Pp Lab Report Digestion Essay

1569 Words Mar 23rd, 2015 7 Pages
LABORATORY REPORT
Activity:

Effect of Dietary Fiber on Transit Time and Bile

Name:

Hope Schallert

Instructor:

Dr. Weithop

Date:

03.08.2015

Predictions
Dietary fiber will: increase transit time
Dietary fiber will increase the amount of bile acid in solution

Materials and Methods
Effect of Dietary Fiber on Transit Time
1. Dependent variable. number of radiopaque markers in GI tract
2. Independent variable. amount and type of fiber in meal
3. Controlled variables. calories in meal, nutrients in meal

Effect of Dietary Fiber on Bile Acid Adsorption
1. Dependent variable. amount of bile acids in solution
2. Independent variable. amount of fiber in meal
3. Controlled variables. moisture in meal, size of meal particles, calories and
…show more content…
again I am going to assume this is soluble since I have not done it yet RC42 17 0 LC33 21 4 SC13 17 4Total 83 54 8
8. Which meal had the least percentage of markers in the total colon at 24 hours?
Insoluble fiber-rich diet

Table 5. Effect of Dietary Fiber on Bile Acid Adsorption
Experimental Optical density (OD) of colored filtrate (reflects amount of bile 1 - OD (reflects amount of bile acid adsorbed by food particles)
Trials
acid in filtrate)
Bile Acid
Standard
Standard Fiber-Rich Fiber-rich
Bile Acid
Standard
Standard Fiber-Rich Fiber-rich
With Buffer meal without meal with
Meal
meal with With Buffer meal without meal with
Meal
meal with
Solution (No bile bile acid Without Bile bile acid Solution (No bile bile acid Without Bile bile acid
Fibers)
Fibers)
1
1
0
0.47
0
0.32
0
1
0.53
1
0.68
2
1
0
0.45
0
0.3
0
1
0.55
1
0.70
3
1
0
0.51
0
0.41
0
1
0.49
1
0.59
Average
1
0
0.48
0
0.34
0
1
0.52
1
0.66

Effect of Dietary Fibers on Bile Acid Adsorption

9. Which had the greatest average OD (amount of bile acid in filtrate), the standard meal with bile acid or the fiber-rich meal with bile acid? Standard meal
10. Which had the greatest average 1- OD (amount of bile acid adsorbed to meal particles), the standard meal with bile acid or the fiber-rich meal with bile acid? fiber rich meal

Laboratory Report/ Hope Schallert/ Effect of Dietary Fiber on Transit Time and Bile/ Dr.

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