play an important role in self-perception and confidence. Perhaps that’s the reason why breast augmentations are amongst the most requested cosmetic surgical procedures. In fact, according to the top breast augmentation surgeons, breast implants are an appropriate treatment for a number of breast concerns including asymmetry, reconstruction procedures following breast cancer surgery, and breast deflation following pregnancy and significant weight loss.
The cost of breast augmentation can vary depending on whether you have saline or silicone implants. There are some important questions to ask, as you should not base your decision about where to have surgery only on price.
The accurate segmentation of the bone from the magnetic resonance (MR) images of the knee is a very important research in medical image segmentation. Recently various segmentation algorithms were proposed for knee segmentation. These algorithms are time consuming and semi-automatic, due to the appearance and shape of the bone. To overcome above mentioned problems we propose automatic and accurate segmentation's to automatically segment the non pathological knees. The segmentation is performed using five techniques such as affine registration, non-rigid registration, tissue classification, bone segmentation and BML
Acorn worms get their names Hemichordates from having some of the characteristics of a chordate. “Hemi” meaning half and then adding chordate. They are both also part of the deuterostomes family by having their first opening to be the anus. There are certain characteristics that make acorn worms part of the deuterostomes family including a bilaterally symmetrical body, radial cleavage, enterocoelous coelom, and invertebrate. Bilaterally symmetrical meaning that the acorn worm can be open right down the midline and both sides of the worm would be exactly the same. The enterocoelous meaning that the acorn worm has a true body cavity which forms of the embryonic mesoderm as the evaginations of the gut and also that the anus is the first opening
As with any other radiographic method, optimum interpretable diagnostic images can only be achieved with careful quality assurance in patient positioning, in selecting appropriate exposure parameters and during processing.