Replication Of Hev Is Still Largely Unknown Because Effective Cell Culture System For Hev
1457 WordsDec 4, 20166 Pages
Replication of HEV
Replication pattern of HEV is still largely unknown because effective cell-culture system for HEV has been developed recently (Tanaka et al., 2007). It is believed that HEV enters the cell by binding to cellular receptor, heparin sulfate proteoglycans (HSPGs), with its capsid protein and viral entry is carried out by the binding of C-terminal region of ORF2 to the cell surface protein, heat shock cognate protein 70 (HSC70) (Zhou and Emerson, 2006; Kalia et al., 2009). Also, p239, a truncated partial homodimer of the HEV capsid protein (HEV239) potentially binds to the HEV receptor(s) on cells (He et al., 2008) and a cellular chaperone, glucose regulated protein (Grp78) (Yu et al., 2011). However, more studies should be conducted to confirm the cellular receptors for HEV. The mechanism of uncoating of the HEV genome after its entry into the cells is also unknown. One of the study has suggested that heat shock protein (HSP90) might be playing role in intracellular transport of HEV since the inhibition of HSP90 blocked the intracellular transport of the HEV239 capsid protein but did not affect the binding and entry of the truncated capsid protein (Zheng et al., 2010).
After uncoating and entry of viral genome, the 5′ 7-methylguanosine cap in NCR of the positive