Presentation on Anti-Malaria Mosquitoes

1011 WordsJun 18, 20185 Pages
The Malaria Protozoan parasite, of the genus Plasmodium. There are two main types of Plasmodiumthat infect humans Plasmodium Falciparum Plasmodium Vivax Transmitted by female mosquitoes Develops in mosquito gut Migrates to salivary glands Transfers to other organisms through the saliva of the mosquito. The Mosquito A mosquito is an organism of the family Culicidae. The females require a blood meal to develop eggs. The mosquito vector for malaria is the mosquito genus Anopheles. Transfers Plasmodiumthrough saliva while feeding on blood. Malaria, mosquitoes, and humans A Mosquito-borne…show more content…
In this test, I will be using Plasmodium Berghi, a type of malaria that is only infectious to mice. Materials Pipetmen Anopheles stephensi mosquitoes, both eggs and live mosquitoes Mice Anti-Pbs21 gene in plasmids Microinjector Lots and lots of eppendorf tubes Microscope Airtight habitat for mosquitoes Random assorted materials Methods Microinjection Inserting plasmid into mosquito egg Bacterial transformation Inserting plasmid into Asaia Inoculation Infecting mosquitoes with transformed Asaia Procedure Transgenic mosquito Take plasmids containing anti-Pbs21 gene and insert them into mosquito eggs using a microinjector. Repeat several times to increase the chances of getting a successful mosquito. Grow the mosquito to maturity. Asaia-infected mosquito Take Asaiabacteria and put it with plasmids containing the anti-Pbs21 gene and the gene for GFP Heat shock the bacteria to make them accept the plasmids. Plate the bacteria and select the colonies that glow in the UV light Infect adult mosquitoes with the successful bacteria. Procedure Ctd... Both types of mosquito Take both types of mosquito, as well as normal mosquitoes as a control, and set them up in exactly equal habitats. Evaluate each generation of mosquito on Percentage of ones with immunity to malaria Lifespan Predicted Results I
Open Document