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Reactivity Of Hcl Acids With Cells Lab Report

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Reactivity of the HOCl acids with cells
The reactivity of HOCl has been comprehensively assessed with in a wide range of cellular systems, including yeast (Carmona-Gutierrez et al., 2013) and bacteria (Winterbourne and Kettle, 2013; Winterbourne et al., 2006; Chapman et al., 2002), together with many studies focused on specific mammalian cell types and tissues. It is clear from the numerous invitro studies that HOCl is highly reactive and destructive, inducing cell death via both necrotic and programmed apoptotic pathways, depending on the oxidant concentration and cell type under study.
HOCl-induced cell lysis
The a nucleate red blood cell (RBC) has been used extensively as a model system for investigating mechanisms of MPO-mediated cell …show more content…

Perhaps somewhat paradoxically, the continued exposure of macrophages to HOCl has been shown to exacerbate the inflammatory response in a number of disease settings, which is particularly relevant with in the progression of atherosclerosis (Daugherty et al., 1994). It has been shown that exposure of both murine macrophage cell lines and primary cultures of human monocyte-derived macrophages (HMDMs) to physiologically relevant concentrations of HOCl results in the loss of cell viability. In early studies employing the murine macrophage cell line P388D1 Schraufstätter and co-workers (Schraufstätter et al., 1990) demonstrated a dose-dependent increase in the extent of trypan blue uptake by the cells that was concurrent with the increased presence of lactate dehydrogenase (LDH) in cellular supernatants. Both of these methods are indicative of disruption to the cellular membrane and are used extensively as surrogate markers of cell death. In these studies, it was shown that exposure of cells to HOCl concentrations as low as 25 µM decreased cell viability by approximately 20% compared to controls, with 50 µM HOCl causing up to 50% cell death, and complete cell lysis observed after exposure to 75 µM HOCl. Although these data were

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