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Propolis Lab Report

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Purpose; The antioxidant properties of Capparis spinosa honey and propolis and the effect of combined honey and propolis administration on urine volume and electrolytes were studied in rats.
Material and methods; Capparis spinosa honey (1g/kg.b.wt), propolis (100 mg/kg.b.wt), honey/propolis mixture (100 mg/1g/kg.b.wt), distilled water (1 ml/kg.b.wt) and furosemide (10 mg/kg.b.wt) were orally administered to five groups of rats for 21 days. Urine volume, blood and urine sodium, potassium and chloride were measured. The antioxidant activity of propolis and the honey was assessed and their total phenols and flavonoids were determined.
Results; Propolis and Capparis spinosa honey contain polyphenols including flavonoids and propolis demonstrated higher antioxidant activities than honey. Honey significantly increased urine volume and urine electrolyte excretion. Propolis has no significant effect on urine volume but co-administration of propolis and honey caused significant diuresis. No major changes were observed in plasma electrolytes with use of honey, propolis or their combination.
Conclusion; Honey and propolis have antioxidant activity and contain polyphenols including flavonoids that were more pronounced in propolis. Honey has a significant diuretic activity alone or in combination with propolis. This is the first study comparing the diuretic effect of co-administration of propolis and Capparis spinosa honey with furosemide.
Key Words;
Honey; propolis; antioxidants;
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