Proteus Research Paper

The Unknown Bacteria 36/Bacteria # 2 on a TSA plate was examined by the naked eye and under a dissecting microscope. Bacteria # 2 was approximately 3 - 4 mm in diameter. They were circular in form with an entire margin and a flat elevation. The colonies were rough (granular), translucent, and white brownish color with black granules. The Gram stain resulted in a Gram negative rod. After the Gram stain was completed, the bacteria were streaked on an Eosin -Methylene Blue Agar plate and an Enterotube II was inoculated.

The first result of importance was the result of the Gram stain. The observations of the unknown bacteria from the slant culture after Gram staining showed that the unknown bacteria were Gram negative bacilli (Image 1). After determining the unknown bacteria was Gram negative, an oxidase test was conducted on a sample from the slant culture. The cotton swap with the sample of bacteria did not change color when the oxidase reagent was applied, thus providing a negative result. With a negative oxidase test, further tests were conducted to determine various characteristics of the unknown bacteria. A MR-VP broth was inoculated with a sample from a slant culture of unknown bacteria. After incubation, the methyl red reagent was added to the broth, and the broth turned red, providing a positive result (Image 2). An EMB agar streak plate was inoculated with a sample from a slant culture of the unknown bacteria, and after incubation, growth was found on the plate, providing a positive result (Image 3). A Citrate agar slant was inoculated, and after incubation, growth was found on the media, providing a positive result (Image 4). A Urea agar slant was inoculated, and after incubation, the agar had changed from a peach color to a bright pink color, providing a positive result (Image 5). Using the flowchart (Figure 1) developed from the Table of Expected Results, the lab partners started at the oxidase test. Given the negative result of the oxidase test, the flowchart is

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible

This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.

Bacteria are ubiquitous; they can be found on the skin, in the soil, and inside the body. Because of the very nature of this ubiquity, it is important to be able to determine between different strains of bacteria. An example of this is determining the causative agent for a disease so that the patient will be treated with the appropriate antibiotics. It may be important to determine the bacteria in a certain region, because like with enteric bacteria, it is normal to find them in the digestive tract as they are in a symbiotic relationship with our bodies in this area; however, they also cause opportunistic infections in places outside of the digestive tract to our detriment, such as with a urinary tract infection. Some strains of bacteria are common to nosocomial infections, and identifying these bacteria as such helps create the guidelines for healthcare workers in antiseptic technique. All of the morphology and characteristics of each strain of bacteria help us to better understand the role of bacteria in the body as well as helps us understand how they can cause illness, and what treatment regimen to set in place. In lab this semester, a sample of unknown

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

The bases of this experiment was to discover the identify of the unknown from three possible specimens: Klebsiella pneumonia, Escherichia coli, and Enterobacter aerogenes. Utilizaing the T streak technique, the bacteria was isolated into pure colonies for further study. The Gram Stain method was used to identity the morhphology of the bacteria such as the shape and whether the bacteria was Gram positive or Gram negative. Biochemical test were also used to help identify the unknown bacteria. The biochemical test used was the Triple Sugar Iron Agar, Sulfur Indole Motility test, Methyl Red test, Voges-Proskauer test, Citrate test, Urease test, and the Gelatin test. After observing the morphology of the bacteria using the Gram Stain method and utilizing all the possible biochemical test, the bacteria was identified to be Enterobacter aerogenes.

While Proteus vulgaris is not likely to cause any problems to a healthy adult, this bacterium can still cause infections. According to eol.org, Proteus vulgaris is “grouped with the Enterobacteriaceae and is an opportunistic pathogen of humans.” For that reason, it is a bacterium that is known to create UTI’s and wound

A broth in test tube labeled #2 was used to identify the unknown organisms. A T-soy agar plate was the first media I inoculated. I used the streak isolation technique to isolate a pure strain from colonies, so that organisms can be identified. I inoculated a MacConckey to help me identify my Gram negative organism and observed that the organism was a non-lactose fermenter, then I performed a Citrase test which was negative. The last test I performed was a TSI, the butt was positive for hydrogen sulfide production and fermentation of glucose. All 3 results helped me conclude that my Gram negative organism was Proteus vulgaris. P vulgaris inhabits the human intestinal tract of human and animals. It is also found in soil, polluted water and fecal matter. This bacterium is

In the article, Sammy Soshi has been tasked with selecting a new name for Soshi Sumshin Ltd’s electronics department. Sammy has determined there are three different alternatives. The first option is to keep the family name, Sumshin. The second alternative is to buy out a preexisting American brand, Monarch that sold radios in the 1940s. The last possibility is to create a new name.

The genetic mutation that I am doing is called Proteus Syndrome. Proteus Syndrome is a highly variable and is named after Greek-god Proteus who could change his shape. Only a few more than 200 cases have been confirmed worldwide with estimates that about 120 people are currently alive with the condition. Those most readily diagnosed are also the most severely disfigured bones, muscles, fatty tissues, and blood and lymphatic vessels. Proteus syndrome is an overgrowth disorder caused by a rare genetic mosaicism.

Proteus is a Gram-negative bacteria associating to the Enterobacteriaceae family. which is distinct from another species by swarming in the surface of agar (23). Proteus is a broad distribution in the environment that puts up a portion of the normal flora in a gastrointestinal tract of the human. It is ranked third in a cause of hospital-acquired infections(42).The opportunistic human pathogens represented by three species: P. vulgaris, P. mirabilis, and P. penneri

There are many risks available with edible devices since we swallow them and take them inside to our body. Some of them are, what is long term impact of electronic communication through the body? How a device maintain patient compliance? What is the environmental impact of devices that may accumulate in wastewater treatment facility? Many of these questions have being answered in part by successful clinical testing of devices such as the pillCam and the Proteus Discover. One of the most significant risk elements is device retention within the GI tract[11].The risk associate with the device will depend on physical properties of the device and the disease state of the GI tract. Most devices have rigid polymeric coating to protect the electronic

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.