Plant Viruses : A Large, Unique Family Of Plants Viruses

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Geminiviruses are a large, unique family of plant viruses that have the ability to infect multiple different plants around the world. [1, 2] Geminiviruses consist of four genera, Mastrevirus, Curtovirus, Begomovirus, and Topocuvirus based on host ranges, vector specificities, and genome organizations. Characteristics of geminiviruses include their circular, single-stranded DNA genome and geminate-shaped virus particles. These viruses-- exhibit both prokaryotic and eukaryotic features-- replicate in the nuclei of the host cell and depend on host machineries for transcription [1]. The majority of the Old World begomoviruses have monopartite genomes while the New World begomoviruses are exclusively bipartite. [3] Whiteflies, often found on the underside of leaves are typically responsible for transmitting the monopartite begomovirus Ageratum yellow vein virus (AYVV) which has been previously characterized in Escherichia coli (E. coli) cells harboring single copies of the AYVV genome [1, 4]. The replication-associated protein (Rep) of geminiviruses has displayed similarities with those of prokaryotic plasmids [1]. Additionally, geminiviruses utilize rolling-circle replication as the primary mechanism for replication which is analogous to prokaryotic mechanisms found in some eubacterial plasmids and bacteriophages. [1] The research presented in this study focuses on the identification of a novel prokaryotic promoter called the AV3 promoter in the genome of AYVV-NT that may

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