Procedures for Testicular Biopsy

656 WordsFeb 17, 20183 Pages
Biopsy procedure Samples were blinded for the ICSI laboratory examiner. For all patients, open surgery biopsy was applied to retrieve specimen of sperm. At the beginning of open biopsy, the skin over the testicle was cleaned with a germ-killing (antiseptic) medicine. The area around covered with a sterile towel. (As like what was done in onset of local anesthesia.) The testis was grasped between the thumb and forefinger of the non-dominant hand. An incision was then made over the same area immediately. The size of the incision was about over 0.5– 1cm . An vascular area close to midportion of medial, lateral or anterior surface of the testis was chosen, tunica albuginea incised longitudinally with a 150 ultrasharp knife. The testicular parenchyma (approximately 50-750 mg) was excised with sharp, curved scissors and placed in temperature-adopted Hams F10 culture medium supplemented with 10% Plasmanate. Wet preparations transferred to the in vitro fertilization (IVF) lab. Individual tubules were isolated by immediate initial dispersal of the specimen with two sterile glass slides. Mincing of the tissue with a sterile scissors in the Hams F10 medium additional allowed for mechanical dispersal and preparing sequential passes of tissue suspension (detection of fully condensed spermatids) through a 24-gauge angiocatheter. As soon as spermatozoa were identified, no further testicular incisions are made. To prevent devascularization of the testis from damage to the subtunical
Open Document