Preparation of Penicillin

Next, I poured distilled water just below the 250ml mark on the neck of the volumetric flask.

Next, aseptically add 20mL of T-soy broth and 2mL of late log phase of B. thuringiensis culture. Incubate this flask for 24 hours at thirty degrees Celsius shaking at 180rpm. The next time in lab, remove between 1 and 1.5 mL from the top of the tube that holds the soil using a syringe. Open a package of .22μm filter and place the syringe in the top of the filter and dispense the liquid into a centrifuge tube that is labeled as 100 and close the tube immediately. For the negative control, dispense 1mL of SM buffer into a microcentrifuge tube labeled negative control using a syringe. With four microcentrifuge tubes label them -1, -2, -3, and -4. Add 90 μL of SM to each tube, and then add 10μL of the 100 tube to the -1 tube and vortex. Then add 10μL of the -1 tube to the -2 tube and vortex. Keep this process going till the -4 tube. Next, dispense 50μL of the undiluted sample into .5 mL of B. thuringiensis and then vortex. Now mix in 5 mL of TA to the culture tube and pour onto a plate labeled 100 and let solidify. On a different plate, draw a grid with sections labeled negative control, -1, -2, -3, and -4. Aseptically add 4.5mL of TA with .5mL of B. thuringiensis by pouring and then pour onto the plate evenly. Let the plate sit for about ten minutes. After ten minutes transfer 5 μL of the negative control and the dilutions into the proper

To begin with, penicillin was accidentally discovered by Alexander Fleming in 1928. As stated in, “The discovery of penicillin: the true story,” ….. Fleming had gone on a month long vacation, and while away, a stray mold spore came through an open window and landed on one of the many bacterial cultures Fleming had not put away before he left.” The series of events leading to the discovery were not all natural. Fleming had put the cultures in a tray of lysol to soak after succinctly looking over. When a former lab member came to visit Fleming. He showed him some cultures and noticed that one looked

In this reading there was many very interesting facts about penicillin. Penicillin was a breakthrough in the medical field in the 20th century. The scientist Alexander Fleming had accidentally spilled a vile of bacteria on to a plate of bacteria to his benefit he found that is had stopped the growth of bacteria. This accidental discover has benefited everyone in the world because we have to get these shots if we are infected, plus it saved many life’s in world war II. However, Penicillin is considered an antibiotic chemical that is created by living organism to stop the growth of bacteria and prevent an illness if people were to come into contact with this problem. Since the discovery and massive stock piling of this cure it has become less

Fill a test tube about 1/3 full with cold tap water for use in step 34.

room temperature. Vials 4, 5, and 6 should be in the bath containing water that is 10oC. After the

Add RO water to the 25 ml volumetric flask up to the mark. Put stopper on the flask and shake it properly.

Penicillin is a type of antibiotics. It fights nasty bacteria in a human body. Penicillin is used to help many different infections caused by bacteria. Penicillin was made for the soldiers in world war one, it was to help bullet wound infections and infections caused by lost limbs. Penicillin is made from extracting it from mould.

Volume of water This was kept constant by using a 200 ml beaker to fill up the jars used in the experiment. The uncertainty of the volume of the water was: +/- 10 ml

Get a 10.0 mL pipet and suction bulb then use deionized water to rinse the pipet. Then use the rinsed pipet to add 10.0 mL of deionized water to the flasks weighed previously.

2. Fill a flask with pure water (0% sugar), another with 0.2M water, another flask with 0.4 M water, and another flask with 0.8M water.

8. Third week: Remove the F1 flies from the vials and place them into the morgue. The F2 generation are the eggs and /or larvae in the vial. Place the vial in a warm place.

In 1928, after the careless mistake of forgetting to clean his dishes, Alexander Fleming discovered penicillin. The scientific community still celebrates his discovery since it was a major breakthrough in science, especially in the antibiotics and fermentation field. Although his discovery changed various scientific fields and it opened the doors for research in areas not studied or considered relevant before, the actual usefulness of penicillin was truly reached until the large scale production of penicillin was achieved. Before its manufacturing penicillin was considered for researching purposes, but after its manufacturing during World War II, it truly became helpful for humanity because it could finally start performing within its antibiotic spectrum.

After the hour has passed, remove the tubes and add 2-3 drops of biuret reagent to each tube and shake.

1. Number four clean test tubes 1-4. 2. Place 1 ml (or 20 drops) of the following solutions into each tube: water, egg albumin, starch, and chicken broth. 3. Add 3 drops of Buiret reagent to each tube and gently shake