Project: Streak Technique

After each technique was used, there were only results for the streak plate and the spread plate. No results were found for the pour plate. The bacteria can be counted for the streak plate, but not for the spread plate. It was found that using the streak plate technique, it would be easier to count the individual colonies. It was also discovered that doing the serial dilution to make sure that the agar is cooled before adding the bacteria.

Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium.

Introduction: This experiment was centered on metabolic and biochemical testing procedures. The rationale of performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample.

There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods. This experiment was done by applying methods in order to identify an unknown bacterium.

The culture changes to yellow in the presence of acids indicating a positive test 11. An important tool available in the Virtual Unknown program is the Identification Matrix. From the portion of the identification matrix shown in the Identifying Bacteria tutorial, identify at least one bacterium that has a positive result to the arabinose fermentation test. (1 pt)

BLAST screen shot 3. Analysis or troubleshooting According to my DNA analysis when inputted into the BLAST system my unknown bacteria has an eighty-six percent similarity to Micrococcus Luteus. These result deviate a bit from the results obtained in the Gideon experiment. Since, my DNA sequence result was not as high as I would have preferred this could be due to the limited amount of DNA concentration available after the PCR experiment was completed. A factor that could have allowed me to get a different bacteria species from the biochemical test could have been that the sample taken from the culture could have been contaminated. If the bacteria were not cultured properly this could also affect the results. Making a mistake during the PCR experiment

1. Title: The Process of Determining the Unknown Bacteria #9 Rachel Judecki July 5, 2011 2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

Cody Spenillo Professor VJ Rossmann Unknown Organism Assignment My unknown organism #11 is Escherichia coli (E. coli). E. coli belongs to the family Enterobacteriaceae. E. coli is Gram-negative, facultative anaerobe, and rod-shaped bacteria (bacillus). [1]

In case of Sulfur Indole Motility test, unknown was negative for sulfur reduction since there was no color change and the media was not black indication no sulfur was reduced. For indole production test, red color was noticed at the top when the Kovac’s reagent was added indicating tryptophan was broken into indole and pyruvate. The motility test was positive since there was growth radiating outward from the stab indicating motility.

Unknown Organism AA Introduction: The main objective was to identify an unknown organism by utilizing skills we learned in our labs this semester. The purpose was to attain the possible identity of the unknown organism by actually performing biochemical tests and staining techniques we learned in lab. After performing and analyzing the results, we were able to use Bergey’s Manual of Systemic Bacteriology as a guideline to narrow down the genus of our organism test by test.

PROCEDURE - These procedures were followed This microbe was identified from colonies appearing on both plates. Gram stain results: gram negative rods. Acid from glucose: positive. Gas from glucose: positive. Lactose fermentation: negative. Sucrose fermentation: positive. DISCUSSION - Carbohydrate testing utilizes the different ways that bacteria metabolize different sugars by inoculating different broths with the test bacteria and seeing if there is a change in acidity and/or if any gases are produced. For glucose testing, we would check to see if gas is produced and if the ph of the broth solution drops for a positive test result. For lactose, we check to see if the ph drops or becomes more acidic. For sucrose we check the same was as for lactose but use a sucrose solution instead. A negative result would mean that the solution contained a base/alkali ph.

In class, we were given the task of identifying an unknown bacterium broth culture. After receiving number 69, I went through several tests to figure out what bacterium I received. First, I created a slide from my broth by putting a small amount of the unknown broth on to a

Mannitol Salt Agar (MSA) differentiates halophile bacteria, and bacteria’s ability to ferment mannitol. Fermenting mannitol results with a yellow halo around colony from the acid produced (staphylococcal species). MSA is composed of enzymatic digest of casein and animal tissue (nitrogen, vitamin carbon source), D mannitol (carbohydrate), NaCl (7.5%, halophiles), phenol

On the first day of this exercise, after using apsetic technique I inoculated a CNA .I moistened the swab with sterile water and before picking up a sample of bacteria, I taped the bottom of the broth against the palm of my hand. I dipped the swab into broth culture labeled #2 and smeared the entire surfaced of the CNA plate, I then turned the plate and swabbed the plate in a perpendicular direction to the first streaking creating a lawn. I used a T-soy plate to perform a streak isolation. Using apsetic technique and a loop, I obtained a sample of bacteria from the test tube #2, smeared the bacteria in a vertical smear, then streaked through the fist smear 10 times from the edge of the plate inwards, repeated the same procedure 4 times

Discussion- The conclusion that was made from this experiment was that as the streaking is completed the consistency as well as the concentration degrees allowing for a more accurate identification of the individual particles. The results lead me to this conclusion because as the streaks were completed the