Pseudomonas Research Paper

3. Mrs. Ganer's class visited the petting zoo, could they also have become sick from microorganisms?

An investigation was necessary to find the cause of the outbreak, and how to stop it from continuing to spread. The campus clinic was interested in testing the following nine patients: Sue, Jill, Anthony, Wanda, Maggie, Maria, Arnie, Marco, and Alvin. All of the students have similar symptoms and agreed to being tested except for Alvin.

Mr. Gawande starts his literature on washing hands. He introduces two friends a microbiologist and an infectious disease specialist. Both work hard and diligently against the spread of diseases just like Semmelweis who is mentioned in the chapter. Something I learned, that not many realize, is that each year two million people acquire an infection while they are in the hospital. Mainly because the clinicians only wash their hands one-third to one-half as many times as they should. Semmelweis, mentioned earlier, concluded in 1847 that doctors themselves were to blame for childbed fever, which was the leading cause of

Pseudomonas aeruginosa is a versatile gram negative bacterium that grows in soil, marshes, and coastal marine habitats, as well as on plant and animal tissues. People with cystic fibrosis, burn victims, individuals with cancer, and persons infected with HIV are particularly at risk of disease resulting from Pseudomonas aeruginosa.

P. aeruginosa is a ubiquitous Gram-negative bacterium that thrives in moist environmental reservoirs such as in the soil, water and plants8, 9. P. aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals, lending to its association with life-threatening illnesses10. In addition to pulmonary infections in CF patients, P. aeruginosa is frequently found in nosocomial infections11. As such, P. aeruginosa is recognized for its medical importance in clinical infections.

also ended up dying and no treatment was given to infected adults and children. The primary document

In this experiment, an unknown bacterium was given to each individual student. The main purpose of this lab was to identify the given unknown bacteria going through a series of biochemical tests as one of the gram negative bacteria among six different Gram negative bacteria Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Salmonella typhimurium. At the very beginning, streaking method; T-streak technique was used to isolate the pure colonies. For the morphological identification of unknown bacteria, Gram Stain Method was done. Biochemical tests that were conducted for the experiment

breeding ground for bacteria to grow and find new pure hosts. Once one man got sick, they all

It has been identified the highest numbered of reported cases were in infants and children up to 14 years of age5. The most common of all disease was campylobacteriosis which was approximately 69% of cases being reported, followed by cryptosporidiosis which was approximately 115 of cases being reported, salmonellosis which was approximately 8% of cases, and verotoxin producing E. coli which was about 5% of the cases

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

At first I met Leslie Hernandez, the Health Planner, who directed me to Shoua Shinde, the epidemiologist and "department detective", whose areas of expertise were biology, microbiology, biostatistics, and knowledge of communicable diseases ("Outbreak at Watersedge," 2004). She informed me that there were many people in the hospital with severe diarrhea and other symptoms and asked for my help in order to understand what is going on. She introduced me to Terry Tower, the public health nurse, who offered me to analyze and map the results of the interview for the first five patients. After mapping the results of five interviews I pinpointed a place to start, which was the Thompson Park, because all five people aside from the other activities and places went there on the same day to participate in Thompson Park cleanup. Then Shoua asked me to go to the park, to explore and take notes of what I think could be important. Meanwhile the number of reported cases increased to 20. In the park I met Mai, the Environmental Health Specialist, whose areas of expertise was toxicology, biology, chemistry, water

The bacteria that was contained within Unknown tube #12 is believed to be Pseudomonas aeruginosa, Figure 1. The bacteria tested to be Gram Stain negative, producing a pink, red color retained from the staining process. When the species of bacteria was plated on nutrient media, the cells produced an irregular and spreading configuration as shown in Figure 2. This same plating test provided the margins and elevation, lobate and hilly, respectively. The specimen was stabbed in a Fluid Thioglycollate Medium (FTM) tube using an inoculated loop of the bacteria. The results of this experimentation indicate the type of oxygen requirement of the bacteria. The test found the bacteria to be aerobic as colonies of the bacteria began to form along the top of the FTM tube (Manual 2017).

Neonatal sepsis is potentially fatal blood stream infection occurring in the first month of life (1). It causes long duration of hospital stay in neonatal intensive care unit (NICU) and associated with additional expenses, morbidity and long term adverse outcome.(2)

Blood culture is the gold standard diagnostic method of neonatal sepsis. However, technical problems as insufficient blood sample or maternal use of antibiotics can be an obstacle in isolating the responsible pathogens.

Listeriosis is a serious infection that result from food contamination with the bacterium L. monocyogenes. It I known to be in fact, “one of the most deadly bacterial infections currently known- with a mean mortality rate in humans of 20 to 30% or higher despite early antibiotic treatment” 11. Besides the transmission of foodborne, it can also be transmitted from mother to fetus or human-to human physical interaction. A mother can consume contaminated food or liquid such as milk during pregnancy and be able to transmit to the fetus through the maternal blood stream or from a colonized genital tract5 (See Figure 3). This can cause serious factors leading to premature delivery, miscarriage, or other serious health issues for the newborn.5