ction: What happens to red blood cells when treated with different sodium chloride (NaCl) solutions? For this experiment, sheep’s blood and three different NaCl solutions will be used. This is because the different solutions will give a clear range of different effects. Sheep’s blood is used because sheep are also mammals. The blood will be treated with the salt solutions and each slide will be viewed through a microscope. Diffusion is the movement of substances like oxygen from a higher concentration area (blood) to a lower concentration area (cell). Osmosis is the diffusion of solvents such as water through a cell’s membrane. For example, water is moved through channels created by aquaporins. These are found a lot in red blood cells. Active …show more content…
The same step was repeated for the other three slides. Slide 1 was moved to the side after a cover-slip was added to it. A drop of 0.9% NaCl was added to slide 2. A cover-slip was added after and filter paper was used to absorb the extra liquid on the slide. The blood contaminated items were placed on the mat throughout the experiment.
The microscope was set up accurately and slide 1 was placed onto the stage. The slide was observed under 40X, 100X and 400X (in order) for signs the cells had changed shape. The red blood cells observations were sketched and slide 2 was viewed under the microscope in the same format as slide 1. The observation on slide 2 was also sketched. Both slides 1 and 2 were placed in the beaker containing bleach.
A drop of 0% NaCl solution was then placed onto the third slide. A drop of 10% NaCl was added to slide 4 and cover-slips were placed over both slides. Slide 3 was observed under 40X, 100X and 400X (in order) for signs the cells had changed shape. The red blood cells observations were sketched and slide 4 was viewed under the microscope in the same format as slide 3. After slide 4 was also sketched, both slides were placed in the beaker containing bleach. The disposable items were then put in the solid biohazard waste container, including the gloves at the
To identify red blood cells, basophils, eosinophils, monocytes, lymphocytes, neutrophils, sickle cell anemia and leukemia.
Blood is a non-Newtonian fluid that contains many components. One such component is red blood cells. Due to the red blood cells having the tendency to clump together at low velocities, calcium chloride was added in order to cause a thrombus formation. The blood used in this experiment was sheep’s blood.
The guiding question relates to our background info because in order to know the effect the different solution had on the cell size we had to know our terminology, which was included in the background information. Section2: For this lab we had to follow certain procedures.
Explain what happened to the blood cells at the various levels of concentration. Be sure to refer to the solutions as being hypotonic, hypertonic and isotonic.
In this lab experiment, half our group observed and measured osmosis using dialysis tubes that were represented as the semipermeable membrane. It is permeable to water and other small molecules but is impermeable to larger molecules such as the sucrose solution used in each of the four beakers and tubing. The other half of our group observed the tonicity of sheep blood to determine whether the blood was isotonic, hypotonic, or hypertonic. The 85 g/dL of NaCl solution was the ideal isotonic number in relation to the sheep blood cells as well as a reference to the other observations of the solutions.
In this paper I will answer some questions about blood and related issues. Some of the questions I will answer are: what is the significance of a lower than normal haematocrit? what is erythropoiesis?why would the level of leukocytes be higher in an individual who has been infected with a parasitic disease. In regions where malaria is endemic, some people build up immune resistance to the malaria pathogen. Which WBCs are responsible for the immune response against pathogens? How do they function?
The goal of this experiment is to determine the blood types of the samples given and to learn what interactions occurred to each blood type. Determining an individual’s blood type and how it reacts with Anti A, Anti-B, and Anti Rh serums played a crucial part in this experiment. The researcher concluded that agglutination (clumping) occurred in some of the blood samples. For example, Mr. Smith’s blood reacted with Anti-A and Anti-Rh serums (antibodies) allowing the researcher to determine the blood type is A. Mr. Jones’s blood reacted with Anti-B serum but it did not react to Anti-A or Anti Rh allowing the researcher to believe that the blood type is B. Mr. Green’s blood reacted with all serums and caused a reaction to occur resulting the blood type to be AB positive. Mr. Green’s blood also had a positive marker for Rh factor. However, Ms. Brown’s blood had no reaction at all and the researcher determined if no reaction occurred then the sample had no antigens but proved to have some antibodies, resulting in blood type to be O. The purpose of this experiment is to determine whose blood has type A, B, AB, or O.
Cells and molecules in the environment are constantly moving and changing, for cells to function properly there is a need for equilibrium to be met. The size of the cell and the solution outside of the cell affects the rate of diffusion and osmosis in the cell. Cells are constantly trying to reach an equilibrium with the molecules and substances around it, which is why there are such terms as: hypertonic, hypotonic and isotonic. The procedures allowed testing of whether or not surface area or volume increased diffusion and how different substance control diffusion. Cells are constantly moving to reach equilibrium through diffusion and osmosis.
The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells, potato strips and three unknown solutions (A, B, C). First three slides were prepared containing RBC’s and unknown solutions A, B and C. A control slide was prepared only using RBC’s. After observing each slide under the microscope it was determined that unknown solution A was hypertonic because the RBC appeared to have shrunk. The RBC in unknown solution B appeared to be swollen, therefor, the tonicity of unknown solution B was hypotonic. Unknown solution C showed no change to the RBC shape, it was suggested that unknown solution C was isotonic. To confirm the tonicity
For this experiment student will identify the individual blood types through the antigen and antibody reaction by using the anti- A, Anti- B and Anti- Rh serums we will be able to identify the different types of blood. Red blood cells also known as erythrocyte are small cells shaped like an oval however they have a plasma membrane but no nucleus. Hemoglobin is the protein that allows the red blood cells to bind easily with oxygen. While hematopoiesis is known as blood cell formation. The independent variable would be the Red blood cells. While the dependent variable would be the Antibodies and the antigens
The first solution used is distilled water which is a hypotonic solution. In this situation, water will diffuse into the red blood cells causing them to expand and be round (as shown in the above results) and sometimes they may rapture as shown in the picture below:
A model cell was made of a visking tube filled with ‘cell contents’. The cell contents represent the cytoplasm of a cell structure membrane. The visking tube represents the plasma membrane of the cell. It acts as a semi-permeable barrier as
Descriptive elements such as this are used to portray the appearance of a positive hemochromatosis test so that the reader can visualize the view from a microscope. In all, extensive use of imagery strengthens this article by providing the reader with an accurate visualization
Analysis In the experiment, the scientist observed a series of cells, the first one being cheek cells. After swabbing the inside of their cheeks and preparing a slide, the scientists were able to see the cytoplasm, nucleus and cell membrane of this undyed cell. While observing these cells under 400X, they noticed that the cheek cells varied in shape, some being almost perfectly spherical, while others resembled an oval figure. Additionally, these cells appeared to be grouped up and messily stacked on top of one another.