Pseudomonas Is A Key Opportunistic. Pathogen Characterized By High Level Antibiotic Resistance And Biofilm Formation

2284 Words Sep 16th, 2016 10 Pages
Pseudomonas aeruginosa is a key opportunistic pathogen characterized by high-level antibiotic resistance and biofilm formation (1).Biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix adherent to an inert or living surface. Biofilmproducing organisms are more antimicrobial resistant than organisms without biofilm. In some extreme cases, the concentrations of antimicrobials required to kill biofilm positive organisms can be three- to four-fold higher than for biofilm negative bacteria, depending on the species and drug combination (2).
Biofilms have great importance for public health as they are the main cause of nosocomial infections, especially implant-based and chronic infections (3).
Antibiotic resistance in biofilms is due to a combination of many factors that act together to result in a level of resistance that is much higher than that of planktonic bacteria (4,5).
One factor of these is the altered expression of specific genes involved in the biofilm. Some of these genes may function to protect biofilm cells from antibiotics. NdvB gene, encodes the glucosyltransferase enzyme that responsible for the formation of cyclic glucans (6). The glucans are cyclic polymers of 12 to 15 β- (1 →
3) -linked glucose molecules with phosphoglycerol substitutions (7). Inactivation of ndvB gene blocked glucan synthesis without affect the kinetics of biofilm formation, the architecture of the biofilms or growth of bacteria (6). However, ndvB…
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