Pneumocystis Jirovecii Research Paper

The next step of the project included preparing a Gram stain to discover the cell shape, arrangement, and if the bacteria is gram positive or

Table 3 shows Gram stain results that indicated C. Freundii as a gram negative bacterium in rod shapes scattered in singles and some in pairs. Each gram stain produced the same results. The Bartholomew and Mittwer method of endospore staining indicated that C. Freundii tested negative for endospore formation. Table 4 shows the biochemical test results of the unknown and the official test results for comparison.

Two smears of the unknown bacterium #5 were inoculated while the second smear was used for a back up. The unknown bacterium dried for at least forty minutes. After the smears dried, the slides were heat fixed two times to ensure the stability of the organism. The slide was placed on top of the staining rack then over the small sink.

This experiment can be enhanced in many ways. The amount of alcohol used as treatments could be lessened to measure the viability of A. salina more accurately. The amount of cysts in each Petri dish was not consistent because there

Figure #1 This picture show the gram stain of unknown #3. The image is under 1000x magnification using oil immersion technique. Gram (-)

pneumoniae were EMB, Oxidase, Citrate, Gelatin, Glucose, Lactose, SIM, Methyl Red, vogues Proskauer and phenylalanine. The start of the Biochemical test consisted of inoculation on regular nutrient agar and incubating for 48 hours. After incubation, gram staining was done to identify the bacterium as either gram positive or negative along with the shape of the bacterium using a microscope. The bacterium was then inoculated on EMB based on the identification of it being gram negative. After the inoculation on EMB, Oxidase, Citrate, Gelatin, Glucose, Lactose, SIM, Phenylalanine, and MR-VP were inoculated and incubated for 5 days. The results of each biochemical were observed and

Spores can be stained using specific dyes, such as malachite green, that are absorbed by spores in the presence of heat (Noonan).

Klebsiella pneumoniae is a gram negative bacterium so gram staining of the sputum would be a good place to eliminate all the gram positive bacterium.

It was a snowy day in December. When my mom organized family albums, I noticed a picture of my great grandfather on my mom's side. My mom told me that my great grandfather was an arch electric welder and he passed away at his early 60's in China. I wondered why he died so early. My mom said, "He suffered from pneumoconiosis. It's a type of disease where you have too much dust on your lungs, and it turns your lungs to black and makes them harder than stones." After I got the answer of my great grandfather's death, I wanted to investigate pneumoconiosis facts in the United States and in the world.

A great deal of cultural tests and physiological procedures were performed on the unknown before the conclusion of P. aeruginosa was made. The first test preformed was Gram’s staining which involves the use of crystal violet, Gram’s Iodine, Ethyl Alcohol, and Safranin. Gram staining is known as a differentiating stain, since it can determine the presence or absence of peptidoglycan in the cell wall. The unknown bacteria were Gram stain negative. After that the motility of the cell was tested using a hanging drop slide. The slide was examined, but the results were inconclusive due to the inability to make a clear distinction between motility and Brownian motion. This test was not

Oswald and a group of his colleagues helped discovered through various experiments that Pneumococcus bacteria causes pneumonia. Pneumococcus can grow and live on the body, however, it could also grow in solids or liquids. In the year of 1928, Fred Griffith made a publication on the bacteria Pneumococcus, with this it was discovered to stains S and R in which S was smooth and R was rough, the reason for this was because the S had a layer of sugar unlike the R. It also turned out to be when the mice was injected with the S it would die, and when injected with the R it would live (was not infested with pneumonia). He also predicted that a variety of strains could derived from one sources, so he did more experiments on the mice. Griffith heated

Streptococcus pneumoniae is a gram-positive bacteria that is a facultative anaerobe. This strain of bacteria can cause five different bacterial diseases. Streptococcus pneumoniae can cause pneumonia (an infection of the lungs), otitis media (an infection of the middle ear), sinus infections, bacteremia (an infection of the bloodstream), and meningitis (an infection of the covering around the brain and spinal cord). The most common disease it causes is pneumonia called pneumococcal pneumonia (Travelers' Health). Pneumonia is an infection of the lungs that causes the lungs to fill with fluid. This makes it difficult to breathe causing less oxygen to go to the bloodstream making this

Streptococcus pneumoniae is found worldwide. The common host is the human body, in which it often does not cause disease but at other times it can cause diseses in particular, pneumonia. It also causes otitis media, bacteremia, meningitis, peritonitis, and sinusitis. The route by which this organism is spread is from human to human in the form of aerosol droplets. When inside the host the organism’s primary site of pneumococcal colonization is the nasopharynx. From this site it can aspire to the lungs, eventually spread to the blood and traverse the blood-brain barrier to the meninges, once inside the blood it can cause infections throughout the body. Symptoms of the disease include sudden

For this method, the plates containing nutrient agar was divided into 4 parts. In each part, a bacterial isolate was spotted in duplicates. The plate was incubated at 30◦C for 24 hours. Ethanolic solution of (0.02%) Sudan Black was spread over the colonies and the plate was kept undisturbed for 30 minutes. They were washed with ethanol (96%) to remove the excess stain from the colonies. The dark blue stained colonies were taken as positives. All the positive isolates were assigned a code and were sub-cultured and maintained in their respective slants at 4◦C

Sub acute :characterized by high and intermittent fever. Above signs can also be seen. Faeces will be dry. Orange mucous covering on faeces. Urine color is dark orange. Petechial hemorrages can also be found on nictitating membrane. Ictrus is also found in both B. eqii and caballi. Putrid smell is also found in urine. Death can also