Quantitative Estimation of Sugars to Soft Drinks

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Biology IA: Quantitative Estimation of Sugars to Soft Drinks
Trials
Data Collection and Processing: Percentage Transmissions of Light through a Glucose Solution after a Benedicts Treatment

| Trial 1 | Trial 2 | Trial 3 | Trial 4 | Trial 5 | Trial 6 | Trial 7 | | Glucose Concentration | Transmission (%)± 0.1 | Transmission (%)± 0.1 | Transmission (%)±0.1 | Transmission (%)± 0.1 | Transmission(%)±0.1 | Transmission (%)±0.1 | Mean (anomalous data not included) ±0.1 | Standard Deviation | 0.00 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 0.0 | 0.25 | 83.8 | 99.9 | 85.9 | 80.9 | 5.8 | 90.9 | 88.3 | 7.5 | 0.50 | 84.8 | 73.0 | 75.5 | 52.4 | 5.2 | 83.3 | 73.8 | 13.0 | 0.75 | 85.1 | 90.6 | 67.3 | 71.5 | 5.9 |
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However there are many so

Inaccuracy | Effects | Improvement | Oxidation Reaction between glucose solution and benedicts solution | The reaction between the glucose and the benedict’s solution in the 70 degrees Celsius water-bath and this reaction could carry on after taking it out. | Cool it down in a beaker of ice water in order to slow down or stop the reaction from carrying on. | Precipitate | The reaction between glucose and Benedict’s solution produces a precipitate. This precipitate can be swirling around or sinking to the bottom possibly creating an unfair test between each concentration. | The simplest solution would just be to use a glass rod (after making sure it contains no other substances that may interfere with the solution) and stir the solution just before pouring it into the cuvette to make it a fair test for every glucose concentration. Filter paper could also be used. | Cuvette | Smudges or scratches on the cuvette could create serious inaccuracies in our results as this will interfere with the transmission of light. | If smudged, try and wipe off any marks from the cuvette. IF scratched, find another cuvette. | Water Bath Times | Due to the fact that occasionally we took the solutions out of the water bath too early or too late because of time restraints, this creates an unfair test as the time for the glucose concentrations to stay in the water bath should be exactly

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