Pulse Chase Experiment

Translation is a task that makes ribosomes synthesize proteins utilizing mRNA transcript made during transcription. In the begining of this task mRNA attaches it self to a ribosome so that it can be reveal a codon (three nucleotides).

3) As a ribosome moves along the mRNA, the genetic message is translated into a protein with a specific amino acid sequence.

Transcription is the formation of an RNA strand from a DNA template within the nucleus of a cell. There are four nucleotides of DNA. These are adenine, cytosine, guanine and thymine. These nucleotides are transcribed to form messenger ribonucleic acid (mRNA) consisting of nucleotides made of adenine, cytosine, guanine and uracil. This transcription from DNA to mRNA happens by an RNA polymerase II. This newly created mRNA is read in the 5' to 3' direction in sets of 3. These sets are called codons. Each mRNA also has a cap and end. On the 5 prime side is a methylated guanine triphosphate and on the 3 prime is a poly A tail. Messenger RNA then moves to the cells cytoplasm and through the cells ribosomes for translation. Messenger RNA is matched to molecules of transfer RNA (tRNA) in the ribosomes to create amino acids. These amino acids subsequently form an amino acid chain. (Osuri, 2003) A visual representation of this can been viewed in figure 3.

For example, for trials one and four, trial one had a greater slope and a greater y-intercept. By finding the velocity we were also able to locate when the object was accelerating and decelerating.

After the DNA has been turned into mRNA a process called translation occurs and it turns the mRNA into tRNA.

When he started the presentation he gave a quick introduction then outlined what he did. Instead of going into every procedure in his experiment he focused on the largest, and possibly the most interesting, portion which was the 16S RNA assay. As he described this relatively complex topic he used

Actinobacteria belong to a diverse group of freshwater and terrestrial microorganisms. Although common, the metabolism and genetics of numerous actinobacteria species are not well understood. The Traxler lab seeks to characterize and define actinobacterial behavior, specifically in terrestrial environments by employing a variety of methods. One approach studies the behavior of Streptomyces coelicolor, a model soil-dwelling actinobacteria, to examine the genetic control of cell fate. Since the genes involved in creating cell structures like vegetative mycelia, aerial mycelia, and spores are not well defined, the Traxler lab seeks to understand how S. coelicolor regulates its gene expression to achieve these complex morphologies. In addition

The interference of RNA and ME in the Bradford assay was compensated by using the blank, because the difference between the addition of the protein and the non-protein blank

The second part of my breaching experiment made me think if it was a real life scenario, and if my phone rang, whether I could trust someone to tell me. Nevertheless the person who informed me that my phone was ringing was a friend I met last year, which made me think of what would have happened if he were not in the room.

The nucleus creates mRNA and the mRNA goes through the nuclear pores into the cytoplasm. The mRNA is transcribed the from the DNA. Once the mRNA enters the cytoplasm it is translated by ribosomes, than the corresponding amino acids come and form proteins.

Protein synthesis is one of the most fundamental biological processes. To start off, a protein is made in a ribosome. There are many cellular mechanisms involved with protein synthesis. Before the process of protein synthesis can be described, a person must know what proteins are made out of. There are four basic levels of protein organization. The first is primary structure, followed by secondary structure, then tertiary structure, and the last level is quaternary structure. Once someone understands the makeup of a protein, they can then begin to learn how elements can combine and go from genes to protein. There are two main processes that occur during protein synthesis, or peptide formation. One is transcription and

Protein synthesis is a two-part process that involves a second type of nucleic acid along with DNA. This second type of nucleic acid is RNA, ribonucleic acid.

Hergenrather, K. C., Rhodes, S. D., & Clark, G. (June 01, 2006). Windows to Work: Exploring Employment–Seeking Behaviors of Persons with HIV/AIDS Through Photovoice. Aids Education and Prevention, 18, 3, 243-258.

It is not unusual to encounter the term managing and coaching used in place of one another. Those who understand the difference might cringe a bit inside when this occurs because it is likely they have worked for, or with, a manager who certainly was not a coach, and consequently ineffective. More succinctly, coaching is a function of managing that every respectable leader of others must be able to do well.

There are certain psychoanalytic concepts expressed by Sigmund Freud that can be applied to interpret literary texts. Most of the literary texts, like dreams, articulate the secret unconscious desires and concerns of the author, that a literary work is expression of the author’s own neuroses. For instance, one may analyze a particular character within a literary work from the psychoanalytic view, but it is usually assumed that all such characters are outcrops of the author’s psyche.