Either the solution is hypotonic which means that the solution has a lower concentration than the potato core and this would cause water to flow into the potato and make it larger and therefore increase its mass. The state the cell is in is called turgid.
Though the theory following the hypothesis is correct and the experiment was carried out with as much attention as possible in a high school laboratory, the results obtained were still indicative of a few errors and did not support the predicted hypothesis. From the results obtained it can be concluded that as the concentration of sucrose increases the average percentage change in mass decreases. This is because the salt concentration inside the potato cubes of 10%, 15% and 20% concentration is less that in the salt concentration on the sucrose solution, thus the three cubes submerged in the 10% - 20% concentrated solution lost mass (hypertonic). However the cube submerged
Trim the skin off with a knife (Try to make them all the same width.)
out the potato and dry it to ensure excess water is not added to the
The purpose of this experiment was to investigate the effect of the change in concentration of sucrose on the rate of osmosis in cylinders of potatoes.
During osmosis, solvents move across a semipermeable membrane in order to regulate the solute balance within the cell (Campbell Biology). Experiment 5.5 was conducted to further research osmotic activity, particularly in potato cells in different osmolarities of a NaCl solution. The goal of the experiment was to find out whether the potato slices used would be hypotonic or hypertonic to the different osmolarities. This process is relevant because without osmosis, the passage of solvents would not be possible. To perform the experiment, seven potato slices were soaked in 5cm of a different osmolarity level of a NaCl solution (0M – 0.6M) to determine whether each slice was hypotonic or hypertonic to the NaCl solution it was placed in, based on percent weight change. The prediction that the potato slices soaked in solutions with lesser osmolarities (0M – 0.1M) of NaCl would be hypotonic to their solutions, and the potato slices soaked in solutions with higher osmolarities (0.2M – 0.6M) would be hypertonic to their solutions was supported by this experiment because the slices soaked in (0.2M – 0.6M) had
My prediction is that as the concentration increases, the potato cell will lose more weight. This is because of the osmosis of water particles from the potato cell cytoplasm to the solution, resulting in a loss of weight. As the concentration decreases, the potato will lose less weight until a certain point where the osmosis of particles in and out of the potato cells will be equal. I also predict that as the salt
2) When the concentration was at 0.3M, the potato’s cytoplasm and the sucrose solution was isotonic. The concentration of the potato’s cytoplasm was having the same solute concentration as the surroundings. Therefore, there would be no net movement of materials happening.
Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is
Van’t Hoff’s Law suggests that the osmotic potential of a cell is proportional to the concentration of solute particles in a solution. The purpose of this experiment was to determine if there are any differences between the osmolalities, the no-weight-changes of osmolalities, and the water potentials of potato cores in different solutions of different solutes. The percent weight change of the potato cores was calculated through a “change in weight” method. The potato core’s weight was measured before and after they were put into different concentrations of a solute for 1.5 hours. In our experiment, there were no significant differences from the osmotic potentials of our results and the osmotic potentials of other scientists work. Ending with chi square values of 2.17 and 2.71, and p values of 0.256 and 0.337, concluding that there is no difference in water potentials of potato cores in different solutions of different solutes at varying concentrations.
This was due to the water moving out of the core’s cells to maintain homeostasis using osmosis (diffusion of water across the cell membrane). The solutions with the concentrations of 0.0 M, 0.1 M, 0.15 M, and 0.2 M were hypotonic (meaning that the concentration of solutes inside the cell was higher than the concentration outside of the cell) so the mass of those potato cores increased since water moved into the potato cores using the process of
If the C.melo is put in the lowest sucrose concentration, then it will cause an increase in mass because the water molecules pass from an area of high water potential, although the water itself, to an are of low water potential in the C.melo. As a result of this, the C.melo in the concentrations of sucrose with a lower water potential will reduce the initial mass as the water potential is higher inside of the C.melo, than in the sugar concentrations. The “net movement” (in the direction of increased concentrations) of the water molecules is into the cell, and the cell will increase in mass.
The Purpose of the Potato Lab was to see how salt water will affect your cells. The example used was, if a person was stranded in the middle of the ocean with no fresh water, would the salt water hydrate them? Since using human cells wasn’t an option, the lab called for potatoes to replicate what would happen to human cells. The potatoes were placed in water beakers, one with salt and one with no salt, to see how the potatoes would be affected by the two different solutions. The two different solutions were hypertonic, and hypotonic. Hypotonic solutions are solutions that have a higher percentage of water molecules to other solutes in the solvent. Hypertonic solutions are solutions that have a lower percentage of solutes in the solvent than water in the solvent. These solutions are a major part in osmosis. Osmosis is a process in which one side of a solution is separated from the other side with a permeable membrane, if one side has a higher concentration of molecules, the molecules will shift over to the side with less molecules to create
In conclusion when the molarity level was at 0 and at .2 the potatoes had gained mass so therefore they were placed in a hypotonic environments. When the molarity level was .4 and above the potatoes loss mass so therefore they were placed in hypertonic environments. So the different in concentrations does change the mass of the potatoes because they determine the osmosis environments.
In this lab we are going to discovery how osmosis works using a semi-impermeable membrane a potato slice. Osmosis is known as the movement of water in and out of a cell. To understand how this works we must understand two terms. Hypotonic means the environment has less solutes compared to the inside of the cell. Hypertonic means that the environment has more solutes compared to the inside of the cell. With osmosis water will always move from hypotonic too hypertonic. So the question is will water move into the potato or out of the potato? Will these results change when placed in different morality of salt water? To calculate these results, we will measure the mass of potatoes cut into equal sizes then soak these potato slices in different morality of NaCl for thirty minutes and measure the mass change in each potato slice.