Potato Enzyme Lab

Enzymes are biological catalysts, which means it decreases activation energy in reactions. The lower activation energy in a reaction, the faster the reaction rate. Many enzymes alter their shape when they bind to the activation site. This is called induced fit, meaning for the enzyme to work to its full potential it has to change shape to binding substrate. The location of enzyme’s activation site is on the surface of the enzyme, where the binding of substrates take place. Enzyme activity can be influenced by a variety of environmental factors. If the concentration of enzyme is low, and there is a great deal of substrate, then increasing enzyme concentration results in more molecules available to convert substrates to products. Thus, increasing enzyme concentration can increase reaction rate. If substrate concentrations are low, and many of the existing enzymes are idle because of a lack of substrate, then adding enzyme will have no effect on reaction rate. Enzyme concentration affects the enzyme activity, because the more enzyme concentration the faster the reaction rate, until it hits it’s limiting factor. When substrate concentration is increased, it also increases rate of reaction. Temperature plays an important

Enzyme catalysis is dependant upon factors such as concentration of enzyme and substrate, temperature and pH. These factors determine the rate of reaction, and an increase in temperature or pH above the optimum will

Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is

Enzymes are specific-type proteins that act as a catalyst by lowering the activation energy of a reaction. Each enzyme binds closely to the substrate; this greatly increases the reaction rate of the bounded substrate. Amylase enzyme, just like any other enzyme, has an optimum PH and temperature range in which it is most active, and in which the substrate binds most easily.

Introduction An enzyme is a catalytic molecule that speeds up reactions to a rate that limits at equilibrium. When a substance is going to have a reaction, it will require a activation energy and the enzyme lower that activation energy. The ability to do this comes from the substrate that the enzyme contains. In the enzyme molecule, there are active sites where it can interact with a substrate and then catalyzes reactions.

“Enzymes are proteins that have catalytic functions” [1], “that speed up or slow down reactions”[2], “indispensable to maintenance and activity of life”[1]. They are each very specific, and will only work when a particular substrate fits in their active site. An active site is “a region on the surface of an enzyme where the substrate binds, and where the reaction occurs”[2].

Enzymes are proteins that either speed up a chemical reaction without being used in a process in other words it is also catalyze (Jacklet 1998). They have different regions on its surface called an active site where it can recognize one or more molecules (Jacklet 1998). Enzymes are the main reason for living cells chemical reactions to stay alive (Jacklet 1998). Substrate chemically attracts the active site to bind and form short lived partnership the enzyme substrate complex (Jacklet 1998). When the reaction has occurred the substrate has

Enzymes are organic catalysts that speed up metabolic reactions (Denniston, 2007). In short, enzyme functions by binding one or more of the reactants in a reaction. Specific enzymes only lower the activation energy for specific reactions and enzymes are shape-specific. The reactants that bind to the enzyme are known as substrates of the enzyme. The exact location on the enzyme where substrate binding takes place is called the active site of the enzyme. When substrates fit perfectly into the enzyme’s active site, the enzyme is able to catalyze the reaction (Denniston, 2007). The activity of enzymes is affected by both the concentrations of enzymes present and the substrate concentrations present. As the number of enzyme upsurges, the enzyme’s reaction rate also rises. Also, as the number of substrate upsurges, the enzyme’s reaction rate initially increases. Enzymes have to be met at an equilibrium in order to work properly and efficiently. These conditions include temperature, the concentration of salt, and the pH level. If the equilibrium for an enzyme was altered, the enzyme may denature, or change its shape, and deactivate. (Richard B. Silverman, 2002). As a result, the enzyme would no longer be able to catalyze the reaction and the reaction rate would significantly decrease

Organisms cannot depend solely on spontaneous reactions for the production of materials because they occur slowly and are not responsive to the organism's needs (Martineau, Dean, et al, Laboratory Manual, 43). In order to speed up the reaction process, cells use enzymes as biological catalysts. Enzymes are able to speed up the reaction through lowering activation energy. Additionally, enzymes facilitate reactions without being consumed (manual,43). Each enzyme acts on a specific molecule or set of molecules referred to as the enzyme's substrate and the results of this reaction are called products (manual 43). As a result, enzymes promote a reaction so that substrates are converted into products on a faster pace (manual 43). Most enzymes are proteins whose structure is determined by its sequence of its amino acids. Enzymes are designed to function the best under physiological conditions of PH and temperature. Any change of these variables that change the conformation of the enzyme will destroy or enhance enzyme activity(manual, 43).

Each enzyme is very specific and can only catalyze a certain reaction. The specific reaction catalyzed by an enzyme depends on the molecular structure and shape of a small area of the enzyme’s surface called the active site. The active site an attract and hold only its specific molecules. The target molecule that the enzyme attracts and acts upon is called the substrate. The substrate and the active site of the molecule must fit together very closely. Sometimes the enzyme changes its shape slightly to bring about the necessary fit.

Enzymes are proteins which acts as catalysts and an active site is an area on the exterior of the enzyme on which the substrates will bind to, and stimulates a chemical reaction. Substrate can only bind to specific enzymes because the active site has a very precise form, in which a specific substrate can bind to. This is referred to as the enzyme-substrate specificity, and can be compared to a lock & key. A substrate (key) can only bind to an enzyme (lock) if they fit. An enzyme inhibiting decreases the reaction of an enzyme catalysed. There are two types of inhibitors; competitive and noncompetitive inhibitor. Competitive inhibitors impedes the substrates from binding to the active site, because the inhibitor and the substrate is structurally

Enzymes are very efficient catalysts for biochemical reactions. They speed up reactions by providing an alternative reaction pathway of lower activation energy. Like all catalysts, enzymes take part in the reaction - that is how they provide an alternative reaction pathway. But they do not undergo permanent changes and so remain unchanged at the end of the reaction. They can only alter the rate of reaction, not the position of the equilibrium. Enzymes are usually highly selective, catalyzing specific reactions only. This specificity is due to the shapes of the enzyme molecules.

An enzyme is a biological catalyst. It speeds up a reaction by lowering the activation energy required to start the reaction. It speeds up a reaction, but remains unchanged unless certain limiting factors are introduced. It is composed of polymers of amino acids. An enzyme has an optimum pH and temperature. When an enzyme is at its optimum conditions, the rate of reaction is the fastest. In their globular structure, one or more polypeptide chains twist and fold, bringing together a small number of amino acids to form the active site, or the location on the enzyme where the substrate binds and the reaction takes place. An enzyme has an active site, which has a unique shape into which only a substrate of the exact same unique shape can fit.

: Enzymes are made up of proteins and their function is mainly determined by their structure. The primary structure of an enzyme is determined by the sequence of amino acids. The secondary structure is based on the folding of the primary structure. Lastly, the tertiary structure is based on the folding of the secondary structure and its interactions of the side chains. The purpose of an enzyme is to speed up chemical reactions in a cell. Temperature is a factor that affects enzyme activity because as temperature increases the enzyme reaction increases too. Also, pH levels also play a role in affecting enzyme activity. Very high or very low pH levels can result in the loss of enzyme activity. For an enzyme to maintain activity it needs to be

There are three factors that will alter an enzyme: temperature, pH and salt. All three will change the structure of the enzyme by denaturation and rendering it useless when a drastic change occurs from levels that are normal. A slight alteration can speed up reaction to certain saturation or slow the process of reaction.