Proposed Reasons For The Growth Of Microcter And Bacterial Growth

There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium.

In the beginning of lab, we were advised to obtain a nutrient agar petri plate, which is used for the cultivation of microbes supporting growth of non-fastidious organisms. Since it contains many nutrients, a wide variety of bacteria and fungi can grow. Taking the plate,

No growths that were unexpected developed. I.e. if it is a fluid base growth there should be no air born bacteria in the culture. No mold is another way to know no contamination occurred.

According to my DNA analysis when inputted into the BLAST system my unknown bacteria has an eighty-six percent similarity to Micrococcus Luteus. These result deviate a bit from the results obtained in the Gideon experiment. Since, my DNA sequence result was not as high as I would have preferred this could be due to the limited amount of DNA concentration available after the PCR experiment was completed. A factor that could have allowed me to get a different bacteria species from the biochemical test could have been that the sample taken from the culture could have been contaminated. If the bacteria were not cultured properly this could also affect the results. Making a mistake during the PCR experiment

How can you interpret this? For example, in my dish with the sink and shoe, there were no traces of bacteria on the shoe side but a lot of bacteria on the sink side. If the bacteria were to have multiplied and spread due to being in the same dish, it would have given me a positive result on the shoe side which it didn’t.

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.

|EMB Agar | |Distinguishes bacteria that ferment |Dark blue colonies with|E. coli and P. |

Many tests were completed on the unknown such as gram staining and inspection under microscopes to find whether the bacterium is gram positive or gram negative. Chemical resistance tests were also performed to see if certain chemicals affected the unknown growth or if it didn’t affect the bacteria at all. Each biochemical test

A wide variety of processes had been performed to determine what culture had been acquired in class. My group had acquired culture tube unknown #3. We first isolated the bacteria. In this step we took the broth of the unknown #3 and grew it on an agar plate. The first process that had been performed was discontinuous streaking, and continuous streaking to grow the culture for future use in the lab and to have extra to perform a whole variety of test to determine the unknown. The next experiment that had been performed

In class, we were given the task of identifying an unknown bacterium broth culture. After receiving number 69, I went through several tests to figure out what bacterium I received. First, I created a slide from my broth by putting a small amount of the unknown broth on to a clean slide and letting it dry for ten minutes. After this, I stained the slide by applying four reagents in order; crystal violet, grams iodine, decolorizer and safranin. From the stained slide, I discovered that this bacterium was gram-negative, which would determine the next couple of tests I would do to identify my unknown bacterium. I began by streaking for confluent growth from my broth culture onto a TSA plate. From the TSA plate, I aseptically transferred a loop

that the bacteria are capable of growing on the agar and that there was nothing

The result of this experient shows that lunch meat grows more bacteria than Skittle and bacteria does grow more if we drop it. In the result of lunch meat, tile floor grows the most bacteria and for the Skittle, bacteria grows more on the cotton cloth. During the experient, I find out that it’s interesting that after 12 hours, that control sample for lunch meat grows more bacteria than the lunch meat on cotton cloth.

As shown in the above results finding a phage is not guaranteed. Even when using multiple different soil samples and different protocol to isolate a phage there is still chance you won’t find a phage. There were no phages present within any of the plates there are a lot of reasons why this might be. The main reason is probably because of possible errors such as removing from the aseptic zone which would prevent contamination or having improper techniques for top agar pouring which could also screw the data. A lot of the soil samples collected were very dense and when we got our enrichment medium soil mixture back from the shakers we did notice that our soil samples had not separated as much as other groups. This could have caused contamination

It is common knowledge that germs are everywhere. Often, most germs pose no risk to people and when they do, such infections can be treated with antibiotics. However, some bacteria can learn to resist these infection killing drugs and become “superbugs”. This evolutionary process is normally invisible, but now scientist can watch it happen. A microbiologist at the Georgia Institute of Technology, called Sam Brown, placed bacteria in a liquid growth medium. The germs grew, and then the scientists added an antibiotic. They then could tell which germs were immune, because they would greatly outnumber the other germs in the flask. Contrary to Brown’s method of experimentation, Michael Baym, a Harvard Medical School student stated that Brown’s method were not conducive to real world situations. Instead, Baym placed the germs in a MEGA plate which was then examined under a powerful microscope. Baym then repeated a similar process to Brown and could plainly see the bacteria mutate and spread throughout the sample. He wants to use this method to apply his conclusion to different situations and see how the bacteria

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a