Protein Adsorption Using Fluorescently Tagged Rh Fgn

EXPERIMENT 3 Surface Tension of n-Butanol and Amount Adsorbed Richard Sawatzky [104230213] 03-59-240-61 TA: Ghazale Gholami University of Windsor Abstract The purpose of this experiment is to determine the surface tension of n-Butanol by measuring the amount of n-butanol absorbed in a graduated capillary tube, at different concentrations. This is done by finding the h values for the various concentrations and determining the radius of the capillary tube using the h of water. We found the radius to be 0.026 cm and then using this value along with h of the concentrations, we found the surface tension of 0.8, 0.6, 0.4, 0.2, and 0.1 M of n-butanol which were 23.6, 36.4, 49.1, 55.17 and 63.1 dyne/cm.

When faced with a crisis of identity, we often stick with those who care in the best position to support us. In Neil Smith’s “Green Fluorescent Protein,” When Max was fighting with Ruby-Doo; he wants to stay with his mom for comfort. GFP is a metaphor for Max’s realization that he is queer. Max is ensuring of his feelings towards Ruby-doo. “In Pop’s arms, the white guinea pig turns a brilliant green…… It’s very weird and really scary. [it’s] kind of beautiful in an unexpected way” [50]. He tried to say same sex love can be weird and scary, but it’s love and love it a beautiful thing. Family plays an important role in max’s life. This is what for example Max’s said “I want to say, don’t go. Not that I’m afraid her date

Gelatin is observed to have a lower absorbance reading than lysozyme in only the Bradford assay, while in the BCA assay gelatin was observed to have about the same absorbance as lysozyme. In Bradford assay, the color yield for proteins with higher content of tryptophan, tyrosine, or cysteine residue, will be higher. The observed protein sensitivities did not correlates with the amino acid content in the Bradford assay. As presented in Table I, the mole fractions of lysine and arginine in BSA were 10 mole percent and 4 mole percent, respectively. The sensitivity should have been higher in BSA than in the Bradford assy. However, the observed sensitivities did correlates with the amino acid contents in the BCA assay.

More apparent on hydrophobic fibers because their fibers have greater electrical static attraction for each other ran do not fall off the fabric surface. Wool also pills because it has a scaly surface.

2. UV treatment increased the mechanical strength of the fibrin microthreads, decreased the swelling ratio, and decreased cell proliferation. UV treatment increased the mechanical strength of the threads the most at around 17 J/cm^2, and the UTS of the threads increased after treatment. Swelling ratio of the threads decreased from around four times more than dry threads to around 2.5 times more after UV treatment. Cell proliferation decreased because while the number of cells on non UV treated threads continued to

The specificity of albumin binding experiment was to determine the binding interactions that occur between serum albumin and three synthetic dyes with the use of electrophoretic procedure. Whole blood, or plasma. Clots upon standing and if the clot is removed, the remaining straw colored fluid is called serum. The major protein in serum is albumin which functions as a carrier molecule for the transport of certain small molecular weight compounds in blood. Molecules that bind to serum albumin are fatty acids, hormones and some synthetic dyes. In this experiment the synthetic dyes used are Bromophenol Blue, Ponceau S and Orange G. we observed that free dyes not bound to albumin migrate faster that albumin or dyes bound to albumin. This

Would the GFP gene in a pGLO transformed E. coli be activated by a sugar other than arabinose?

Literature Review: Research into this topic shows that it is not as simplistic as might be imagined, but has in fact been examined as part of rigorous research projects undertaken on behalf of paper towel manufacturers (Beuther et al, 2010). The ways in which paper towels absorb, and therefore the rates at which they are able to absorb, vary depending on the microscopic fiber structures within individual paper towels, which of course vary greatly depending on the manufacturing materials and processes in the making of each brand of paper towels (Beuther et al, 2010). Fiber types and construction patterns have also been demonstrated to have a large impact on absorbency in natural and synthetic textiles examined in a similar experiment (Beskisiz et al, 2009). Both of these pieces of research suggest certain controls for the paper towel experiment described herein (Beuther et al, 2010; Bekisiz et al, 2009).

Benoit, Pruitt, and Thrall (2009) assessed the quality of wet gauze compared to Superflab. Using different sizes and levels of wetness, and variable densities of the bolus material, the investigators measured each material three times using a 6 MV radiation source. The results were that a wet gauze with a density of 1.02 g/cm3 had the identical measurement to Superflab. While a smaller gauze with a density of .75 g/cm3 was not as efficient. Also, wet gauze eliminated air gaps with the skin surface. The researchers concluded Superflab was least effective in eliminating air gaps and wet gauze with a

During this lab, we investigated the relationship between the surface and the appearance of the blood drop. The thinner the material of the surface, the more the blood drop absorbed into the paper. The height of the drop and the angle of impact were also contributing factors. The diameter of a drop increased with the height of the drop. In addition, we found that when a blood drop hits the surface at a 90 degree angle, a circular stain is produced. As the degree of impact decrease, the blood drops begin to trail towards the bottom of the

. The 3-D tertiary structure of polypeptide proteins globular and is the result of interactions that occur between R groups. Tertiary structure is a result of the bonds between sidechains of amino acids, the R groups. The structure and bonds involve alpha helices, beta pleated sheets, and also regions unique to each protein. Tertiary proteins are held together by four different types of forces; hydrogen bonds, hydrophobic interactions (including Van der Waals interactions), ionic bonding (electrostatic interactions), and disulfide bridges (strong covalent bonds). Hydrogen bonds occur within and between polypeptide chains and the aqueous environment. Hydrogen bonding forms between a highly electronegative oxygen atom or a nitrogen atom and a hydrogen atom attached to another oxygen atom or a nitrogen atom. This links the amino acid

Limitations: Some conditions sample size n=1. No 30min 10mM paper treatment or 60 min 10mM drip treatment from which to analyse impact of duration. No fluorescence.

NASA is constantly looking for ways to attach objects in space, for example; solar panels, fuel tanks, insulation blankets. This gecko inspired technology offers a solution to their problems. These new fibrillar microstructures enable attachment and detachment with a relatively low amount of force, a huge contrast from conventional methods. This project, if successful, will help NASA reduce the number of man hours designated minor repairs and maintenance upon the space station. Consequently, this will allow them to designate more time to the study of Earth, life on Mars and other initiatives. Gecko-inspired tissue adhesives made from a biocompatible and biodegradable elastomer can act as a medical adhesive, replacing structured and assisting in the healing of wounds [1]. The project will also give other students at Arizona State University the ability to use the testing chamber for temperature dependent research purposes, for example: testing biomaterials, bio-inspired materials, analysis of topographical patches for drug delivery and the analysis of cell cultures under specific temperature and

And brown paper was used to detect any lipids found in the sample. After drying, the towel becomes opaque as a negative control and translucent for the positive control.

Proteins can also be fractionally precipitated by adjusting the pH, temperature and dielectric constant. Variations in pH also change the state of ionization of the functional groups, and hence, the net charge of the protein. Usually, the protein’s solubility is at its least at the isoelectric point pI and increases on either side of the pI. At the pI, the net charge of the protein is zero and the protein molecules do not repel each other. As a result, protein-protein interactions are increased and the solubility is at its lowest. (Boyer, 2000)