Reaction of Catalase with Hydrogen Peroxide

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Reaction of catalase with hydrogen peroxide

AIM: I aim to find the rate of reaction between catalase and hydrogen peroxide.

Enzymes such as Catalase are protein molecules that are found in living cells. They are used to speed up specific reactions in the cells. Each enzyme just performs one particular reaction so they are all very specific. Catalase enzymes found in living cells e.g. in yeast, potato or liver, speed up (in our case) the breaking down of hydrogen peroxide.

The lock and key analogy…

The lock is the enzyme and its active sight is where you put the key in. The key is like the substrate that comes and bonds to the active site or the key that fits into the lock.

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The collision theory

The collision theory explains
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- Cardboard rectangle- to ensure no water escapes when tipping the 100ml measuring cylinder into the plastic tub.

METHOD

1. Collect all the equipment and set it up in a clear working space.
2. Fill the plastic tub about halfway to the top, with tap water.
3. Place the cardboard square on top of the 100ml measuring cylinder, making sure no water can get out; flip it over into the tub trying to loose as little water as possible. Your partner must keep the cylinder steady and vertical.
4. Using the 25ml measuring cylinder, measure 5cm³ of catalyse, and pour this into the conical flask. As we are using 5cm³ of catalase at the moment we do not need to dilute it, as the total solution is 5cm³. So seal your conical flask with a bung. (To make things easier you should collect the catalase and hydrogen peroxide in two beakers so that you find it easier to measure accurately from.)
5. Using your syringe you measure 5cm³ of hydrogen peroxide. Then place the syringe into the hole in the bung.
6. Now place the delivery tube that is attached to the bung underneath the 100ml measuring cylinder in the water.
7. Once this is done, you are ready to time your reaction. Push down on the syringe, adding the hydrogen peroxide to the catalase solution, and start your stopwatch. You should time how long it takes for the amount of water to reach 100ml to 0ml.

When you change the concentration for example to 3cm³ of
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